Comparison of supercritical fluid and solvent extraction methods in extracting γ‐oryzanol from rice bran

Xu, Zhimin; Godber, John

doi:10.1007/s11746-000-0087-4

Cited by 123 publications

(64 citation statements)

References 13 publications

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“…It was carried out in according to the method described by Xu and Godber (2000). Table 2 shows the values of the coded and real levels of the design.…”

Section: Experimental Design To Study the Extraction Conditions Of γ mentioning

confidence: 99%

“…γ -oryzanol is mainly composed of trans-ferulic acid esters (trans-hydroxycinnamic acid) and phytosterols (sterols and triterpenic alcohols) such as cycloartenol, β -sitosterol, 24-methylenecycloartenol, and campesterol (XU;GODBER, 2000;LERMA-GARCÍA et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The methods reported for the extraction of γ -oryzanol include liquid-liquid phase extraction, solid phase extraction, supercritical fluid extraction, and direct solvent extraction (XU; GODBER, 2000), which has been the most commonly used since it does not require any specific extraction instrumentation. These 4 methods involve repeated extractions of rice bran oil (RBO), generally with hexane.…”

Section: Introductionmentioning

confidence: 99%

“…These 4 methods involve repeated extractions of rice bran oil (RBO), generally with hexane. Under optimal extraction conditions, however, th e most polar solvents, hexane:isopropanol (1:1 v/v), extracted more γ -oryzanol from rice bran than hexane at high temperature, even though hexane recovered more RBO (HU et al, 1996;XU;GODBER, 2000;SAENJUM et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Extraction of γ-oryzanol from rice bran

2012

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ABSTRACTγ -oryzanol, a phytochemical, has antioxidant activities and potential health benefits. In this study, we aimed to develop a method for extracting high amounts of γ -oryzanol from rice bran and for verifying its stability in different solvents. Of the 5 methods under investigation, the one that yielded the highest γ -oryzanol content was further improved by using a central composite rotational design (DCCR -2 4 , including 8 tests in axial conditions and 4 central points, totaling 28 trials). The parameters under study were temperature, time, volume, and the ratio of hexane:isopropanol. High γ -oryzanol content (13.98 mg . g -1 ) was obtained by using the following extraction conditions: 40° C, 40 min, and 75 mL hexane:isopropanol (1:3). The limits of detection and quantification of the method were 0.9 µg g -1 and 31 µg g -1 , respectively. The instrumental precision was 0.004%, the repeatability (CV r ) was 9.4%, and the recovery was 111.7 ± 17.7%. γ -oryzanol is more stable in isopropanol than in hexane, in which 100% concentration could be maintained after refrigeration for 72 days.Index terms: Antioxidant, spectrophotometer, stability, phytochemistry. RESUMOγ -orizanol, um fitoquímico, tem atividade antioxidante e benefícios potenciais para saúde. Esse estudo objetivou desenvolver um método para extração de alta quantidade de γ -orizanol de farelo de arroz e verificar sua estabilidade em diferentes solventes. Dentre os cinco métodos estudados, o que teve maior rendimento em γ -orizanol foi melhorado, usando um delineamento composto central rotacional (DCCR -2 4 , incluindo 8 experimentos no ponto axial e 4 no ponto central, totalizando 28 experimentos). As variáveis testadas foram temperatura, tempo, volume e proporção de hexano: isopropanol usados na extração. Elevado conteúdo de γ -orizanol (13,98 mg g -1 ) foi obtido quando as condições de extração foram 40° C, 40 min e 75 mL de hexano:isopropanol (1:3). Os limites de detecção e quantificação do método foram 0,9 µg g -1 e 31,0 µg g -1 , respectivamente. A precisão instrumental foi 0,004%, a repetibilidade (CV r ) 9,4% e a recuperação 111,7 ± 17,7%. γ -orizanol é mais estável em isopropanol do que em hexano, onde 100% da concentração foi mantida após refrigeração por 72 dias.Termos para indexação: Antioxidante, espectrofotômetro, estabilidade, fitoquímica.

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“…It was carried out in according to the method described by Xu and Godber (2000). Table 2 shows the values of the coded and real levels of the design.…”

Section: Experimental Design To Study the Extraction Conditions Of γ mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Extraction of γ-oryzanol from rice bran

2012

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“…Hexane is relatively simple and excellent for extracting nonpolar lipids. 23) However, it is highly volatile and is considered toxic to animal and humans at relatively low concentrations. In addition, removing residual hexane is expensive and time consuming.…”

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confidence: 99%

<i>In Vivo</i> Hair Growth-Promoting Effect of Rice Bran Extract Prepared by Supercritical Carbon Dioxide Fluid

Choi

Jeon²,

Moon³

et al. 2014

Biological & Pharmaceutical Bulletin

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The potential hair growth-promoting activity of rice bran supercritical CO 2 extract (RB-SCE) and major components of RB-SCE, linoleic acid, policosanol, γ-oryzanol, and γ-tocotrienol, were evaluated with the histological morphology and mRNA expression levels of cell growth factors using real-time reverse transcriptase-polymerase chain reaction (PCR) in C57BL/6 mice. RB-SCE showed hair growth-promoting potential to a similar extent as 3% minoxidil, showing that the hair follicles were induced to be in the anagen stage. The numbers of the hair follicles were significantly increased. In addition, mRNA expression levels of vascular endothelial growth factor (VEGF), insulin-like growth factor-1 (IGF-1), and keratinocyte growth factor (KGF) were also significantly increased and that of transforming growth factor-β (TGF-β) decreased in RB-SCE-treated groups. Among the major components of RB-SCE, linoleic acid and γ-oryzanol induced the formation of hair follicles according to examination of histological morphology and mRNA expression levels of cell growth factors. In conclusion, our results demonstrate that RB-SCE, particularly linoleic acid and γ-oryzanol, promotes hair growth and suggests RB-SCE can be applied as hair loss treatment. Key words rice bran supercritical CO 2 extract; hair growth-promoting activity; in vivoAlthough hair loss is not a mortal disorder, it has a great impact on a person's self-respect, mental health, and overall quality of life. Approximately 50-60 hairs are normally lost per day, which does not have a noticeable effect on appearance; however, excess loss (>100) results in baldness. Androgenetic alopecia is the most common type of hair loss, affecting millions of both men and women.1) There are many causes of hair loss in men and women, including diseases, nutritional deficiency, aging, hormone imbalance, and stress. Androgenetic alopecia may occur as early as the teenage years, but typically begin in the later decades of life. 2,3) Hair loss affects at least half of all men by the age of 50 and up to 70% of 70-year-old men. 4)

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Analysis Methods of Phenolic Acids

2012

Analysis of Antioxidant‐Rich Phytochemicals

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Comparison of supercritical fluid and solvent extraction methods in extracting γ‐oryzanol from rice bran

Cited by 123 publications

References 13 publications

Extraction of γ-oryzanol from rice bran

Extraction of γ-oryzanol from rice bran

<i>In Vivo</i> Hair Growth-Promoting Effect of Rice Bran Extract Prepared by Supercritical Carbon Dioxide Fluid

Analysis Methods of Phenolic Acids

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