Comparison of structural integrity and functional status of corneal endothelium stored in Cornisol and Optisol-GS

Sundaresan, Yogapriya; Gaikwad, Ganesh Govardhan; Prajapati, Kishan Anilkumar; Prajna, Namperumalsamy Venkatesh; Chidambaranathan, Gowri Priya

doi:10.4103/ijo.ijo_2026_18

Cited by 11 publications

(4 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, pH of the medium which is a critical physiological parameter for effective maintenance of mammalian cells (Michl et al 2019 ; Kisaka et al 2015 ) was not measured before and after storage. Optisol has a baseline pH of 7.4 while it ranges from 6.46 to 7.33 after storage of corneal buttons for a period of 7–14 days (Sundaresan et al 2019 ) MK medium has a baseline pH of 7.5 while it is around 8.2 after storage of corneal buttons for a period of 4 days (Hasany and Basu 1987 ). pH of TGP is neutral.…”

Section: Discussionmentioning

confidence: 99%

A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

Namitha¹,

Chitra²,

Bhavya³

et al. 2021

Biotechnol Lett

View full text Add to dashboard Cite

Purpose McCarey-Kaufman’s (MK) medium and Optisol-GS medium are the most commonly employed media for human donor corneal preservation. In this study, we evaluated the preservation efficacy of discarded human donor corneas using a Thermo-reversible gelation polymer (TGP) added to these two media. Methods Thirteen human corneal buttons collected from deceased donors, which were otherwise discarded due to low endothelial cell density (ECD) were used. They were stored in four groups: MK medium, MK medium with TGP, Optisol-GS and Optisol-GS with TGP at 4 °C for 96 h. Slit lamp examination and specular microscopy were performed. Corneal limbal tissues from these corneas were then cultured using explant methodology one with and the other without TGP scaffold, for 21 days. Results MK + TGP and Optisol-GS + TGP preserved corneas better than without TGP, which was observed by maintenance of ECD which was significantly higher in Optisol-GS + TGP than MK + TGP (p-value = 0.000478) and corneal thickness remaining the same for 96 h. Viable corneal epithelial cells could be grown from the corneas stored only in MK + TGP and Optisol-GS + TGP. During culture, the TGP scaffold helped maintain the native epithelial phenotype and progenitor/stem cell growth was confirmed by RT-PCR characterization. Conclusion TGP reconstituted with MK and Optisol—GS media yields better preservation of human corneal buttons in terms of relatively higher ECD maintenance and better in vitro culture outcome of corneal limbal tissue. This method has the potential to become a standard donor corneal transportation-preservation methodology and it can also be extended to other tissue or organ transportation upon further validation.

show abstract

Section: Discussionmentioning

confidence: 99%

A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

Namitha¹,

Chitra²,

Bhavya³

et al. 2021

Biotechnol Lett

View full text Add to dashboard Cite

show abstract

“…The staining patterns for these markers were comparable and translated to morphological changes on the tissue stored in both storage solution. 33…”

Section: Corneal Storage Solutions—types and Compositionmentioning

confidence: 99%

Overview of Short-Term and Intermediate-Term Corneal Storage Solution: Comparison of Clinical Outcomes and Need For Future Research

Roy

Jeng

Chaurasia

et al. 2022

Eye &Amp; Contact Lens: Science &Amp; Clinical Practice

View full text Add to dashboard Cite

Corneal donor tissue preservation techniques have incrementally improved since the introduction of McCarey-Kaufman storage solution from short-term storage to intermediate duration of storage with the advent of organ culture and Optisol GS storage solutions. Improved understanding of the corneal endothelial cell physiology has helped in designing newer storage solutions, such as the Life 4C and Cornea Cold. The incorporation of antibiotics, ATP precursors, minerals, and vitamins has improved the viability of tissues. In addition, these modifications to the newer storage solutions have increased the endothelial longevity and metabolic activity. Despite these advances, the duration of tissue storage has largely been restricted to 2 weeks in Optisol GS and 4 weeks in organ culture. The role and cost-effectiveness of antifungal supplementation and the need for improved epithelial preservation are additional areas that need to be explored. This review intends to summarize the efficacy and viability of donor corneas in different tissue storage solution and compare clinical outcomes while providing an insight into the challenges in developing newer methods of corneal preservation.

show abstract

“…There is a natural loss of endothelial cells in hypothermic corneal storage media, ranging from 3-9% at 7 days and 15-20% at 14 days according to various studies [7][8][9] .…”

Section: Introductionmentioning

confidence: 99%

Endothelial cell viability comparing Eusol-C vs Optisol-GS as corneal preservation medium

Mena-Linares,

Vázquez-Maya,

García-Carmona

et al. 2023

HGMX

View full text Add to dashboard Cite

Introduction:Optisol-GS is the most widely used corneal preservation medium worldwide, there is little evidence to compare it against Eusol-C as a corneal preservation medium. Objectives: To evaluate the viability of endothelial cells between two different corneal preservation media. Materials and Methods: It is a prospective, longitudinal, and comparative study, which was carried out in the eye bank of Hospital General de México "Dr. Eduardo Liceaga", from where the corneas of suitable donors were collected, the right cornea was preserved in Optisol-GS and the left in Eusol-C. Two evaluations were carried out on day 1 and 5. Endothelial cell density, coefficient of cell variation and hexagonality were analysed. Results: The decrease in endothelial cells on day 5 after collection of the corneoscleral tissue was 8.149% in the corneas stored in Optisol-GS and 7.614% in Eusol-C p=0.006. Cell variation increased 1.634% in . Conclusions: No superiority was found when comparing these two media, which shows that Eusol-C is as effective as Optisol-GS for corneal preservation for at least five days.

show abstract

Comparison of structural integrity and functional status of corneal endothelium stored in Cornisol and Optisol-GS

Cited by 11 publications

References 15 publications

A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

A novel human donor cornea preservation cocktail incorporating a thermo-reversible gelation polymer (TGP), enhancing the corneal endothelial cell density maintenance and explant culture of corneal limbal cells

Overview of Short-Term and Intermediate-Term Corneal Storage Solution: Comparison of Clinical Outcomes and Need For Future Research

Endothelial cell viability comparing Eusol-C vs Optisol-GS as corneal preservation medium

Contact Info

Product

Resources

About