Comparison of the Agronomic, Cytological, Grain Protein Characteristics, as Well as Transcriptomic Profile of Two Wheat Lines Derived From Wild Emmer

Gong, Fangyi; Qi, Tiangang; Zhang, Tian; Lu, Yusen; Liu, Jia; Zhong, Xu; He, Jingshu; Li, Yunfang; Zheng, Youliang; Liu, Dengcai; Huang, Lin; Wu, Bihua

doi:10.3389/fgene.2021.804481

Cited by 4 publications

(5 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on the RNA-seq database [ 32 ] owned by our laboratory, the expression patterns of TdNAC genes in developing grains were further analyzed. One hundred and three of 249 TdNAC genes were expressed (FPKM ≥ 1), and 50 TdNAC genes were highly expressed (FPKM ≥ 5) in either grains of wild emmer D97 (high grain protein content) or common wheat CN16 (low grain protein content) ( Figure S6, Table S3 ).…”

Section: Resultsmentioning

confidence: 99%

“…Wild emmer wheat ( T. turgidum ssp. dicoccoides , 2n = 4x = 28, AABB) is the tetraploid ancestor of common wheat and provides a valuable reservoir of genetic variation for grain protein content [ 16 ], disease resistance [ 30 ] and grain micronutrient content [ 31 , 32 ]. Although the NAC TF family has been widely studied in cereal crops, the significance of the NAC TF family in wild emmer is not yet well understood.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat

Gong

Zhang

Wang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The NAC transcription factor (TF) family is one of the largest TF families in plants, which has been widely reported in rice, maize and common wheat. However, the significance of the NAC TF family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well understood. In this study, a genome-wide investigation of NAC genes was conducted in the wild emmer genome and 249 NAC family members (TdNACs) were identified. The results showed that all of these genes contained NAM/NAC-conserved domains and most of them were predicted to be located on the nucleus. Phylogenetic analysis showed that these 249 TdNACs can be classified into seven clades, which are likely to be involved in the regulation of grain protein content, starch synthesis and response to biotic and abiotic stresses. Expression pattern analysis revealed that TdNACs were highly expressed in different wheat tissues such as grain, root, leaves and shoots. We found that TdNAC8470 was phylogenetically close to NAC genes that regulate either grain protein or starch accumulation. Overexpression of TdNAC8470 in rice showed increased grain starch concentration but decreased grain Fe, Zn and Mn contents compared with wild-type plants. Protein interaction analysis indicated that TdNAC8470 might interact with granule-bound starch synthase 1 (TdGBSS1) to regulate grain starch accumulation. Our work provides a comprehensive understanding of the NAC TFs family in wild emmer wheat and establishes the way for future functional analysis and genetic improvement of increasing grain starch content in wheat.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat

Gong

Zhang

Wang

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The advanced wheat line BAd7-209 ( T. aestivum CN16/ T. dicoccoides D97 F 12 ) [ 61 ] and transgenic rice ( Oryza. Sativa L. spp.…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat

Gong

Qi²,

Hu³

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

The zinc/iron-regulated transporter-like protein (ZIP) family has a crucial role in Zn homeostasis of plants. Although the ZIP genes have been systematically studied in many plant species, the significance of this family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well understood. In this study, a genome-wide investigation of ZIPs genes based on the wild emmer reference genome was conducted, and 33 TdZIP genes were identified. Protein structure analysis revealed that TdZIP proteins had 1 to 13 transmembrane (TM) domains and most of them were predicted to be located on the plasma membrane. These TdZIPs can be classified into three clades in a phylogenetic tree. They were annotated as being involved in inorganic ion transport and metabolism. Cis-acting analysis showed that several elements were involved in hormone, stresses, grain-filling, and plant development. Expression pattern analysis indicated that TdZIP genes were highly expressed in different tissues. TdZIP genes showed different expression patterns in response to Zn deficiency and that 11 genes were significantly induced in either roots or both roots and shoots of Zn-deficient plants. Yeast complementation analysis showed that TdZIP1A-3, TdZIP6B-1, TdZIP6B-2, TdZIP7A-3, and TdZIP7B-2 have the capacity to transport Zn. Overexpression of TdZIP6B-1 in rice showed increased Zn concentration in roots compared with wild-type plants. The expression levels of TdZIP6B-1 in transgenic rice were upregulated in normal Zn concentration compared to that of no Zn. This work provides a comprehensive understanding of the ZIP gene family in wild emmer wheat and paves the way for future functional analysis and genetic improvement of Zn deficiency tolerance in wheat.

show abstract

“…New subunits (1Ax2.2 and 1Ay) were recorded in the wild emmer accession D97. The 1Ax1.2 protein fraction was introduced into one of two newly obtained lines from the cross of the common wheat variety Chuannong 16 with the wild strain emmer [61]. Adding to the list of new genes is Glu-A1x, found in native wheat cultivars, encoding a subunit called 1‴.…”

Section: Tetraploid and Hexaploid Triticum Speciesmentioning

confidence: 99%

Allelic Variation of High-Molecular-Weight Glutenin Genes in Triticum Species and Triticale (× Triticosecale Wittmack)

Spetsov,

Daskalova

2024

OBM Genet

View full text Add to dashboard Cite

High-molecular-weight glutenin subunits (HMW-GS) encoded by alleles at the Glu-A1, Glu-B1, and Glu-D1 loci confer unique bread-making properties of common wheat (Triticum aestivum L.). The identification of HMW-GS is the prerequisite for pyramiding high-quality glutenin genes. The present review is designed to list all published HMW-GS alleles in Triticum species and triticale (A- and B genomes), focusing on methods for their identification. T. monococcum is characterized by 37 alleles at the Glu-1 locus versus four alleles in T. thaoudar and 39 in T. urartu. In total, 80 alleles at Glu-A1 of diploid Triticum species and about 42 alleles found in polyploid wheat landraces and varieties (4x and 6x), including triticale, were listed. Allelic variation at the Glu-B1 locus is divided into 3 groups: a – z, aa – az, and ba – ct, comprising 121 alleles, of which 26 subunits have unspecified alleles. At least 51 allelic variants at locus Glu-D1 of Triticum species were indicated, along with carriers of the species level. In addition, subunit-specific genetic loci have been tagged, facilitating molecular marker development of high-gluten wheat cultivars through marker-assisted breeding.

show abstract

Comparison of the Agronomic, Cytological, Grain Protein Characteristics, as Well as Transcriptomic Profile of Two Wheat Lines Derived From Wild Emmer

Cited by 4 publications

References 74 publications

Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat

Genome-Wide Survey and Functional Verification of the NAC Transcription Factor Family in Wild Emmer Wheat

Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat

Allelic Variation of High-Molecular-Weight Glutenin Genes in <i>Triticum</i> Species and Triticale (× <i>Triticosecale</i> Wittmack)

Contact Info

Product

Resources

About