Comparison of Spectrodensitograms of the Selected Drugs on Different Chromatographic Sorbents

Pyka, Alina; Babuska, M.; Dziadek, A.; Gurak, D.

doi:10.1080/10826070701276713

Cited by 6 publications

(2 citation statements)

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“…This fact was also observed for other drugs. [10,11] It was stated that a larger amount of the bands on a chromatogram were obtained on RP8F 254s plates than on RP18F 254s plates. Obtained data indicate that vitamin K 1 had quite a few of the impurities of an unidentified structure.…”

Section: Resultsmentioning

confidence: 99%

Use of RP-TLC and Densitometry to Analytical Characteristic of Vitamin K₁

Pyka

Gurak

2009

Journal of Liquid Chromatography & Related Technologies

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Vitamin K 1 (phylloquinone) was investigated with the use of reversed phase thin layer chromatography (RP-TLC) on RP8F 254s , and RP18F 254s (E. Merck) plates, using methanol as a mobile phase. It was stated that a larger amount of the bands on a chromatogram were obtained on RP8F 254s plates than on RP18F 254s plates. Six densitometric bands on RP8F 254s plates were observed, where vitamin K 1 had R F ¼ 0.45 and k max ¼ 254 nm; however, the impurities had the R F values equal to 0.27, 0.54, 0.59, 0.62, and 0.75, respectively. It was stated that RP-TLC with densitometry and spectrodensitometry is a good method for the analysis of the qualitative composition of vitamin K 1 .

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