Comparison of single nucleotide polymorphisms in the human interleukin-10 gene promoter between rheumatoid arthritis patients and normal subjects in Malaysia

Abstract

In this study, three single nucleotide polymorphisms (SNPs) located within the promoter of the human interleukin (IL)-10 gene [rs1800896 (position: -1087G>A), rs1800871 (position: -824C>T) and rs1800872 (position: -597C>A)] were investigated in 84 rheumatoid arthritis (RA) patients and 95 age- and sex-matched healthy subjects using polymerase chain reaction-restriction fragment length polymorphism method. Production of IL-10 by peripheral blood lymphocytes from the RA patients and healthy subjects cultured in the presence of Concanavalin A (Con A) was determined by using enzyme-linked immunosorbent assay. The results show that the distribution of the IL-10 genotypes did not differ significantly between RA patients and healthy subjects (P>0.05). However, a significant difference was observed in allele frequencies of -824CT, -824TT, -597CA, and -597AA between the RA patients and healthy volunteers (P=0.04). The -1087A/-824T/-597A (ATA) haplotype, which comprises all mutant alleles, was associated with lower IL-10 production when compared with the other haplotypes. In contrast, the RA patients who did not display the ATA haplotype produced significantly higher levels of IL-10 when compared with those carrying either one (P=0.012) or two (P=0.005) ATA haplotypes. Our findings suggest that there is an association between SNPs in the promoter of the human IL-10 gene and susceptibility to RA.

Interleukins and interleukin receptors in rheumatoid arthritis: Research, diagnostics and clinical implications

“…In a Malaysian population the distribution of the IL-10 genotypes did not differ significantly between RA patients and healthy controls. However, significant difference was found in the allele frequencies of rs1800896CT, rs1800871TT, rs1800872CA and AA between the RA patients and healthy subjects [80] . A Polish population study could not show association between IL-10 genotypes and age at disease diagnosis, disease activity in a physician's global assessment, joint and extra-articular involvement.…”

Single Nucleotide Polymorphism in the Promoter of the Human Interleukin-13 Gene Is Associated with Asthma in Malaysian Adults

“…Detection of IL-13 production by Concanavalin A- (Con A-) stimulated peripheral blood leucocytes (PBL) from the asthma patients and healthy volunteers was performed as described previously [24]. Briefly, 4 mL of blood from asthma patients and healthy volunteers was collected into heparin tube (Meus, Piove di Sacco, Italy).…”

Association entre le polymorphisme de l’interleukine 10 et les marqueurs biologiques de l’inflammation chez des patients de l’ethnie Han de l’Est de la Chine atteints de polyarthrite rhumatoïde

“…Dans une étude effectuée dans une population hollandaise de PR, le génotype de l'IL-10 n'était pas associé à l'incidence de la PR, mais le polymorphisme du promoteur -2849 de l'IL-10 était associé avec la production d'auto-anticorps et avec la survenue ultérieure des lésions articulaires [16]. Les études ayant porté sur des populations asiatiques suggèrent une association entre le polymorphisme de l'IL-10 rs1800872 A/C et la PR [17][18][19].…”