Comparison of three multiplex <scp>PCR</scp> assays for detection of respiratory viruses: Anyplex <scp>II RV</scp>16, AdvanSure <scp>RV</scp>, and Real‐Q <scp>RV</scp>

Yun, Seung Gyu; Kim, Min Young; Choi, Jong Moon; Lee, Chang Kyu; Lim, Chae Seung; Cho, Yunjung; Suh, In Bum

doi:10.1002/jcla.22230

Cited by 17 publications

(15 citation statements)

References 23 publications

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“…After obtaining the ethical approval, we retrospectively reviewed charts of 387 patients with cystic fibrosis of all age groups who were screened for detection of viruses during respiratory exacerbation from January 1, 1984 to June 1, 2016. Polymerase chain reaction (PCR) of respiratory viruses were extracted and analyzed according to the standard method [10,11].…”

Section: Methodsmentioning

confidence: 99%

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The prevalence of viral infections in children with cystic fibrosis in a tertiary care center in Saudi Arabia

Banjar

Chaballout

Karkour

et al. 2020

International Journal of Pediatrics and Adolescent Medicine

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Introduction: Studies have shown that pulmonary exacerbations in cystic fibrosis (CF) patients are associated with respiratory viruses. The most common agent causing viral infections in patients with CF before the age of 3 years is respiratory syncytial virus. Objectives: To obtain the prevalence of the different types of viral infection in CF patients and to identify its relation with the type of bacterial infection, (CFTR) mutations and pulmonary function test (PFT). Methodology: A retrospective charts review of 387 patients with CF of all age groups who were screened for the detection of viruses during respiratory exacerbation from the period of January 1,1984 to June 1, 2016. Results: A total of 159 CF patients had pulmonary exacerbation and had viral PCR obtained. Fifty-eight patients (36%) had positive viral PCR. Males were more commonly infected in 30/58 patients (52%) compared to females in 28 patients (48%). Forty-five of 58 patients (78%) were alive and 13 patients (22%) died. Rhinovirus was the most frequently reported viral PCR in 33/74 sample (45%). Out of 74 viral PCR, 41 (55.4%) were during the colder seasons (October–February) and 33 (44.5%) during the warmer seasons (March–September). During viral infection and viral recurrence, there was an increase in bacterial colonization specifically of H. influenza and staphylococcus aureus. The most common CFTR mutation for the CF viral infection is: 3120+1G>A in Intron 16 in 11/57 patients (19%). The Eastern Province had the highest viral infection of 24 out of 57 patients (42%). Follow-up PFT post viral infection showed no significant difference in the type and the severity of PFT compared to the initial PFT during the viral illness. Conclusion: Viral infections contributed to the increase in morbidity and mortality of CF patients in our population, and rhinovirus was the most common causative agent. Viral infections and viral recurrence increased the prevalence of bacterial infection of specific pathogens such as H. in fluenza and S. aureus. Physicians should be aware to prevent progressive lung damage in CF patients by treating the concomitant viral and bacterial infections. Viral infection may be associated with some common CFTR mutations.

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Section: Methodsmentioning

confidence: 99%

“…All CF patients of all ages who underwent PCR multiplex for respiratory viruses during their follow up from the period January 1, 1984 to June 1, 2016 were included [10,11].…”

Section: Inclusion Criteriamentioning

confidence: 99%

The prevalence of viral infections in children with cystic fibrosis in a tertiary care center in Saudi Arabia

Banjar

Chaballout

Karkour

et al. 2020

International Journal of Pediatrics and Adolescent Medicine

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“…The overall PDR was 26.8%, with influenza and RSV accounting for most test-positive specimens. Comparing the overall and virus-specific PDR between studies is made problematic by the variability of factors like the demographic structure of the country, the surveillance scheme and the clinical features of patients being sampled, the sampling techniques being used [15], and the laboratory procedures and assays (which may vary in terms of number of respiratory pathogens that can be detected) [16]. By and large, a viral aetiology could be established in a smaller number of patients compared to previous studies [5,[17][18][19][20], which might be explained by the failure to detect common causes of RVIs like rhinovirus, coronavirus, and others [21].…”

Section: Discussionmentioning

confidence: 99%

The epidemiology and severity of respiratory viral infections in a tropical country: Ecuador, 2009–2016

