Comparison of Systems for Identification and Differentiation of Species within the Genus
            <i>Yersinia</i>

Neubauer, Heinrich; Sauer, Thomas; Becker, H.; Aleksić, S.; Meyer, Hermann

doi:10.1128/jcm.36.11.3366-3368.1998

Cited by 36 publications

(13 citation statements)

References 18 publications

(17 reference statements)

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“…Yersinia spp. provoke false or doubtful identi¢cation in most commercial biochemical identi¢ca-tion systems [26]. Conventional polymerase chain reaction (PCR) methods for detecting Y. pestis have been developed [7,16,24,27,35].…”

Section: Introductionmentioning

confidence: 99%

Rapid detection ofYersinia pestiswith multiplex real-time PCR assays using fluorescent hybridisation probes

Tomaso

Reisinger

Dahouk³

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

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The objective of the present study was to establish a system of real-time polymerase chain reactions (PCRs) for the specific detection of Yersinia pestis using the LightCycler (LC) instrument. Twenty-five strains of Y. pestis, 94 strains of other Yersinia species and 33 clinically relevant bacteria were investigated. Assays for the 16S rRNA gene target and the plasminogen activator gene (resides on the 9.5-kb plasmid) and for the Y. pestis murine toxin gene and the fraction 1 antigen gene (both on the 100-kb plasmid) were combined for the use in two multiplex assays including an internal amplification control detecting bacteriophage lambda-DNA. Applying these multiplex assays, Y. pestis was selectively identified; other bacteria yielded no amplification products. The lower limit of detection was approximately 0.1 genome equivalent. Rat or flea DNA had no inhibitory effects on the detection of Y. pestis. The results obtained using the multiplex real-time assays showed 100% accuracy when compared with combinations of conventional PCR assays. We developed and evaluated a highly specific real-time PCR strategy for the detection of Y. pestis, obtaining results within 3 h including DNA preparation.

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Section: Introductionmentioning

confidence: 99%

Rapid detection ofYersinia pestiswith multiplex real-time PCR assays using fluorescent hybridisation probes

Tomaso

Reisinger

Dahouk³

et al. 2003

FEMS Immunology &Amp; Medical Microbiology

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“…It is likely that failure to detect Y. enterocolitica-like strains involved in the pathogenesis of human diseases is attributed to their biochemical similarity and to the low number of discriminating features in comparison with the biochemical pro¢les of Y. enterocolitica. Many commercially available identi¢cation systems are unable to identify Y. enterocolitica-like strains to the species level, because [16,17].…”

Section: Introductionmentioning

confidence: 99%

Natural antimicrobial susceptibilities and biochemical profiles ofYersinia enterocolitica-like strains:Y. frederiksenii,Y. intermedia,Y. kristenseniiandY. rohdei

Stock

Wiedemann

2003

FEMS Immunology &Amp; Medical Microbiology

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The natural susceptibility of 131 Yersinia strains of Y. frederiksenii (n=38), Y. intermedia (n=48), Y. kristensenii (n=26) and Y. rohdei (n=19) to 70 antibiotics was tested. Minimum inhibitory concentration (MIC) values were determined with a microdilution procedure in IsoSensitest broth (all strains) and cation-adjusted Mueller Hinton broth (some strains). All species were naturally sensitive or sensitive and of intermediate susceptibility to tetracyclines, aminoglycosides, acylureidopenicillins, numerous cephalosporins, carbapenems, aztreonam, quinolones, chloramphenicol, folate-pathway inhibitors, nitrofurantoin, and fosfomycin. Uniform natural resistance was found with penicillin G, oxacillin, several macrolides, lincosamides, streptogramins, glycopeptides, rifampicin and fusidic acid. Species-specific differences in susceptibility affecting clinical assessment criteria were seen with aminopenicillins (in the presence and absence of beta-lactamase inhibitors), ticarcillin and some cephalosporins. Major medium-dependent susceptibilities were found with fosfomycin. beta-Lactam MIC susceptibility patterns suggested that most strains of the species tested produce both class A and class C (AmpC) beta-lactamases that are characteristic for the species. The present study describes a database concerning the natural susceptibility of some Y. enterocolitica-like species to a wide range of antibiotics, which can be applied to validate forthcoming antibiotic susceptibility tests of these strains and might contribute to their identification. An evaluation of 30 biochemical tests that secured phenotypic identification to the Yersinia species level is presented.

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“…The species identification of these colonies with the API 20E system resulted in seven Aeromonas spp., eight Citrobacter spp., two Enterobacter spp., two Pantoea spp., one Pseudomonas aeruginosa , 14 Serratia spp., 17 Providencia retgeri and one Y. enterocolitica . Until now, the API system is still accepted as the ‘gold standard’ (Izard et al., 1984; Overmann et al., 1985; Neubauer et al., 1998) to which the Yersinia identification kit and the 16S rRNA based PCR assay have to be compared. All identification systems used in this survey identified the Y. enterocolitica strain correctly.…”

Section: Resultsmentioning

confidence: 99%

Identification of Yersinia enterocolitica in Minced Meat: A Comparative Analysis of API 20E, Yersinia Identification Kit and a 16S rRNA‐based PCR Method

Arnold

Neubauer²,

Νικολάου³

et al. 2004

Journal of Veterinary Medicine, Series B

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The isolation and identification of Yersinia enterocolitica from minced meat on CIN agar medium is still one of the major problems in food microbiology because of the low selectivity of cefsulodin-irgasan-novobiocin (CIN) agar. A total of 198 minced meat samples were collected from commercial establishments (butcher shops and supermarkets) in seven German cities in order to investigate the sensitivity and specificity of three identification techniques suitable for the differentiation of Y. enterocolitica within the rich background flora on CIN agar plates. As expected isolation of Y. enterocolitica from minced meat on CIN agar medium after 72 h enrichment in peptone, sorbitol and bile salts (PSB) broth was difficult because all plates were abundantly covered with numerous 'typical'Yersinia-like colonies of bull's eye appearance as well as with atypical colonies. Based on the phenotype of the colonies it was possible to detect colonies showing Yersinia-like growth on CIN agar in 52 samples (26%). For identification of Y. enterocolitica the API 20E system (bioMerieux, Nürtingen, Germany), the Yersinia identification kit (Merlin, Bornheim-Hersel, Germany) and a 16S rRNA based PCR assay were compared. Only in one sample (0.5%) a Y. enterocolitica strain was detected by all methods. Of the three identification systems tested for routine laboratory diagnostics the API 20E system was found to be the most suitable tool to identify Y. enterocolitica colonies within the rich background flora from minced meat samples on CIN agar plates.

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Comparison of Systems for Identification and Differentiation of Species within the Genus Yersinia

Cited by 36 publications

References 18 publications

Rapid detection ofYersinia pestiswith multiplex real-time PCR assays using fluorescent hybridisation probes

Rapid detection ofYersinia pestiswith multiplex real-time PCR assays using fluorescent hybridisation probes

Natural antimicrobial susceptibilities and biochemical profiles ofYersinia enterocolitica-like strains:Y. frederiksenii,Y. intermedia,Y. kristenseniiandY. rohdei

Identification of Yersinia enterocolitica in Minced Meat: A Comparative Analysis of API 20E, Yersinia Identification Kit and a 16S rRNA‐based PCR Method

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