Comparison of sperm lipid components among four species of sea-urchin based on echinoid phylogeny

Mita, Masatoshi; Oguchi, Atsuko; Kikuyama, Sakaé; Namiki, Hideo; Yasumasu, Ikuo; Nakamura, Masaru

doi:10.1016/0305-0491(94)90093-0

Cited by 8 publications

(6 citation statements)

References 6 publications

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“…Triglycerides, important for embryos during early development, are the main non-polar lipids in the eggs of sea urchins (Kozhina et al 1978;Sewell 2005;Villinski et al 2002;Yasumasu et al 1984) but are present at very low levels or even absent in spermatozoa where phospholipids clearly predominate (Kozhina et al 1978;Mita et al 1994). Triglycerides and phospholipids have usually distinctive fatty acid proWles and, for example, in the gametes of diVerent sea urchin species, it has been shown that phospholipids have higher levels of 18:0, 20:4n-6 and 20:5n-3 than triglycerides but lower proportions of 14:0, 16:0 and 16:1n-7 (Kozhina et al 1978;Metzman et al 1978;Mita et al 1994). In our study, sampling was carried out in the period of the reproductive cycle in which we knew that gametes (premature or fully developed) were present, avoiding the post-spawning or recovery stages (Martínez et al 2003;Sánchez-España et al 2004, personal observations).…”

Section: Discussionmentioning

confidence: 99%

Males and females gonad fatty acids of the sea urchins Paracentrotus lividus and Arbacia lixula (Echinodermata)

2009

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The aim of this study was to analyze male and female gonad fatty acids of two sea urchin species, Paracentrotus lividus and Arbacia lixula, from the south coast of Spain. Additionally, we investigated possible diVerences between two locations. The ovaries of both species showed higher percentages of 14:0, 16:0, 16:1n-7, 18:2n-6, 18:3n-3 and 18:4n-3 than testes and lower levels of 18:0, 22:1n-9, 20:4n-6 and 22:5n-3. In P. lividus but not in A. lixula, the level of 20:5n-3 was higher in testes than in ovaries. These diVerences between sexes probably indicate diVerent requirements of males and females during gametogenesis although the presence of a large number of gametes in the mature gonad may also have inXuences on fatty acid composition. SigniWcant diVerences in gonad fatty acid proWles where also found when individuals of P. lividus collected at a location of the Mediterranean region were compared with specimens collected at the Atlantic coast. The most remarkable changes were the lower levels of 14:0, 18:1n-7, 20:1n-9, 20:4n-6 and 22:4n-6 and the higher values of 20:1n-11, 20:5n-3 and 22:6n-3 found in males and females of the Mediterranean specimens compared to those of the Atlantic coast. These diVerences probably reXect the diVerences in potential food sources at each location.

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Section: Discussionmentioning

confidence: 99%

Males and females gonad fatty acids of the sea urchins Paracentrotus lividus and Arbacia lixula (Echinodermata)

2009

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“…[ 22,23 ] Further, only one paper reported the lipid components of the sperm of D. setosum in echinoid phylogeny. [ 24 ]…”

Section: Introductionmentioning

confidence: 99%

Lipids and Fatty Acids of the Gonads of Sea Urchin Diadema setosum (Echinodermata) From the Coastal Area of the Nha Trang Bay, Central Vietnam

Мурзина

Dgebuadze

Пеккоева

et al. 2021

Euro J Lipid Sci & Tech

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The sea urchin Diadema setosum is edible and desirable as food by locals in central Vietnam and a promising target for potential fishing. The lipid profiles of the gonads of the sea urchin inhabiting the coastal area in Nha Trang Bay are studied for the first time. The determination of the content of the total lipids (TL), total phospholipids (PL), monoacylglycerols (MAG), diacylglycerols (DAG), triacylglycerols (TAG), cholesterol (Chol), sterol esters, and free fatty acids (FFA) is analyzed by high-performance thin layer chromatography (HPTLC); the phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylcholine (PC), lysophosphatidylcholine (LPC), and sphingomyelin (SM) are determined by high-pressure liquid chromatography (HPLC); and the fatty acid levels of TLs are identified using gas chromatography (GC). Non-polar TAG, FFA, Chol, and DAG dominated. The content of total PL is significant. PC is abundant among PL fractions, followed by PE, PI, and PS. The TLs contain a high proportion of PUFA, mainly due to arachidonic fatty acid and eicosapentanoic fatty acid. The (n−6)/(n−3) ratio is 1.68, and the atherogenic and thrombogenic indexes of D. setosum are 1.13 and 0.73. The results can be used in the development of marine bioprospecting and methodological approaches for the creation of functional substances.

