Comparison of Selenium Source in Preventing Oxidative Stress in Bovine Mammary Epithelial Cells

Abstract

Oxidative stress can cause cell damage. Hydroxy-selenomethionine (HMSeBA) is an organic Se source with emerging antioxidant advantages. The objective of this study was to compare the effects of HMSeBA, selenomethionine (SeMet) and sodium selenite (SS) on the antioxidant response and the ability to resist oxidative stress in bovine mammary epithelial cells (BMEC). The BMEC were treated with 0 (Control), 20, 50, 100 and 150 nM HMSeBA, 100 nM SeMet and100 nM SS for 48 h. The results showed that HMSeBA and SeMet treatments had higher glutathione peroxidase (p < 0.01) and catalase (p = 0.01) activities and mRNA abundance of GPX3 (p = 0.02), but lower superoxide dismutase activity compared with SS (p = 0.04). The catalase activity (p < 0.05) and mRNA abundance of GPX3 (p = 0.04) changed in a quadratic manner with the increase of HMSeBA levels. To assess the potential protection of different Se sources against oxidative stress on BMEC, 0 or 50 μM H2O2 was added to BMEC culture for 3 h after Se pre-treatment for 48 h. The results showed that HMSeBA and SeMet, which did not differ (p > 0.05), but further decreased malondialdehyde and reactive oxygen species production compared with SS (p < 0.05). In conclusion, HMSeBA showed an enhanced cellular antioxidant status to resist oxidative damage induced by H2O2 when compared with SS, whereas the effects were similar to SeMet.

Effects of organic and inorganic selenium on selenium bioavailability, growth performance, antioxidant status and meat quality of a local beef cattle in China

“…In contrast, Cozzi et al reported that organic Se has better meat quality than inorganic Se ( 31 ). Recently, Sun et al reported that SM and hydroxy-selenomethionine (HMSeBA) increased the activity and mRNA abundance of GSH-Px and decreased the activities of SOD and ROS in bovine mammary epithelial cells compared with SS ( 40 ). Gong et al suggested that dietary organic Se supplementation was more efficient in increasing GSH-PX activity and total antioxidant capacity, as well as decreasing the MDA content compared to inorganic Se supplementation ( 41 ).…”

RNA-Seq Reveals the Role of miR-29c in Regulating Inflammation and Oxidative Stress of Bovine Mammary Epithelial Cells

“…When endogenous or exogenous ROS and stimulation factors, such as LPS, stimulate bMECs, the oxidative stress and inflammatory response generated by bMECs reduce vitality and affect the health of the mammary gland, resulting in lactation dysfunction, which is the main cause of cow mastitis (27)(28)(29)(30). The occurrence of mastitis leads to extensive changes in transcriptome profiles, including miRNA.…”

Hydroxy-Selenomethionine, an Organic Selenium Source, Increases Selenoprotein Expression and Positively Modulates the Inflammatory Response of LPS-Stimulated Macrophages

“…Previous studies have demonstrated that organic Se sources confer a better protective effect than inorganic sources against different oxidative stress conditions [ 32 , 38 , 39 , 40 , 41 ]. Therefore, upregulation of GPX1 and SELENOP gene expression by OH-SeMet and a further decrease in ROS production induced by H 2 O 2 could maintain effective antioxidant defenses and would help the cell to adapt to and overcome the stress with minimal negative consequences.…”