Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa

Mahfouz, R.; Sharma, Rakesh K.; Said, Tamer M.; Ērenpreiss, Juris; Agarwal, Ashok

doi:10.1016/j.fertnstert.2008.01.047

Cited by 38 publications

(20 citation statements)

References 59 publications

(72 reference statements)

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“…Alternatively, it could be the result of free-radical induced damage (43) or a consequence of apoptosis (44). The observed findings of the present study that infertile men with poor semen quality have increased DNA fragmentation agree with many other studies that established the fact that infertile men have higher DNA strand damage than fertile men (particularly in smokers) (28,32,(44)(45)(46)(47). Moreover, % of DNA fragmentation was significantly negatively correlated with each of semen parameters.…”

Section: Discussionsupporting

confidence: 89%

Apoptotic markers in semen of infertile men: association with cigarette smoking

El-Melegy

Ali

2011

Int. braz j urol.

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Objectives: (i) To examine the role of apoptosis in the pathogenesis of DNA damage in semen from infertile men. (ii) To assess the effects of smoking on apoptotic markers and seminal parameters among infertile men. (iii) To assess the correlation of apoptosis with conventional semen parameters. Materials and Methods:The study was carried out on 70 men with idiopathic infertility, divided into two groups: thirty infertile non smokers and forty infertile smokers. In addition to 60 fertile men (30 non smokers and 30 smokers) as control group. Each subject provided semen for analysis of parameters, determination of % of DNA fragmentation, s-Fas, caspase-3 activity levels and cotinine levels. Results:The results revealed that infertile men, particularly smokers have significantly lower semen variables and significantly higher levels of apoptotic variables (% of DNA fragmentation, s-Fas and caspase-3 activity) in addition to cotinine. Conclusions:The present findings provide additional evidence supporting the importance of the evaluation of apoptotic markers to test male infertility particularly among smokers.

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Section: Discussionsupporting

confidence: 89%

Apoptotic markers in semen of infertile men: association with cigarette smoking

El-Melegy

Ali

2011

Int. braz j urol.

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“…But, irrespective of the insult, spermatozoa undergoing early apoptosis and unrecognized by currently used methodologies in the standard clinical laboratory may be dysfunctional, and as such, may be unable to achieve fertilization. But more dramatically, they may pose the risk of carrying damaged DNA into the egg resulting in poor embryo development, miscarriage, or other untoward negative effects (10,19,(50)(51)(52).…”

Section: Discussionmentioning

confidence: 99%

The effect of age on the expression of apoptosis biomarkers in human spermatozoa

Colin

Barroso

Gomez‐Lopez

et al. 2010

Fertility and Sterility

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“…Fuorescence microscopy of samples stained with acridine orange, aniline blue and toluidine blue, acridine orange-propidium iodide is used for identification of sperm nuclei with the abnormal chromatin condensation and the ploidy (Erenpreiss et al 2001, Erenpreisa et al 2002, Bungum et al 2007, Mahfouz et al 2009, Moskovtsev et al 2009, Lazaros et al 2011, Montjean et al 2012.…”

Section: Evaluation Of Sperm Morphologymentioning

confidence: 99%

“…The labeling of DNA strand breaks (the terminal transferased UTP nick-end labeling (TUNEL) assay), Annexin V-propidium iodide assay and Sperm Chromatin Structure Assay are used for the detection and quantification of apoptosis in sperm (DNA fragmentation) (Erenpreisa et al 2002, Ricci et al 2002, Oosterhuis and Vermes 2004, Mahfouz 2009, Rylander et al 2009, Gatimel et al 2013, Gomez-Lopez et al 2013, Ruvolo et al 2013.…”

Section: Evaluation Of Sperm Morphologymentioning

confidence: 99%

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

Lasienë

Gedrimas

Vitkus

et al. 2013

Polish Journal of Veterinary Sciences

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The quality of sperm has a direct influence on the fertilization and developmental competence of embryos. In the literature we did not find defined criteria for evaluation of normal sperm parameters in various species of domestic mammals. Therefore we attempted to review evaluation of criteria of morphologically normal human sperm and their abnormalities. All sperm cells observed in the stained sample are classified as normal or abnormal. Any abnormalities in morphology of sperm have a negative effect on the outcome in in vitro fertilization and intracytoplasmic sperm injection. Abnormal sperm are categorized into subgroups according to the observed defects (concerning the head and/or midpiece and/or tail). Most morphologically abnormal sperm have multiple defects. This article can be considered as guideline for the manual of sperm quality evaluation in different species of domestic mammals.

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Association of sperm apoptosis and DNA ploidy with sperm chromatin quality in human spermatozoa

Cited by 38 publications

References 59 publications

Apoptotic markers in semen of infertile men: association with cigarette smoking

Apoptotic markers in semen of infertile men: association with cigarette smoking

The effect of age on the expression of apoptosis biomarkers in human spermatozoa

Evaluation of morphological criteria of sperm quality before in vitro fertilization and intracytoplasmic sperm injection

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