Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads

Li, Fengmin; Li, Fuyun; Hou, Xiaodong; Luo, Xianxiang; Tu, Hai-Feng; Zou, Ya-Dan; Sun, Cuizhu; Shi, Mei; Zheng, Hao

doi:10.1016/j.marpolbul.2018.04.056

Cited by 26 publications

(12 citation statements)

References 42 publications

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“…Only peaks diagnostic for PS bonds susceptible and indicative of polymer reactivity (i.e., aromatic and aliphatic bonds) were monitored. [1,17,26,28] PL was used to analyze the fluorescence intensity of MPs pre-and postdigestion. MPs (n = 3) were dissolved in THF in a ratio such that the optical density (OD) of the solution was 1 ± 0.01 at an absorbance wavelength 257 nm.…”

Section: Spectral Analysesmentioning

confidence: 99%

“…[ 28 ] Similarly, PL has been used to infer polymer degradation by measuring fluorescence quenching of the incorporated dye. [ 17 ] Another lesser used quantitative method, often employed by polymer scientists and engineers, is gel permeation chromatography (GPC), which analyses the average molecular weight distribution. This technique provides direct evidence of polymer chain degradation.…”

Section: Introductionmentioning

confidence: 99%

“…[11] For this reason, chemical digestions in combination with high temperatures and prolonged treatment times are commonly utilized to achieve more robust and efficient MP recovery. [11,[14][15][16][17][18][19][20][21][22] However, these digestion treatments often use alkali (e.g., alkali hydroxides including potassium hydroxide (KOH) and sodium hydroxide (NaOH)), oxidative (e.g., hydrogen peroxide (H 2 O 2 )) and/or acidic (e.g., nitric acid (HNO 3 )) reagents which can react with, deform and/or completely deteriorate the MP. [15,[18][19][20]23] The use of particularly harsh conditions, e.g., full immersion in 69% HNO 3 at high temperatures (>80 °C) and/or for prolonged exposure times (>1 month), to digest tissues such as mussels, whole juvenile fish as well as tissue and stomach samples from invertebrates has been reported.…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Effect of Chemical Digestion Treatments on Polystyrene Microplastics: Recommended Updates to Chemical Digestion Protocols

Gulizia

Brodie

Daumuller

et al. 2022

Macro Chemistry & Physics

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Establishing the toxicity and exposure consequences of microplastics (MPs) on marine organisms relies on the nondestructive isolation of plastics from biological matrices. MPs are commonly extracted from these matrices by chemical digestion using alkali (e.g., potassium hydroxide (KOH) and sodium hydroxide (NaOH)), oxidative (e.g., hydrogen peroxide (H 2 O 2 )) and/or acidic (e.g., nitric acid (HNO 3 )) reagents. Although these digestion conditions can be highly effective for MP extraction, they can also react with the plastics. This can attribute an inaccurate representation of plastic contamination by altering MP visual characteristics (size, shape, color), thereby impeding identification and potentially returning erroneous numbers of ingested particles. In this study, the degradative impacts are assessed of the routinely applied digestion reagents (i) KOH, (ii) NaOH, (iii) H 2 O 2 , and (iv) HNO 3 on polystyrene (PS) based MPs sized between 200 μm and 5 mm. Degradation of the PS MPs is evaluated using FT-IR, gel permeation chromatography, NMR, photoluminescence spectroscopy, and microscopy. These studies reveal HNO 3 to be the most destructive for PS MPs, while the alkali and oxidative reagents result in negligible changes in plastic properties. These results are recommended to be used as a guideline to update current protocols to ensure the nondestructive treatment of MPs.

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Section: Spectral Analysesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating the Effect of Chemical Digestion Treatments on Polystyrene Microplastics: Recommended Updates to Chemical Digestion Protocols

Gulizia

Brodie

Daumuller

et al. 2022

Macro Chemistry & Physics

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“…Our previous study has found that three fluorescent polystyrene MPs in biological samples could be extracted with KOH without changing the characterization of the MPs [9]. As shown in figure 2, there was a clear correlation between the concentrations of the MPs and fluorescence intensity, with R 2 of 0.9996, 0.9939 and 0.9951, respectively.…”

Section: Determination Of Microplastic Detection Methodsmentioning

confidence: 84%

Transfer of Polystyrene Microplastics with Different Functional Groups in the Aquatic Food Chain

Sun²,

Li³

2023

J. Phys.: Conf. Ser.

Self Cite

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Microplastic pollution has become an increasingly concerning environmental problem because of its adverse impact on aquatic ecosystem security. Microplastics in the aquatic environment are poisonous to aquatic life, and they can not only be consumed by aquatic life but also pass down the food chain, endangering human health in the process. To investigate the accumulation and transfer of three different functional microplastics, including polystyrene (PS), carboxylate-modified polystyrene (PS-COOH), and amine-modified polystyrene (PS-NH2), in the aquatic food chain between Chlorella pyrenoidosa,Daphnia magna, and zebrafish, this study focused on three different functional microplastics. The maximum concentration of the three microplastics in Daphnia magna occurred at 8h, while that in zebrafish appeared at 24h. Three microplastics accumulated in the following order: PS-COOH > PS-NH2 > PS. The BMF of zebrafish intestine/Daphnia magna > 1, suggesting that biomagnification occurred along the food chain transfer and mainly accumulated in the intestine of zebrafish. This study confirmed the biomagnification of three polystyrene microplastics during food chain transfer, providing a theoretical basis for the transfer of microplastics in aquatic food chains.

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“…PS-NP distribution in parental tissues and offspring was observed using tissue sections combined with fluorescence microscopy (Eclipse 80i, Nikon, Tokyo, Japan) and fluorescence microscope alone, respectively. The quantitative determination of PS-NPs was carried out by KOH digestion 42 and fluorescence spectrophotometry. The mean recoveries of PS-NPs ranged from 80% to 89%.…”

Section: Quantification Of Ehs and Ps-nps In F0 And F1 Eggsmentioning

confidence: 99%

Effects of polystyrene nanoplastics on the bioaccumulation, distribution and parental transfer of ethylhexyl salicylate

Zhou

Yan

et al. 2022

Environ. Sci.: Nano

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Comparison of six digestion methods on fluorescent intensity and morphology of the fluorescent polystyrene beads

Cited by 26 publications

References 42 publications

Evaluating the Effect of Chemical Digestion Treatments on Polystyrene Microplastics: Recommended Updates to Chemical Digestion Protocols

Evaluating the Effect of Chemical Digestion Treatments on Polystyrene Microplastics: Recommended Updates to Chemical Digestion Protocols

Transfer of Polystyrene Microplastics with Different Functional Groups in the Aquatic Food Chain

Effects of polystyrene nanoplastics on the bioaccumulation, distribution and parental transfer of ethylhexyl salicylate

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