Comparison of structural proteins among antigenically different Japanese encephalitis virus strains

Hasegawa, Hitoshi; Yoshida, Masamichi; Fujita, Shigeru; Kobayashi, Yoshinari

doi:10.1016/0264-410x(94)90294-1

Cited by 56 publications

(49 citation statements)

References 24 publications

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“…gene region, 9 while a fifth genotype surfaced in our analysis, containing the lone Singapore Muar isolate. 41 The Muar strain was the most divergent of the JEV isolates analyzed, differing from genotypes I, II, III, and IV by a minimum nucleotide divergence of 21%, 20%, 19.3%, and 22% respectively. Using a 7% cut-off value of nucleotide divergence, the Muar strain could clearly be placed in a newly formed fifth genotype.…”

Section: Resultsmentioning

confidence: 90%

Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent.

Uchil¹,

Satchidanandam²

2001

The American Journal of Tropical Medicine and Hygiene

150

108

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Abstract. We report the analysis of the complete nucleotide sequence for the Indian isolate (P20778; Genbank Accession number AF080251) of Japanese encephalitis virus (JEV). The phylogenetic tree topology obtained using thirteen complete genome sequences of JEV was reproduced with the envelope, NS1, NS3, and NS5 genes and revealed extensive divergence between the two Indian strains included. A more exhaustive analysis of JEV evolution using 107 envelope sequences available for isolates from different geographic locations worldwide revealed five distinct genotypes of JEV, displaying a minimum nucleotide divergence of 7% with high bootstrap support values. The tree also revealed overall clustering of strains based on geographic location, as well as multiple introductions of JEV into the Indian subcontinent. Nonsynonymous nucleotide divergence rates of the envelope gene estimated that the ancestor common to all JEV genotypes arose within the last three hundred years.

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Section: Resultsmentioning

confidence: 90%

Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent.

Uchil¹,

Satchidanandam²

2001

The American Journal of Tropical Medicine and Hygiene

150

108

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“…The prM nucleotide sequence information of 56 JE strains was obtained from previous publications (9,10). The nucleotide sequence data of the E protein genes of Nakayama, Beijing-1, Kamiyama, 691004 and Muar were obtained from Hasegawa et al (18) and ThCMAr4492 and ThCMAr6793 from our previous report (1). The secondary structure and hydrophobicity of the protein were predicted by Chou and Fasman (11) and Hoop and Woods (25) methods, respectively.…”

Section: Two Primers Je317smentioning

confidence: 99%

Antigenic and Genetic Variations among Japanese Encephalitis Virus Strains Belonging to Genotype 1

Ali

Igarashi

1997

Microbiology and Immunology

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Hyperimmune antisera against four Japanese encephalitis (JE) virus strains, ThCMAr4492 and ThCMAr6793 from Thailand and Nakayama and JaGAr01 from Japan, were used to analyze the antigenic relationships among 12 Thai strains belonging to genotype 1, and two Japanese strains and one Chinese strain belonging to genotype 3. The antiserum for ThCMAr6793 significantly neutralized nine of the 12 Thai strains, none of which was significantly neutralized by antisera for the Nakayama and JaGAr01 strains. The antiserum for ThCMAr4492 neutralized only its homologous strain; therefore, ThCMAr4492 was antigenically different from all other strains. Two Thai strains (Subin and KE-093/83) were significantly less neutralized by all four of the antisera tested. In the deduced amino-acid sequence of the E protein, the 12 Thai strains revealed 100 to 98.2 % identity among them and 90.0 to 98.8 % identity with the published strains, respectively. Among significant amino-acid substitutions, three residues at positions E-222, E-327 and E-366 were shared by all of the Thai strains, whereas residues at E-89, E-123, E-131, E-178, E-293, E-351 and E-373 seemed to be strain-specific. The amino acids at positions E-178, E-327, E-351, E-373 and E-366 are found either in the peptides with functional T-helper cell epitopes or in the ectodomain of the E protein of other flaviviruses. These amino acids may therefore be responsible for determining the antigenic heterogeneity of these strains.

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“…A GV strain of JEV was first isolated from an encephalitis patient in Malaysia in 1952 (also referred to as the Muar strain), and it remained the only known instance of this genotype for over 50 years (Hasegawa et al, 1994;Solomon et al, 2003). Recently, the GV JEV genome has been identified in Culex mosquitoes in China in 2009 and in South Korea in 2010 and 2012 (Kim et al, 2015;Takhampunya et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

In vitro growth, pathogenicity and serological characteristics of the Japanese encephalitis virus genotype V Muar strain

Tajima

Yagasaki

Kotaki

et al. 2015

Journal of General Virology

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The characteristics of genotype V Japanese encephalitis virus (GV JEV) remain poorly understood as only two strains have been isolated to date. In this study, we examined the effects of the GV JEV Muar strain on in vitro growth and pathogenicity in mice; we also evaluated the efficacy of inactivated JEV vaccines against the Muar strain. Although growth of the Muar strain in mouse neuroblastoma N18 cells was clearly worse than that of the GIII Beijing-1 and GI Mie/41/2002 strains, neuroinvasiveness of the Muar strain was similar to that of the Beijing-1 strain and significantly higher than that of the Mie/41/2002 strain. The results of a plaque reduction neutralization test suggested that the neutralization ability of the JEV vaccines against the Muar strain was reduced compared with the GI and GIII strains. However, the protection potency of the JEV vaccine against the Muar strain was similar to that for the Beijing-1 strain in mice. Our data indicate that GV JEV has unique growth, virulence and antigenicity features.

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Comparison of structural proteins among antigenically different Japanese encephalitis virus strains

Cited by 56 publications

References 24 publications

Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent.

Phylogenetic analysis of Japanese encephalitis virus: envelope gene based analysis reveals a fifth genotype, geographic clustering, and multiple introductions of the virus into the Indian subcontinent.

Antigenic and Genetic Variations among Japanese Encephalitis Virus Strains Belonging to Genotype 1

In vitro growth, pathogenicity and serological characteristics of the Japanese encephalitis virus genotype V Muar strain

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