Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents

Jia, Na; Li, Yuwen; Wu, Yin; Xi, Miaomiao; Hur, Gang Min; Zhang, Xinxin; Cui, Jia; Sun, Wen-Ji; Wen, Aidong

doi:10.1016/j.jep.2012.10.004

Cited by 61 publications

(52 citation statements)

References 28 publications

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“…It is generally accepted that acetic acid acts by increasing endogenous mediators, such as prostaglandin and histamine, in peritoneal fluids, leading to stimulation of nociceptive neurons. 17) The abdominal constriction response is postulated to be induced by the activation of local peritoneal receptors. 23) Administration of EMP reduced both the latency time and the number of acetic acid-induced abdominal constrictions, indicating analgesic activity.…”

Section: Discussionmentioning

confidence: 99%

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Antiinflammatory and Analgesic Activities of Ethanol Extract and Isolated Compounds from Millettia pulchra

Huo

Zhang

Gao

et al. 2015

Biological & Pharmaceutical Bulletin

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The plant Millettia pulchra was commonly used in folk medicine for the management of inflammation. However, there was no scientific rationale for these effects and the mechanism of action remained incompletely understood. The present study was designed to investigate the antiinflammatory and analgesic activities of an ethanol extract of the stem of M. pulchra (EMP) in vivo, and to explore the antiinflammatory activity of compounds isolated from EMP in vitro. We found that EMP reduced xylene-induced ear edema and relieved both acetic acid-induced pain and pain in the hot plate test. Additionally, a significant decrease in nitric oxide (NO) production was observed in cells treated with the isolated compounds. Lanceolatin B, which showed the greatest inhibition of NO synthesis among the compounds tested, also reduced levels of interleukin-1 beta (IL-1β), IL-6, tumor necrosis factor-alpha (TNF-α), cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), nuclear factor-kappa B (NF-κB), and phosphorylation inhibitory kappa B alpha (p-IκBα) in a dose-dependent manner. These findings provide convincing evidence that EMP and the individual isolated compounds possess significant antiinflammatory and analgesic activities.

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Section: Discussionmentioning

confidence: 99%

“…16,17) In this test, mice were placed on the hot-plate maintained at 55±0.5°C. The time that elapsed until the occurrence of either a hind paw licking or a jump off the surface was recorded as the latency of pain response.…”

Section: Antiinflammatory and Analgesic Activities In Vivomentioning

confidence: 99%

Antiinflammatory and Analgesic Activities of Ethanol Extract and Isolated Compounds from Millettia pulchra

Huo

Zhang

Gao

et al. 2015

Biological & Pharmaceutical Bulletin

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“…The roots of G. macrophylla taste bitter due to the presence of some phyto-constituents. Studies have shown that G. macrophylla extracts possess laxative properties, and its ethanol extract exhibits broad range antimicrobial activity [6]. Often, G. macrophylla, is used in combination with other Gentiana species for maintenance of healthy sinus, and for treating rheumatoid arthritis and osteoporosis [7].…”

Section: Gentiana Macrophylla Belongs To Familymentioning

confidence: 99%

Gentiana macrophylla Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

Gong¹,

Su²,

Xiang³

et al. 2018

Trop. J. Pharm Res

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Purpose: To evaluate the anti-osteoporotic effect of G. macrophylla extract in a mice model. Methods: Antioxidant activity was determined by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) for radical scavenging, nitro-blue tetrazolium (NBT) reducing, and ferric reducing antioxidant power (FRAP) assays using standard biochemical procedures. The expression of some bone-related genes was measured by quantitative real-time polymerase chain reaction (qTR-PCR). Phytochemical analysis was carried out by liquid chromatography-mass spectrometry (LC/MS

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“…Acetic acid stimulates pain neurons by increasing endogenous mediators such as PG, leukotriene (LT), and histamine in peritoneal fluids (Jia et al, 2012). Mitigation of the acetic-acid induced writhing response has been associated with decreased synthesis of inflammatory factors via the inhibition of COX or lipoxygenase (Franzotti et al, 2000;Ojewole, 2006).…”

Section: Introductionmentioning

confidence: 99%

Maydis Stigma Elicits Analgesia and Blocks Edema in Mice and Inhibits Inflammation in Macrophages

Jeong

Cho

et al. 2017

Am. J. Chin. Med.

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Maydis Stigma (MS) is an herb traditionally used in many parts of the world. Previous studies have reported that MS plays a role in several biological activities, including antidiabetic and anticancer activities. However, the effects of a MS ethanolic extract (MSE) on the anti-inflammatory cellular mechanism remain unclear. Here, we investigated the antiinflammatory properties of MSE and its molecular mechanism both in vitro and in vivo. The effects of MSE on the production of inflammatory mediators, cytokines, and related proteins and the identification of target genes were determined using LPS-stimulated macrophages. We also determined the analgesic and anti-inflammatory effects of MSE by examining acetic acid-induced writhing responses and xylene-induced ear edema in mice. Our results indicated that MSE markedly decreased iNOS and COX-2 levels without causing cytotoxicity and suppressed the secretion of NO in LPS-stimulated macrophages. MSE also inhibited the production of proinflammatory cytokines, such as TNF-α, IL-6, and IL-1β, and induced the expression of HO-1. Moreover, MSE treatment significantly reduced the LPS-stimulated activation of MAPK, NF-κB, and AP-1. Furthermore, MSE exerted an analgesic effect on the acetic acid-induced abdominal writhing response test and an anti-inflammatory effect on xylene-induced ear edema in ICR mice. Finally, we investigated the components of MSE using UPLC-ESI-MS and found that it contains the maysin as a marker component. Overall, these observations demonstrate that MSE has anti-inflammatory and antinociceptive effects both in vitro and in vivo, which may provide new scientific evidence for its use as a potential therapeutic agent for the treatment of inflammation.

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Comparison of the anti-inflammatory and analgesic effects of Gentiana macrophylla Pall. and Gentiana straminea Maxim., and identification of their active constituents

Cited by 61 publications

References 28 publications

Antiinflammatory and Analgesic Activities of Ethanol Extract and Isolated Compounds from <i>Millettia pulchra</i>

Antiinflammatory and Analgesic Activities of Ethanol Extract and Isolated Compounds from <i>Millettia pulchra</i>

<i>Gentiana macrophylla</i> Pall (Gentianaceae) extract exerts protective effects against osteoporosis in mice

Maydis Stigma Elicits Analgesia and Blocks Edema in Mice and Inhibits Inflammation in Macrophages

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