Comparison of some peptidic and proteic ovine pineal fractions with a bovine pineal E5 fraction

Abstract

Using rather simple and mild extraction and separation methods, three ovine pineal fractions (XM 300 R-PP7.2' and PP7.2 S) were obtained, which contain peptidic/proteic substances and which show fluorescence characteristics of indoles. The ovine fractions were compared with the bovine pineal E-5 fraction. The ovine fractions are chemically sensitive to normal laboratory light and stable in red light (lambda greater than 600 nm). Immunologically, these fractions and the bovine E 5 fraction are stable. From the results of radioimmunological experiments it was concluded that the bovine pineal E 5 fraction as well as the ovine pineal fraction XM 300 R-PP7.2 and PP7.2S may contain (a) peptide(s) ending by the same carboxy terminal tripeptide Pro-Arg-Gly(NH2).

Characterization of a Neurohypophyseal Hormone‐Like Activity Isolated From Ovine Pineal Glands

“…Repeated analysis with the AVP RIA [Dogterom et al, 19771 of pineal fractions containing milk-ejecting activity produced yields of an AVP-like immunoreactivity lower than those obtained with the RIA for Pro-Arg-Glyamide endings [Czernichow et al, 19741. This discrepancy was found to be due to the use of different AVP-( 1-9) standards and also to the presence of nonvasotocin compounds that are recognized by the anti-Pro-Arg-Gly( NH2) antiserum when the RIA is performed without a preassay chemical separation Pevet, 1982;Noteborn et al, 1982, 19871. Nevertheless, from the present experiments, there is no evidence that the observed biological activity is from a peptide with a Pro-Arg-Gly( NH2) carboxy terminal ending [Pevet et al, 19811. HPLC in conjunction with RIA failed to detect another AVP-like peptide that did not correspond to the elution position of synthetic AVP-( 1-9).…”

“…The method of extraction and prefractionation of pineal tissue was performed in batches of 50 gm (wet tissues) using an aqueous extraction followed by ultrafiltration steps as described elsewhere in detail [ Noteborn et al, 1982;(see also Fig. l)].…”

Partial Purification of a Polypeptide Extract Derived From Ovine Pineal That Suppresses the Growth of Human Melanoma Cells In Vitro

“…The method of Noteborn et al [ 1982 was used for the extraction and prefractionation of ovine pineal tissue. Frozen glands ( 5 0 gm wet weight) were cleaned from contaminations and homogenized in 50 ml distilled water using a tissue homogenizer (Waring type blender).…”