Comparison of sample preparation methods combined with fast gas chromatography – mass spectrometry for ultratrace analysis of pesticide residues in baby food

Abstract

Original PaperComparison of sample preparation methods combined with fast gas chromatography -mass spectrometry for ultratrace analysis of pesticide residues in baby food Four sample preparation techniques were compared for the ultratrace analysis of pesticide residues in baby food: (a) modified Schenck's method based on ACN extraction with SPE cleaning; (b) quick, easy, cheap, effective, rugged, and safe (QuEChERS) method based on ACN extraction and dispersive SPE; (c) modified QuEChERS method which utilizes column-based SPE instead of dispersive SPE; and (d) matrix solid phase dispersion (MSPD). The methods were combined with fast gas chromatographic-mass spectrometric analysis. The effectiveness of clean-up of the final extract was determined by comparison of the chromatograms obtained. Time consumption, laboriousness, demands on glassware and working place, and consumption of chemicals, especially solvents, increase in the following order QuEChERS a modified QuEChERS a MSPD a modified Schenck's method. All methods offer satisfactory analytical characteristics at the concentration levels of 5, 10, and 100 lg/kg in terms of recoveries and repeatability. Recoveries obtained for the modified QuEChERS method were lower than for the original QuEChERS. In general the best LOQs were obtained for the modified Schenck's method. Modified QuEChERS method provides 21 -72% better LOQs than the original method.

MSPD as sample preparation method for determination of selected pesticide residues in apples

“…The original QuEChERS method (Anastassiades, 2003) does not involve the solvent exchange step and therefore it is the fastest and least laborious method. In terms of time, space, and financial demands, laboriousness, material, and solvent consumption, the following order was established: QuEChERS, modified QuEChERS, MSPD and the Schenck's method (Hercegová, 2006). The increased urgency to reduce solvent consumption and manual labour led to commercial introduction of several alternative extraction approaches.…”

“…The most common raw material for baby food production in Slovakia is apple. The matrix of apple is complex; therefore, the sample preparation represents one of the most critical parts of the analysis Dömötorová, 2005;Hercegová, 2006). In general, sample preparation methods for nonfatty foods include liquid extractions followed by a clean-up method, e.g.…”

Pesticide Residue Analysis in Animal Origin Food: Procedure Proposal and Evaluation for Lipophilic Pesticides

“…[12][13], solid phase micro-extraction [14], matrix solid phase dispersion [15] and supercritical fluid extraction [16]; (ii) clean-up by normal phase liquid chromatography (NPLC) [17] and/or gel permeation chromatography (GPC) [18][19], solid phase extraction (SPE) [20][21], and dispersive solid phase extraction (d-SPE) [22]; (iii) concentration according to analytical technique. In recent years, QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) methodology (used for extraction and clean-up steps) has been applied with success on several non-fatty (< 2 %) food matrices such fruits and vegetables [23][24] and low-fatty (2-20 %) food matrices, such milk, egg and avocado [25].…”

Desenvolvimento e validação do método QuEChERS na determinação de resíduos de medicamentos veterinários em leite e carne de búfalo

“…Este método tem sido adaptado para ser aplicado a matrizes alimentícias mais complexas, que contêm alto conteúdo de lipídios e proteínas, como ovo e abacate, 14 chocolate, café e cereal, 15 alimento para bebês, 16 sementes de linhaça e amendoim, 17 azeitona e azeite de oliva, 18 tecido animal 19,20 e leite. [21][22][23] A cromatografia representa o mais relevante conjunto de técnicas analíticas disponíveis atualmente na análise de substâncias químicas, em especial a cromatografia gasosa, devido aos baixos limites de detecção que podem ser conseguidos com a técnica.…”