Comparison of Seven Commercial Tests for the Detection of Parvovirus B19-Specific IgM

Schwarz, Tino F.; Jäger, Gundula; Gilch, Sabine

doi:10.1016/s0934-8840(97)80114-4

Cited by 28 publications

(14 citation statements)

References 20 publications

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“…Thus, the cause of fetal disease would have been overlooked if diagnosis had been solely based on IgM testing. The lack of anti-B19 IgM reactivity can not be attributed to application of an insensitive assay since the assay we used was based on baculovirus expressed B19 antigen, which has been shown to yield better results than E. coli-expressed proteins or synthetic peptides (Bruu and Nordbø, 1995;Cohen and Bates, 1995;Sloots and Devine, 1996;Tolfvenstam et al, 1996;Schwarz et al, 1997). Furthermore, negative IgM results do not appear to be a consequence of an early developmental stage since only a small difference in gestational age was apparent between fetuses with and without IgM response: samples from IgM-positive fetuses were collected between 20 and 29 weeks (mean 24.4 weeks), and out of the nine IgM-negative fetuses, only three were at a gestational age of less than 20 weeks (18, 19, and 19 weeks, respectively).…”

Section: Discussionmentioning

confidence: 99%

Prenatal diagnosis of congenital parvovirus B19 infection: value of serological and PCR techniques in maternal and fetal serum

et al. 1999

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Intrauterine infection with parvovirus B19 (B19) is associated with non‐immune hydrops fetalis, miscarriage and stillbirth. Accurate laboratory tests for diagnosis of B19 infection are required to exclude other diagnoses. We analysed the diagnostic value of B19 IgM antibody testing and polymerase chain reaction (PCR) in the sera from 57 patients and their fetuses with abnormal ultrasonography. Viral DNA was found in 16 of the 58 fetuses (one twin pregnancy) whereas only 7 of these were tested positive for B19 IgM antibodies. The sera of all 16 mothers were also positive for B19 DNA. False‐positive B19 IgM results were obtained from two fetuses. The study highlights the limitations of B19 IgM serology and shows for the first time that, if a sensitive PCR assay is used, DNA measurement is the best indicator of infection not only in the fetal blood but also in the maternal blood. This improves the diagnostic value of the laboratory results considerably. DNA assays are essential in cases of doubtful serological results. Copyright © 1999 John Wiley & Sons, Ltd.

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Section: Discussionmentioning

confidence: 99%

Prenatal diagnosis of congenital parvovirus B19 infection: value of serological and PCR techniques in maternal and fetal serum

et al. 1999

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“…IgM capture assays will reliably detect a current or recent infection in immunocompetent persons (48,52,151,295). Accordingly, more than 85% of patients with erythema infectiosum or aplastic crisis due to B19 exhibit specific IgM (6), and these antibodies will remain detectable for 2 to 3 months following infection.…”

Section: Detection Of Antibodiesmentioning

confidence: 99%

Human Parvovirus B19

2002

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Parvovirus B19 (B19) was discovered in 1974 and is the only member of the family Parvoviridae known to be pathogenic in humans. Despite the inability to propagate the virus in cell cultures, much has been learned about the pathophysiology of this virus, including the identification of the cellular receptor (P antigen), and the control of the virus by the immune system. B19 is widespread, and manifestations of infection vary with the immunologic and hematologic status of the host. In healthy immunocompetent individuals B19 is the cause of erythema infectiosum and, particularly in adults, acute symmetric polyarthropathy. Due to the tropism of B19 to erythroid progenitor cells, infection in individuals with an underlying hemolytic disorder causes transient aplastic crisis. In the immunocompromised host persistent B19 infection is manifested as pure red cell aplasia and chronic anemia. Likewise, the immature immune response of the fetus may render it susceptible to infection, leading to fetal death in utero, hydrops fetalis, or development of congenital anemia. B19 has also been suggested as the causative agent in a variety of clinical syndromes, but given the common nature, causality is often difficult to infer. Diagnosis is primarily based on detection of specific antibodies by enzyme-linked immunosorbent assay or detection of viral DNA by dot blot hybridization or PCR. Treatment of persistent infection with immunoglobulin reduces the viral load and results in a marked resolution of anemia. Vaccine phase I trials show promising results

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“…Circulating IgM suggests the presence of an active infection, can be detected approximately 10 days after infection, and may remain positive for up to 6 months. IgG antibodies, which suggest a previous infection, are formed approximately 3 weeks after infection and may last for several years or even for life 24,25 . Maternal testing for parvovirus is not routinely performed for all pregnant women.…”

Section: Screening and Diagnosismentioning

confidence: 99%

Parvovirus B‐19 Infection During Pregnancy

Al-Khan

Caligiuri

Apuzzio

2003

Infectious Diseases in Obstetrics and Gynecology

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The development of an acute parvovirus B-19 infection during pregnancy can cause pregnancy complications ranging from early pregnancy loss to nonimmune hydrops. There is no treatment, but preventive measures can be used to decrease perinatal mortality. The diagnosis is made on the basis of clinical suspicion and serology. If the fetus exhibits hydrops in the latter part of pregnancy, the main treatment options include either correcting the associated anemia with intrauterine blood transfusion or birth with extrauterine management. Although the serious problems associated with this virus during pregnancy are uncommon, they can be fatal. In view of this, a pregnant woman who is antibody negative should try to avoid contact with large groups of young children in order to decrease contact with potential vectors.

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Comparison of Seven Commercial Tests for the Detection of Parvovirus B19-Specific IgM

Cited by 28 publications

References 20 publications

Prenatal diagnosis of congenital parvovirus B19 infection: value of serological and PCR techniques in maternal and fetal serum

Prenatal diagnosis of congenital parvovirus B19 infection: value of serological and PCR techniques in maternal and fetal serum

Human Parvovirus B19

Parvovirus B‐19 Infection During Pregnancy

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