Comparison of silver nanoparticle-induced inflammatory responses between healthy and metabolic syndrome mouse models

Kobos, L; Alqahtani, Saeed; Xia, Li; Coltellino, Vincent; Kishman, Riley; McIlrath, Daniel R.; Pérez-Torres, Carlos J.; Shannahan, Jonathan H.

doi:10.1080/15287394.2020.1748779

Cited by 21 publications

(15 citation statements)

References 94 publications

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“…This is a well-established model for producing mice with metabolic syndrome (MetS) [27,28]. Our previous work has generated consistent results with very little variability in inducing MetS by using these diets [19][20][21]. Specifically, we have established these diets result in approximately a 50% weight gain and over a 30% increase in total cholesterol levels and 6% in insulin without altering hemoglobin A1C [19,21].…”

Section: Animal Modelmentioning

confidence: 69%

“…MetS is associated with the development of insulin resistance and underlying inflammation. Our previous studies have not demonstrated differential levels of circulating insulin or inflammatory markers within the lung at this stage in MetS disease development compared to healthy mice [20,21]. Disease-associated alterations in these proteins may occur prior changes in clinical markers of insulin resistance and inflammation.…”

Section: Discussionmentioning

confidence: 80%

“…Our previous work has generated consistent results with very little variability in inducing MetS by using these diets [19][20][21]. Specifically, we have established these diets result in approximately a 50% weight gain and over a 30% increase in total cholesterol levels and 6% in insulin without altering hemoglobin A1C [19,21]. Mice were housed in a room with constant temperature, 12/12-h light/dark cycle.…”

Section: Animal Modelmentioning

confidence: 79%

“…Original NPs and those with the BCs were diluted to a concentration of 25 µg/mL and characterized via assessment of hydrodynamic size, polydispersity index, and ζ-potential (ZetaSizer Nano, Malvern Panalytical Ltd., Malvern, UK) (n = 3/particle). These methods have been previously utilized by our laboratory and other to characterize and verify NPs and NP-BCs [19][20][21][22]29,30].…”

Section: Np-bc Formation and Characterizationmentioning

confidence: 99%

“…Numerous diseases alter the biological milieu which may influence BC formation and contribute to susceptibility differences. Our previous research demonstrated unique BCs form in obese human serum compared to healthy resulting in altered cellular interactions and immune responses [19][20][21][22]. MetS modifies the biomolecule content within the circulation and also extend to changes in the lung.…”

Section: Introductionmentioning

confidence: 99%

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Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in Iron Oxide Nanoparticle-Biocorona Composition

et al. 2022

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Nanoparticles (NPs) interact with biomolecules by forming a biocorona (BC) on their surface after introduction into the body and alter cell interactions and toxicity. Metabolic syndrome (MetS) is a prevalent condition and enhances susceptibility to inhaled exposures. We hypothesize that distinct NP-biomolecule interactions occur in the lungs due to MetS resulting in the formation of unique NP-BCs contributing to enhanced toxicity. Bronchoalveolar lavage fluid (BALF) was collected from healthy and MetS mouse models and used to evaluate variations in the BC formation on 20 nm iron oxide (Fe3O4) NPs. Fe3O4 NPs without or with BCs were characterized for hydrodynamic size and zeta potential. Unique and differentially associated proteins and lipids with the Fe3O4 NPs were identified through proteomic and lipidomic analyses to evaluate BC alterations based on disease state. A mouse macrophage cell line was utilized to examine alterations in cell interactions and toxicity due to BCs. Exposures to 6.25, 12.5, 25, and 50 μg/mL of Fe3O4 NPs with BCs for 1 h or 24 h did not demonstrate overt cytotoxicity. Macrophages increasingly associated Fe3O4 NPs following addition of the MetS BC compared to the healthy BC. Macrophages exposed to Fe3O4 NPs with a MetS-BC for 1 h or 24 h at a concentration of 25 μg/mL demonstrated enhanced gene expression of inflammatory markers: CCL2, IL-6, and TNF-α compared to Fe3O4 NPs with a healthy BC. Western blot analysis revealed activation of STAT3, NF-κB, and ERK pathways due to the MetS-BC. Specifically, the Jak/Stat pathway was the most upregulated inflammatory pathway following exposure to NPs with a MetS BC. Overall, our study suggests the formation of distinct BCs due to NP exposure in MetS, which may contribute to exacerbated inflammatory effects and susceptibility.

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Section: Animal Modelmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 80%

Section: Animal Modelmentioning

confidence: 79%

Section: Np-bc Formation and Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in Iron Oxide Nanoparticle-Biocorona Composition

et al. 2022

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Long-term application of silver nanoparticles in dental restoration materials: potential toxic injury to the CNS

Wang,

Yin

et al. 2023

J Mater Sci: Mater Med

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Silver nanoparticles (AgNPs) have durable and remarkable antimicrobial effects on pathogenic microorganisms, such as bacteria and fungi, in dental plaques. As such, they are widely added to dental restoration materials, including composite resins, denture bases, adhesives, and implants, to solve the problems of denture stomatitis, peri-implant inflammation, and oral infection caused by the long-term use of these dental restoration materials. However, AgNPs can be absorbed into the blood circulatory system through the nasal/oral mucosa, lungs, gastrointestinal tract, skin, and other pathways and then distributed into the lungs, kidneys, liver, spleen, and testes, thereby causing toxic injury to these tissues and organs. It can even be transported across the blood-brain barrier (BBB) and continuously accumulate in brain tissues, causing injury and dysfunction of neurons and glial cells; consequently, neurotoxicity occurs. Other nanomaterials with antibacterial or remineralization properties are added to dental restoration materials with AgNPs. However, studies have yet to reveal the neurotoxicity caused by dental restoration materials containing AgNPs. In this review, we summarize the application of AgNPs in dental restoration materials, the mechanism of AgNPs in cytotoxicity and toxic injury to the BBB, and the related research on the accumulation of AgNPs to cause changes of neurotoxicity. We also discuss the mechanisms of neurotoxicity caused by AgNPs and the mode and rate of AgNPs released from dental restorative materials added with AgNPs to evaluate the probability of neurotoxic injury to the central nervous system (CNS), and then provide a theoretical basis for developing new composite dental restoration materials. Graphical Abstract

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A narrative review: CXC chemokines influence immune surveillance in obesity and obesity-related diseases: Type 2 diabetes and nonalcoholic fatty liver disease

Ullah

Din

Ding

et al. 2023

Rev Endocr Metab Disord

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Comparison of silver nanoparticle-induced inflammatory responses between healthy and metabolic syndrome mouse models

Cited by 21 publications

References 94 publications

Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in Iron Oxide Nanoparticle-Biocorona Composition

Modulation of Pulmonary Toxicity in Metabolic Syndrome Due to Variations in Iron Oxide Nanoparticle-Biocorona Composition

Long-term application of silver nanoparticles in dental restoration materials: potential toxic injury to the CNS

A narrative review: CXC chemokines influence immune surveillance in obesity and obesity-related diseases: Type 2 diabetes and nonalcoholic fatty liver disease

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