Comparison of Storage Methods for Microfluidically Produced Water‐in‐Oil Droplets

Grösche, Maximilian; Korvink, Jan G.; Rabe, Kersten S.; Niemeyer, Christof M.

doi:10.1002/ceat.201900075

Cited by 3 publications

(4 citation statements)

References 31 publications

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“…S3a and S3b †), as previously established in our laboratory. 24,44,45 Furthermore, the PDMS microchannels were rendered uorophilic by coating them with per-uorooctyltrichlorosilane before use (see the Experimental section). W/FO droplets formed with the three different surfactants used in this work could be collected and stored in a vial for several days at either RT or 37 °C, indicating comparably high stability (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Charge controlled interactions between DNA-modified silica nanoparticles and fluorosurfactants in microfluidic water-in-oil droplets

et al. 2023

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Section: Resultsmentioning

confidence: 99%

“…PMMA molds were prepared using the micro-milling technique according to a previously described technique. 44 …”

Section: Methodsmentioning

confidence: 99%

Charge controlled interactions between DNA-modified silica nanoparticles and fluorosurfactants in microfluidic water-in-oil droplets

et al. 2023

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“…These results proved that, when operating at medium velocity regimes, the droplet generation process could be scaled upon maintaining a high droplet quality and homogeneity. According to the literature, bulk storage and transfer of droplets might affect the monodispersity of a droplet population in the long term; therefore, these results could be further improved by on-chip storage in fluidic microcavities [ 43 ].…”

Section: Resultsmentioning

confidence: 99%

Silicon-Based 3D Microfluidics for Parallelization of Droplet Generation

et al. 2023

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Both the diversity and complexity of microfluidic systems have experienced a tremendous progress over the last decades, enabled by new materials, novel device concepts and innovative fabrication routes. In particular the subfield of high-throughput screening, used for biochemical, genetic and pharmacological samples, has extensively emerged from developments in droplet microfluidics. More recently, new 3D device architectures enabled either by stacking layers of PDMS or by direct 3D-printing have gained enormous attention for applications in chemical synthesis or biomedical assays. While the first microfluidic devices were based on silicon and glass structures, those materials have not yet been significantly expanded towards 3D despite their high chemical compatibility, mechanical strength or mass-production potential. In our work, we present a generic fabrication route based on the implementation of vertical vias and a redistribution layer to create glass–silicon–glass 3D microfluidic structures. It is used to build different droplet-generating devices with several flow-focusing junctions in parallel, all fed from a single source. We study the effect of having several of these junctions in parallel by varying the flow conditions of both the continuous and the dispersed phases. We demonstrate that the generic concept enables an upscaling in the production rate by increasing the number of droplet generators per device without sacrificing the monodispersity of the droplets.

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“…By controlling the flowrates of the aqueous and continuous phase, highly monodispersed W/O droplets of about 180–200 μm diameter were generated with the microfluidic flow‐focusing chip. For further analysis, the generated SiNP/DOTAP droplets were then stored in an on‐chip storage chamber (OCS) …”

Section: Resultsmentioning

confidence: 99%

Segregation of Dispersed Silica Nanoparticles in Microfluidic Water‐in‐Oil Droplets: A Kinetic Study

et al. 2020

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Dispersed negatively charged silica nanoparticles segregate inside microfluidic water-in-oil (W/O) droplets that are coated with a positively charged lipid shell. We report a methodology for the quantitative analysis of this self-assembly process. By using real-time fluorescence microscopy and automated analysis of the recorded images, kinetic data are obtained that characterize the electrostatically-driven self-assembly. We demonstrate that the segregation rates can be controlled by the installment of functional moieties on the nanoparticle's surface, such as nucleic acid and protein molecules. We anticipate that our method enables the quantitative and systematic investigation of the segregation of (bio)functionalized nanoparticles in microfluidic droplets. This could lead to complex supramolecular architectures on the inner surface of micrometer-sized hollow spheres, which might be used, for example, as cell containers for applications in the life sciences.

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Comparison of Storage Methods for Microfluidically Produced Water‐in‐Oil Droplets

Cited by 3 publications

References 31 publications

Charge controlled interactions between DNA-modified silica nanoparticles and fluorosurfactants in microfluidic water-in-oil droplets

Charge controlled interactions between DNA-modified silica nanoparticles and fluorosurfactants in microfluidic water-in-oil droplets

Silicon-Based 3D Microfluidics for Parallelization of Droplet Generation

Segregation of Dispersed Silica Nanoparticles in Microfluidic Water‐in‐Oil Droplets: A Kinetic Study

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