Caini¹,

Mora²,

Olmedo³

et al. 2019

Journal of Infection and Public Health

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a b s t r a c tBackground: Respiratory viral infections (RVI) are a leading cause of mortality worldwide. We compared the epidemiology and severity of RVI in Ecuador during 2009-2016. Methods: Respiratory specimens collected within the national surveillance system were tested for influenza viruses, respiratory syncytial virus (RSV), adenovirus, parainfluenza virus, and human metapneumovirus. Overall and virus-specific positive detection rate (PDR) were calculated and compared the timing of epidemics caused by the different viruses. Logistic regression models were used to compare the age distribution and risk of death across respiratory viruses. Results: A total of 41,172 specimens were analyzed: influenza (PDR = 14.3%) and respiratory syncytial virus (RSV) (PDR = 9.5%) were the most frequently detected viruses. Influenza epidemics typically peaked in December-January and RSV epidemics in March; seasonality was less evident for the other viruses. Compared to adults, children were more frequently infected with RSV, adenovirus, parainfluenza, and influenza B, while the elderly were less frequently infected with influenza A(H1N1)p. The age-adjusted risk of death was highest for A(H1N1)p (odds ratio [OR] 1.73, 95% confidence intervals [CI] 1.38-2.17), and lowest for RSV (OR 0.75, 95%CI 0.57-0.98). Conclusions: Whilst influenza and RSV were the most frequently detected pathogens, the risk of death differed by RVI, being highest for pandemic influenza and lowest for RSV.

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“…Nucleic acid—based methods are often preferred for the diagnosis of viral respiratory infections, especially for hospitalized patients. These tests quickly identify the agent with high sensitivity and help to facilitate the appropriate management for both the patient and the infection control measures . Specific polymerase chain reactions (PCRs; monoplex) can be used to identify single viral agents such as influenza A/B and RSV, however, their role is limited.…”

Section: Introductionmentioning

confidence: 99%

Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients

Appak

Duman

Belet

et al. 2019

Journal of Medical Virology

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Syndromic diagnosis by multiplex nucleic acid amplification tests is the most practical approach to respiratory tract infections since the symptoms are rarely agent‐specific. The aim of this study was to investigate the respiratory viruses in children admitted to a university hospital with acute respiratory tract infection during the last 8 years by a multiplex polymerase chain reaction (PCR) assay. A total of 3162 respiratory samples collected from children between April 2011 and April 2018 tested by a multiplex real‐time PCR assay. Two different commercial assays were used during the study period, "AusDiagnostics/Respiratory Pathogens 12 (AusDiagnostics)" used between April 2011 and December 2015, which changed to "Fast Track Diagnostics/Respiratory Pathogens 21 (Fast Track Diagnostics)" after January 2016 to cover more viruses. Nucleic acid extraction was done by EZ1 Advanced XL platform (QIAGEN). Respiratory pathogens detected in 1857 of the 3162 (58.7%) samples. The most prevalent viruses during the 8‐year period were rhinovirus/enterovirus (RV/EV; 36.2%), respiratory syncytial virus (RSV; 19%), and influenza virus A/B (14.7%). Rhinovirus was the main contributor to the RV/EV group as shown by the assay used during the 2016‐2018 period. RV/EV and adenoviruses detected throughout the year. Influenza virus was most frequently detected during January to March when both RSV and metapneumovirus were also in circulation. The coinfection percentage was 10.2%. Rhinovirus was the most common virus in coinfections while RSV plus rhinovirus/enterovirus were the most frequent combination. RSV and metapneumovirus showed a similar seasonal distribution to the influenza virus, which made it necessary to use a virological diagnostic assay during the influenza season.

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Comparison of three multiplex PCR assays for detection of respiratory viruses: Anyplex II RV16, AdvanSure RV, and Real‐Q RV

Cited by 17 publications

References 23 publications

The prevalence of viral infections in children with cystic fibrosis in a tertiary care center in Saudi Arabia

The prevalence of viral infections in children with cystic fibrosis in a tertiary care center in Saudi Arabia

The epidemiology and severity of respiratory viral infections in a tropical country: Ecuador, 2009–2016

Viral respiratory infections diagnosed by multiplex polymerase chain reaction in pediatric patients

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