Comparison of Tetraacetylethylendiamine + Sodium Perborate and Sodium Hypochlorite Cytotoxicity on L929 Fibroblasts

Simbula, Gabriella; Dettori, Claudia; Camboni, Tania; Cotti, Elisabetta

doi:10.1016/j.joen.2010.05.010

Cited by 23 publications

(22 citation statements)

References 17 publications

(22 reference statements)

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“…This is consistent with the results of previous studies that reported on NaOCl toxicity [23,32,33]. NaOCl toxicity can be attributed to its high pH (hydroxyl ion action), which interferes with cytoplasmic membrane integrity [34].…”

Section: Discussionsupporting

confidence: 92%

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Cytotoxicity of QMix™ endodontic irrigating solution on human bone marrow mesenchymal stem cells

et al. 2014

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BackgroundDebridement and disinfection of the root canal system is a crucial step in endodontic procedures. The effectiveness of irrigation relies on both the mechanical flushing action and the ability of irrigants to dissolve tissue and kill bacteria. The objective of the present study is to evaluate and compare the cytotoxicity of QMix™ root canal irrigating solution on immortalized human bone marrow mesenchymal stem cells (hTERT-MSC-C1) and to compare it with that of sodium hypochlorite (NaOCl).MethodsImmortalized human bone marrow mesenchymal stem cells (hTERT-MSCs) were exposed to QMix™ and NaOCl. Cell viability was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and alamarBlue assays. The cell morphology was studied after two hours of exposure to QMix™ and NaOCl. Scanning electron microscopy (SEM) analyses were performed after 2- and 4-hour incubation periods. Finally, ethidium bromide/acridine orange (EB/AO) fluorescent stain was applied to the cells in the 8-chamber slides after they were incubated with the testing agents for 2 hours to detect live and dead cells. The observations were tabulated and analyzed statistically.ResultsQMix™ exposure resulted in a significantly higher percentage of cell viability than NaOCl in the MTT and alamarBlue assays at three time points compared to the control. The SEM analysis demonstrated minimal morphological changes associated with cells that were exposed to the QMix™ solution, with little shrinkage and fragmentation of the cell wall. The live/dead analysis showed that the number of live cells after exposure to QMix™ was similar to that of the untreated control. No cell structure could be observed with the NaOCl group, indicating cell lysis.ConclusionBoth the QMix™ and NaOCl solutions were toxic to human bone marrow MSCs. Each solution might have induced cell death in a different way as evidenced in the cell viability, SEM and fluorescent studies. The slower cell death induced by QMix™ might therefore be less aggressive and more acceptable to living tissues.

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Section: Discussionsupporting

confidence: 92%

“…The protocol of Simbula et al [23] was used in this study, with some modifications. The human bone marrow mesenchymal stem cells were provided by Professor Moustapha Kassem at the Odense University Hospital, Denmark [24].…”

Section: Methodsmentioning

confidence: 99%

Cytotoxicity of QMix™ endodontic irrigating solution on human bone marrow mesenchymal stem cells

et al. 2014

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“…The cytotoxicity of EDTA was comparable with the findings of one study (Serper et al 2001). However, the IC 50 values found in the present study are not in agreement with the results of other studies (Heling et al 2001, Zhang et al 2003, Simbula et al 2010, Yasuda et al 2010, Marins et al 2012, AlKahtani et al 2014). The different cell line chosen and assay method used may be responsible for the different results.…”

Section: Discussioncontrasting

confidence: 55%

Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine

et al. 2015

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“…Cell-based cytotoxic tests are a widely accepted methodology for testing dental materials' cytotoxicity, with fibroblast cells being a convenient and reproducible model, as listed by ISO/EN10993-5 [13,14,24]. In addition to the well-known role of fibroblasts on collagen synthesis central to the extracellular matrix of the pulp tissue, these cells are fundamental for several other functions, including stem cell recruitment and differentiation, angiogenesis, and nerve sprouting and regeneration.…”

Section: Discussionmentioning

confidence: 99%

“…Human fibroblasts were used to access whitening product cytotoxicity [13,14]. Cells were obtained according to ethical and legislation protocols [15] and maintained at 37 • C in a humidified atmosphere with 95% air and 5% CO 2 in a cell incubator (Heraeus HeraCell 150 CO 2 -BridgePath Scientific, MD, USA).…”

Section: Cell Culturementioning

confidence: 99%

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

et al. 2020

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Tooth whitening procedures are increasing; however, side effects can occur, such as damage to pulp cells, by the whitening products. This study aims to assess the cellular effects promoted by a whitening product, namely, the oxidative stress fostered by the active agent hydrogen peroxide, with and without photoactivation. Additionally, if cellular recovery occurred, we intended to determine the time point where cells recover from the tooth whitening induced damage. Human fibroblasts were exposed to hydrogen peroxide, Zoom®, Zoom® + irradiation, and irradiation alone. The following analysis was performed: metabolic activity evaluation by the MTT assay; cell viability, mitochondrial membrane potential, peroxides production, superoxide radical production, and reduced glutathione expression by flow cytometry. We determined the IC50 value for all groups, and a dose-dependent cytotoxic effect was verified. At the times analyzed, hydrogen peroxide groups showed no metabolic activity recovery while a cell recovery was observed after 24 h (Zoom®) and 48 h (Zoom® + irradiation). Cell death was seen in hydrogen peroxide and Zoom® + irradiation groups, mainly by apoptosis, and the irradiation had a cytotoxic effect per se. This in vitro study supports that whitening products with moderate hydrogen peroxide (HP) concentration have a temporary effect on cells, allowing a cellular recovery.

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Comparison of Tetraacetylethylendiamine + Sodium Perborate and Sodium Hypochlorite Cytotoxicity on L929 Fibroblasts

Cited by 23 publications

References 17 publications

Cytotoxicity of QMix™ endodontic irrigating solution on human bone marrow mesenchymal stem cells

Cytotoxicity of QMix™ endodontic irrigating solution on human bone marrow mesenchymal stem cells

Combined and independent cytotoxicity of sodium hypochlorite, ethylenediaminetetraacetic acid and chlorhexidine

Cytotoxic Effects of Zoom® Whitening Product in Human Fibroblasts

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