Comparison of sperm adenosine triphosphate content, motility and fertility of immobilized and conventionally cryopreserved Norwegian Red bull semen

Berg, Halldor Felde; Kommisrud, Elisabeth; Bai, Godlove; Gaustad, Eline Rustad; Klinkenberg, Geir; Standerholen, Fride Berg; Thorkildsen, Lill Therese; Waterhouse, Karin Elisabeth; Ropstad, Erik; Heringstad, B.; Alm-Kristiansen, Anne Hege

doi:10.1016/j.theriogenology.2018.08.016

Cited by 9 publications

(10 citation statements)

References 29 publications

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“…However, due to species, its production and metabolism process is not fully understood. Some researchers have found correlation between the fertility in different bull breeds [ 50 ] and the motility in starlet [ 51 ], whereas other reports on mammals have found that there is no correlation between fertility and ATP content of sperm [ 52 , 53 ], but it is related to the motility parameters [ 52 ]. In the present study, the ATP concentration in 0.8 mg/mL group was higher than that of other groups on 5th day of semen preservation, which was corresponding to viability and PM during semen preservation and consistent with stallion [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Wang

Zhang

Sohail

et al. 2022

Animals

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The purpose of this study was to investigate whether the addition of chlorogenic acid (CGA) to a sheep semen extender could improve the quality of chilled sheep sperm. Ejaculates (n = 80) were collected from five Hu rams with an artificial vagina. The ejaculates were mixed and divided into five equal parts, diluted with a CGA-free Tris–egg yolk extender (control), or supplemented with 0.2, 0.4, 0.8, and 1.2 mg/mL. The sperm kinematic parameters (viability, progressive motility), functional integrity of plasma membrane and acrosome, adenosine triphosphate (ATP) concentration and antioxidant parameters (Catalase (CAT), Superoxide dismutase (SOD) activity, total antioxidant capacity (T-AOC), ROS level and Malondialdehyde (MDA) content) were evaluated during storage of the semen. The results indicated that: PM, plasmatic membrane integrity and acrosomal integrity in 0.8 mg/mL CGA were higher (p < 0.05) from day 1 to 5. The ROS level in CGA groups was lower than the control (p < 0.05). CAT, SOD, ATP, and T-AOC were highest at 0.8 mg/mL concentration within 1 to 5 days. The above results indicated that the right concentration of CGA improved the quality of Hu ram sperm during chilling storage.

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Section: Discussionmentioning

confidence: 99%

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Wang

Zhang

Sohail

et al. 2022

Animals

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“…The motility of sperm is the most critical parameter for evaluating male potential fertility [77], and it depends on mitochondrial function [180,181]. Motility includes total general motility, progressive motility, and kinematic parameters [181]. Sperm cells must possess high maintenance of sperm motility to ensure maximum potential fertility [114,182].…”

Section: Sperm Motilitymentioning

confidence: 99%

“…Moreover, the motility of sperm cells is also associated with sperm DNA defects. Furthermore, sperm motility is crucial for fertilization [45,181]. Immotile sperm and motility disorders of sperm are notable indicators of male infertility [183].…”

Section: Sperm Motilitymentioning

confidence: 99%

“…Immotile sperm and motility disorders of sperm are notable indicators of male infertility [183]. Therefore, microscopic examination and estimation of the percentage of forward movement of sperm are a standard test to indicate male fertility [45,181].…”

Section: Sperm Motilitymentioning

confidence: 99%

“…Potential fertility depends on multiple parameters that require a multi-parametric analysis of sperm morphology, sperm motility, membrane status of sperm, sperm acrosome reaction, and genome integrity of sperm to provide a complete picture of a male's fertility potential. Moreover, the reliability of fertility prediction is reported to increase by combining several in vitro sperm quality parameters [180,181,203]. The difficulty of this assay is that it is a time-consuming and costly procedure as hundreds of successful inseminations are required.…”

Section: In Vivo Fertility Evaluationmentioning

confidence: 99%

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Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Bustani

Baiee

2021

Vet World

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Reproduction is fundamental for all living things as it ensures the continued existence of a species and an improved economy in animal husbandry. Reproduction has developed since history, and diverse processes, such as artificial insemination and in vitro fertilization, have been developed. Semen extenders were discovered and developed to protect sperm from harmful factors, such as freeze and osmotic shock, oxidative stress, and cell injury by ice crystals. Semen extenders preserve sperm by stabilizing its properties, including sperm morphology, motility, and viability and membrane, acrosomal, and DNA integrity. Therefore, semen extenders must provide a favorable pH, adenosine triphosphate, anti-cooling and anti-freeze shock, and antioxidant activity to improve semen quality for fertilization. Hence, this review provides precise data on different semen extenders, preservative mechanisms, and essential additives for semen extenders in different animals.

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Post‐thaw semen quality in young bull ejaculates before being accepted for commercial semen doses

Hurri¹,

Lima-Verde

Johannisson

et al. 2022

Veterinary Record

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Background: Genomic selection enables bulls with desirable characteristics to be identified at a young age, but sperm quality can be poor in the ejaculates of young bulls. Few studies have been done on post-thaw sperm quality in bulls less than 10 months old. The objective of this study was to determine the age at which post-thaw sperm quality was acceptable for artificial insemination. Methods: Semen was collected by artificial vagina; samples containing 100-500 million spermatozoa/ml were frozen for this study. Post-thaw analyses of membrane integrity (MI), mitochondrial membrane potential (MMP), chromatin integrity, morphology, production of reactive oxygen species and sperm kinematics were made. Results: The age at which ejaculates exceeded the breeding company's thresholds of acceptance varied considerably among individuals, with 285 days being the earliest. Morphology (p < 0.003), MI (p = 0.0096), high MMP (p = 0.043) and superoxide production (p = 0.0084) increased between the first and last ejaculates but reached acceptable levels at different ages for individual bulls. Conclusions: It was possible to obtain acceptable post-thaw sperm quality from samples even though sperm concentration was lower than the breeding company's threshold. Therefore, it might be feasible to use ejaculates earlier than is currently considered possible, by modifying semen handling protocols.

show abstract

Comparison of sperm adenosine triphosphate content, motility and fertility of immobilized and conventionally cryopreserved Norwegian Red bull semen

Cited by 9 publications

References 29 publications

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Chlorogenic Acid Improves Quality of Chilled Ram Sperm by Mitigating Oxidative Stress

Semen extenders: An evaluative overview of preservative mechanisms of semen and semen extenders

Post‐thaw semen quality in young bull ejaculates before being accepted for commercial semen doses

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