Comparison of the Automated Random Access Immunoassay Analysers, ACS-180 (Ciba Corning) and AIA-1200 (Tosoh)

Costongs, G. M. P. J.; Janson, Paul

doi:10.1515/cclm.1993.31.10.701

Cited by 3 publications

(3 citation statements)

References 5 publications

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“…Results were evaluated by the non-parametric regression procedure of Passing & Bablok (8). Tlie concentrations were distributed within the expected clinical thyrotropin range, and they included low and high values.…”

Section: Limit Of Detectionmentioning

confidence: 99%

Technical Reports

1995

CCLM

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We evaluated the technical performance of two new commercial automated immunoassay systems of third-generation assays for thyrotropin. The interassay CV was 2.8% for AutoDELFIA and 3.25% for Access at thyrotropin concentrations of approximately 1.3 mIU/1. The lower detection limits of the assays were 0.023 mIU/1 for AutoDELFIA and 0.0096 mIU/1 for Access, and the functional sensitivity for a CV of 20% was 0.027 mIU/1 and 0.028 mIU/1, respectively. Sample to sample carry over was negligible (0.0016% for AutoDELFIA and 0.005% for Access). The range of linearity was acceptable for Access (102-115%) but not for low thyrotropin concentrations in AutoDELFIA (143% for a thyrotropin value of 0.48 mIU/1). Correlation between AutoDELFIA and Access was adequate (r = 0.999). We conclude that both automated immunoassays offer good reliability, practicability and performance characteristics.The technology used is an automated adaptation of the DELFIA manual equipment. The improved lower detection limit obtained with the thyrotropin test is due to the use of three monoclonal antibodies directed against three separate antigenic determinants of the thyrotropin molecule.Summary: The automated immunoassay analyser, IMMULITE, developed by DPC, was evaluated. IMMULITE is an automated system that allows random access in combination with immediate and continuous access. In this study we evaluated the IMMULITE on four panels of analytes: thyroid, fertility, tumour and "non-routine" markers.We observed good within-run reproducibility (ranging from 2.3-15.9%CV, for low controls, to 2.7-8.7%CV for high controls) as well as between-day imprecision (ranging from 3.7-24.6%CV for low controls, to 2.5-11.8%CV for high controls).The analytical sensitivity of the assays ranged generally from very sensitive to acceptable.The dilution curves for all assays were nearly linear i. e. the maximum deviation of the observed from the expected 1 recovery was 8%. . I \Correlation between IMMULITE and other assays (AxSYM, IMx, TDx, AIA-1200, DPC-c. a. c., Medgenix) varied i, from r = 0.931 to r = 0.994, except for lutropin and parathyrin with coefficients of correlation of r = 0.594 and r = 0.591. The slopes of the regression lines ranged from 0.745 to 1.327, except for parathyrin where a slope of 2.389 was found. Inter-laboratory correlation was very good between the two locations (Sittard and Apeldoorn) and varied from r = 0.984 to r = 0.999; slopes of the regression lines varied from 0.924 to 1.086. We conclude that the DPC JMMULITE system is suitable for testing routine analytes in random access mode as well as for testing "non-routine" analytes on an automated immunoassay analyser.

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Section: Limit Of Detectionmentioning

confidence: 99%

Technical Reports

1995

CCLM

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“…To avoid tedious chromatographic separations, a rapid and precise immunoassay was developed based on enzymatic conversion of Hcy to S-adenosyl-L-homocysteine (SAH) by the action of SAH hydrolase, followed by quantification of SAH in a competitive immunoassay with use of an anti-SAH antibody [15]. Moreover, this principle has been used in an automated tHcy assay on the Abbott IMx intstrument and AxSYM analyzer [16][17][18]. These completely automated methods use expensive equipments while those need less time and have good accuracy and precision.…”

Section: Introductionmentioning

confidence: 99%

Reagent-loaded plastic microfluidic chips for detecting homocysteine

Suk

Jang²,

Cho³

2008

J. Micromech. Microeng.

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This report describes the preliminary study on plastic microfluidic chips with pre-loaded reagents for detecting homocysteine (Hcy). All reagents needed in an Hcy immunoassay were included in a microfluidic chip to remove tedious assay steps. A simple and cost-effective bonding method was developed to realize reagent-loaded microfluidic chips. This technique uses an intermediate layer between two plastic substrates by selectively patterning polydimethylsiloxane (PDMS) on the embossed surface of microchannels and fixing the substrates under pressure. Using this bonding method, the competitive immunoassay for SAH, a converted form of Hcy, was performed without any damage to reagents in chips, and the results showed that the fluorescent signal from antibody antigen binding decreased as the SAH concentration increased. Based on the SAH immunoassay, whole immunoassay steps for Hcy detection were carried out in plastic microfluidic chips with all necessary reagents. These experiments demonstrated the feasibility of the Hcy immunoassay in microfluidic devices.

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“…Sample carryover is a potential hazard for laboratory parameters sharing a large dynamic range. Published reports in regard to the determination of TSH describe no significant carryover (Costongs and Janson, 1993, Khalil et al, 1991, Hebles-Duvison et al, 1995, Stockmann et al, 1998, Letellier et al, 1996, Forrest et al, 1998, Knedel et al, 1988, DCosta et al, 1993. An estimated specimen carryover of 1:1250 to 1:1160 has been reported for pipetting stations using fixed pipettes (Sadler et al, 1996, European Committee for Clinical Laboratory Standards.…”

Section: Introductionmentioning

confidence: 99%

Spuriously high serum concentrations of TSH due to the use of an automatic pipetting device

Gessi¹,

Raber²,

Nowotny³

et al. 2000

Exp Clin Endocrinol Diabetes

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In a clinically hyperthyroid patient a TSH-value of 0.75 mU/L was measured. Upon retesting TSH was <0.1 mU/L. The patient's sample initially had been preceded, in the pipetting device, by a grossly hypothyroid patient's sample. Analogous problems were subsequently documented in 10 cases when samples of other hyperthyroid patients were preceded by samples with modestly to markedly elevated TSH. The spurious elevation of TSH (0.12 to 1.18 vs. <0.1 mU/L) appears to be due to a carryover effect from the respective previous sample within the used automatic pipetting system. This carryover was much larger than previously suggested, and occurred unpredictably.

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Comparison of the Automated Random Access Immunoassay Analysers, ACS-180 (Ciba Corning) and AIA-1200 (Tosoh)

Cited by 3 publications

References 5 publications

Technical Reports

Technical Reports

Reagent-loaded plastic microfluidic chips for detecting homocysteine

Spuriously high serum concentrations of TSH due to the use of an automatic pipetting device

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