Comparison of Six Density Gradient Media for Selection of Cryopreserved Donor Spermatozoa

Abstract

The aim of our study was to evaluate the efficiency of 4 density gradient media for motile cryopreserved spermatozoa selection to Percoll (Kabi Pharmacia, Uppsala, Sweden) and to Puresperm (J.C.D. International Laboratory, L'Aigle, France). Puresperm was the new medium chosen in our laboratory in 1996 as the substitute for Percoll. The solutions tested were 3 colloidal silane-coated silica particle media (Isolate, SpermGrad-100, Sil-Select Plus) and iodixanol (Optiprep). Semen parameters analyzed after selection were concentration, motility, and morphology. Semen parameters after Puresperm gradient had similar values compared to Percoll. Optiprep was less efficient with a poor concentration. Isolate had a comparatively better concentration, but the capacity of selection was not satisfactory. SpermGrad-100 and Sil-Select Plus were less effective than Puresperm. In conclusion, Puresperm could be considered a better alternative to Percoll for cryopreserved spermatozoa migration.

Salvaging urospermic ejaculates from brown bear (Ursus arctos)

“…In agreement with these results, the centrifugation of dog semen in PureSperm ® density-gradient improved the motility and viability of frozen-thawed spermatozoa (Dorado et al, 2011). It has also been documented that PureSperm ® could be considered to select human fresh and frozen-thawed spermatozoa used in assisted reproductive techniques (Mousset-Simeon et al, 2004). However, Centola et al (1998) observed that sperm separated by the PureSperm ® was not different in motility as compared with fresh specimens but had the greatest percentage of hyper-active sperm.…”

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

“…In order to select a better population of sperm for these techniques, semen is usually processed, either through a swim-up or a density gradient centrifugation technique, and these have both proven to be efficient methods for selecting motile and morphologically normal sperm [19]. Centrifugation through a density gradient also removes leukocytes, cellular debris, and dead or immotile sperm [20].…”

Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa

“…Density-gradient centrifugation is commonly used for sperm preparation, which leads to an increased conception rate (1). This technique separates superior motile spermatozoa with normal morphology from the total sperm population, leaving behind immature, morphologically abnormal, and senescent spermatozoa with damaged DNA (2)(3)(4)(5)(6).…”