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“…Energy metabolism in sea urchin spermatozoa is an important consideration when swimming towards the egg. Spermatozoa of sea urchins of Echinoida, such as Hemicentrotus pulcherrimus (Mita and Ueta, 1988;Mita and Nakamura, 1993b) and Paracentrotus lividus (Mita et al , 1994a) use endogenous phospholipid, particularly phosphatidylcholine (PC), as a substrate for energy metabolism. PC is abundant in sperm midpieces (Mita et al , 1991), which contain several lipid bodies within mitochondria (Mita and Nakamura, 1992).…”

Section: Introductionmentioning

confidence: 99%

“…This is strongly suggestive of the PC available for energy metabolism being stored in the lipid bodies. In contrast, the spermatozoa of the orders Arbacioida, Diadematoida, and Clypeasteroida use endogenous triglyceride (TG) instead of PC (Mita, 1991;Mita et al , 1994aMita et al , , b, 1995. The midpiece of these spermatozoa contain a single mitochondrion and lipid globules, although there are no lipid bodies (Mita and Nakamura, 1993a;Mita et al ., 1994cMita et al ., , d, 1995.…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on the Energy Metabolism in Spermatozoa of Four Closely Related Species of Sea Urchins (Genus Echinometra) in Okinawa

2002

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Sea urchins of the genus Echinometra are abundant on Okinawa reef flats in southern Japan. The Okinawan Echinometra is designated into four sympatric and closely related species: A, B, C, and D (Ea, Eb, Ec, and Ed). The sperm head size and shape gradually changes to become longer and more slender according to the following order: Ea, Eb, Ec, and Ed. To obtain information regarding speciation in Okinawan Echinometra, this study examined comparatively the energy production system of spermatozoa of Ea, Eb, Ec, and Ed. All spermatozoa contained cholesterol and several kinds of phospholipids. Glycogen, glucose, and triglyceride were present at extremely low levels. After incubation in sea-water, a decrease in the level of phosphatidylcholine (PC) was observed in all spermatozoa concomitantly with activation of motility and respiration. The hydrolysis of PC correlated with the activity of phospholipase A2. Interestingly, the amount of PC consumed, the respiratory rate, and the phospholipase A2 activity in spermatozoa of Ea and Eb were approximately two-fold higher than those of Ec and Ed. Ultrastructural studies showed that lipid bodies within mitochondria were present in the midpieces of all species of spermatozoa. They became small or disappeared after incubation in seawater. Thus, the results obtained strongly suggest that spermatozoa of Ea, Eb, Ec, and Ed all use PC located in the lipid bodies as a substrate for energy metabolism. Also, it seems likely that energy production activities in Ea and Eb spermatozoa are stronger than those in Ec and Ed. The properties of energy metabolism in different species of sea urchin may be related to their habitat.

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Comparison of sperm lipid components among four species of sea-urchin based on echinoid phylogeny

Cited by 8 publications

References 6 publications

Males and females gonad fatty acids of the sea urchins Paracentrotus lividus and Arbacia lixula (Echinodermata)

Males and females gonad fatty acids of the sea urchins Paracentrotus lividus and Arbacia lixula (Echinodermata)

Lipids and Fatty Acids of the Gonads of Sea Urchin Diadema setosum (Echinodermata) From the Coastal Area of the Nha Trang Bay, Central Vietnam

Comparative Studies on the Energy Metabolism in Spermatozoa of Four Closely Related Species of Sea Urchins (Genus Echinometra) in Okinawa

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