Comparison of the 2000 and 2005 spatial distributions of heavy metals in wild mussels from the North-Atlantic Spanish coast

Proteomic profiling of gill GSTs in Mytilus galloprovincialis from the North of Portugal and Galicia evidences variations at protein isoform level with a possible relation with water quality

“…In each region, one site affected by intense anthropogenic activity (polluted) was chosen, as well as one secluded (clean) site. The three referenced contaminated sampling sites, port of Vigo, 42.228753N, 8.761500W (polluted 1, P1), port of Viana do Castelo, 41.679828N, 8.836414W (polluted 2, P2) and port of Leixões, in Matosinhos, 41.183427N, 8.698586W (polluted 3, P3), are all located in urban centres, very close to intense port activity, being thus affected by different sources of contamination from fuels, oil spills, vessels' coating materials, etc (Bellas et al, 2011;Besada et al, 2011aBesada et al, , 2011bLima et al, 2007;Cairrão et al, 2004;Cunha et al, 2005) (Fig. 1).…”

Influence of mussel biological variability on pollution biomarkers

“…Samples for metals analyzes were digested with nitric acid (Suprapur, Merck) in a microwave oven (Besada et al, 2011). Briefly, freeze-dried mussel samples were placed in a high pressure Teflon reactor, and after the addition of the nitric acid, digested in a microwave oven at 90°C for 10 min and then at 180°C for 60 min.…”

Relationships between polychlorinated biphenyls in molluscs, hydrological characteristics and human pressures, within Basque estuaries (northern Spain)

“…developed world-wide (e.g., Monirith et al, 2003;Kimbrough et al, 2008;Besada et al, 2011;IFREMER, 2011;OSPAR, 2012).…”