Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP Fast Multiplex Assays for Detection of Respiratory Viruses

Popowitch, Elena B.; O’Neill, Stacey S.; Miller, Melissa B.

doi:10.1128/jcm.03368-12

Cited by 223 publications

(197 citation statements)

References 23 publications

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“…These findings suggest that the discordant results were frequently reported in samples containing low viral copy. Other studies also indicated that discrepant results between RV assay was quite associated with sample's viral load 7, 8, 19, 20, 21. One samples by AP and three samples by AD identified as INF A positive by additional testing.…”

Section: Discussionmentioning

confidence: 97%

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Yun

Kim

Choi

et al. 2017

Clinical Laboratory Analysis

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BackgroundDue to its great sensitivity, the nucleic acid amplification test (NAAT) is widely used for detection of respiratory viruses (RV). However, few reports have described a direct comparison between multiplex RT‐PCR assays for RV. The objective of this study was to perform a direct comparison of three multiplex RT‐PCR assays for the detection of respiratory viruses.MethodsA total of 201 respiratory samples (161 nasopharyngeal swab samples and 40 sputum samples) were tested with three commercial RV assays: Seegene Anyplex II RV16 (AP), LG AdvanSure RV (AD), and Biosewoom Real‐Q RV (RQ). The additional tests for the discrepant results were conducted by repeat RV assay or monoplex PCR coupled direct sequencing. Data analysis using percent agreement, kappa, and prevalence‐adjusted and bias‐adjusted kappa (PABAK) values was performed for comparisons among the three RV assays.ResultsOf the 201 samples, AP, AD, and RQ detected 105 (52.2%), 99 (49.3%), and 95 (47.3%) positive cases respectively. The overall agreement, kappa, and PABAK values for the three assays ranged between 97%‐98%, 0.76‐0.86, and 0.93‐0.96 respectively. The performance of the three assays was very similar, with 94%‐100% agreement for all comparisons, each virus types. The additional testing of samples showed discrepant results demonstrating that AD assay had the highest rate of concordance with original results.ConclusionsWe suggest that all multiplex assay would be suitable for the detection of for respiratory viruses in clinical setting.

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Section: Discussionmentioning

confidence: 97%

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Yun

Kim

Choi

et al. 2017

Clinical Laboratory Analysis

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“…Most of the indicated kits cover multiple viruses and are only approved for qualitative analysis of respiratory specimens. Studies comparing the performances of such kits indicated a particularly high variability for HAdV detection, with inadequate identification of certain HAdV types by some of the tests (196)(197)(198). The monitoring of patients in the immunocompromised setting requires tests permitting reliable detection of all potentially relevant HAdV types in different clinical specimens, as well as accurate quantitative assessment of viral loads.…”

Section: Diagnostic Screeningmentioning

confidence: 99%

Adenovirus Infections in Immunocompetent and Immunocompromised Patients

2014

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SUMMARY Human adenoviruses (HAdVs) are an important cause of infections in both immunocompetent and immunocompromised individuals, and they continue to provide clinical challenges pertaining to diagnostics and treatment. The growing number of HAdV types identified by genomic analysis, as well as the improved understanding of the sites of viral persistence and reactivation, requires continuous adaptions of diagnostic approaches to facilitate timely detection and monitoring of HAdV infections. In view of the clinical relevance of life-threatening HAdV diseases in the immunocompromised setting, there is an urgent need for highly effective treatment modalities lacking major side effects. The present review summarizes the recent progress in the understanding and management of HAdV infections.

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“…Although sensitivity and specificities vary between different RVPs such as the BioFire Film Array, GenMark eSensor, Luminex xTAG, and the new NxTAG, a significant advantage is the detection of multiple respiratory pathogens (Table 1). 14 …”

Section: Discussionmentioning

confidence: 99%

The assessment of data sources for influenza virologic surveillance in New York State

Escuyer

Waters

Gowie

et al. 2016

Influenza Resp Viruses

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BackgroundFollowing the 2013 USA release of the Influenza Virologic Surveillance Right Size Roadmap, the New York State Department of Health (NYSDOH) embarked on an evaluation of data sources for influenza virologic surveillance.ObjectiveTo assess NYS data sources, additional to data generated by the state public health laboratory (PHL), which could enhance influenza surveillance at the state and national level.MethodsPotential sources of laboratory test data for influenza were analyzed for quantity and quality. Computer models, designed to assess sample sizes and the confidence of data for statistical representation of influenza activity, were used to compare PHL test data to results from clinical and commercial laboratories, reported between June 8, 2013 and May 31, 2014.ResultsSample sizes tested for influenza at the state PHL were sufficient for situational awareness surveillance with optimal confidence levels, only during peak weeks of the influenza season. Influenza data pooled from NYS PHLs and clinical laboratories generated optimal confidence levels for situational awareness throughout the influenza season. For novel influenza virus detection in NYS, combined real‐time (rt) RT‐PCR data from state and regional PHLs achieved ≥85% confidence during peak influenza activity, and ≥95% confidence for most of low season and all of off‐season.ConclusionsIn NYS, combined data from clinical, commercial, and public health laboratories generated optimal influenza surveillance for situational awareness throughout the season. Statistical confidence for novel virus detection, which is reliant on only PHL data, was achieved for most of the year.

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Comparison of the Biofire FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVPv1, and Luminex xTAG RVP Fast Multiplex Assays for Detection of Respiratory Viruses

Cited by 223 publications

References 23 publications

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Adenovirus Infections in Immunocompetent and Immunocompromised Patients

The assessment of data sources for influenza virologic surveillance in New York State

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