Comparison of salting-out and sugaring-out liquid–liquid extraction methods for the partition of 10-hydroxy-2-decenoic acid in royal jelly and their co-extracted protein content

Tu, Xijuan; Sun, Fanyi; Wu, Siyuan; Liu, Weiyi; Gao, Zhaosheng; Huang, Shaokang; Chen, Wenbin

doi:10.1016/j.jchromb.2017.12.020

Cited by 36 publications

(24 citation statements)

References 33 publications

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“…As shown in Figure 2b, EYs of the phenolic compounds were all larger than 97% when the concentration of MgSO 4 was 19 g/L in ACN–H 2 O mixture (50:50, v / v ). This higher partition efficiency of MgSO 4 compared with NaCl was similar with reports on the SALLE of dicarbonyl [28] and fatty acid [29] compounds, which can be attributed to the larger phase ratio in MgSO 4 -based SALLE than that in NaCl-based SALLE. As shown in Figure 2, EYs of more-polar phenolic compounds with LogD < 0 (caffeic acid, p -coumaric acid, isoferulic acid, dimethoxycinnamic acid, and cinnamic acid) in NaCl-based SALLE are lower than those in MgSO 4 .…”

Section: Resultssupporting

confidence: 88%

“…Increasing the initial concentration of ACN in ACN–H 2 O mixtures can lead to the increase of phase ratio [29]. Consequently, the improvement of EYs for more-polar compounds were observed.…”

Section: Resultsmentioning

confidence: 99%

“…The obtained EYs for more-polar compounds in SULLE are higher than NaCl-based SALLE, and are comparable with MgSO 4 -based SALLE. This could be attributed to the high phase ratio under the condition of high ACN concentration in SULLE [29].…”

Section: Resultsmentioning

confidence: 99%

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Comparison of the Partition Efficiencies of Multiple Phenolic Compounds Contained in Propolis in Different Modes of Acetonitrile–Water-Based Homogenous Liquid–Liquid Extraction

Chen

et al. 2019

Molecules

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Homogeneous liquid–liquid extraction (HLLE) has attracted considerable interest in the sample preparation of multi-analyte analysis. In this study, HLLEs of multiple phenolic compounds in propolis, a polyphenol-enriched resinous substance collected by honeybees, were performed for improving the understanding of the differences in partition efficiencies in four acetonitrile–water-based HLLE methods, including salting-out assisted liquid–liquid extraction (SALLE), sugaring-out assisted liquid–liquid extraction (SULLE), hydrophobic-solvent assisted liquid–liquid extraction (HSLLE), and subzero-temperature assisted liquid–liquid extraction (STLLE). Phenolic compounds were separated in reversed-phase HPLC, and the partition efficiencies in different experimental conditions were evaluated. Results showed that less-polar phenolic compounds (kaempferol and caffeic acid phenethyl ester) were highly efficiently partitioned into the upper acetonitrile (ACN) phase in all four HLLE methods. For more-polar phenolic compounds (caffeic acid, p-coumaric acid, isoferulic acid, dimethoxycinnamic acid, and cinnamic acid), increasing the concentration of ACN in the ACN–H2O mixture could dramatically improve the partition efficiency. Moreover, results indicated that NaCl-based SALLE, HSLLE, and STLLE with ACN concentrations of 50:50 (ACN:H2O, v/v) could be used for the selective extraction of low-polarity phenolic compounds. MgSO4-based SALLE in the 50:50 ACN–H2O mixture (ACN:H2O, v/v) and the NaCl-based SALLE, SULLE, and STLLE with ACN concentrations of 70:30 (ACN:H2O, v/v) could be used as general extraction methods for multiple phenolic compounds.

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Section: Resultssupporting

confidence: 88%

Section: Resultsmentioning

confidence: 99%

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Comparison of the Partition Efficiencies of Multiple Phenolic Compounds Contained in Propolis in Different Modes of Acetonitrile–Water-Based Homogenous Liquid–Liquid Extraction

Chen

et al. 2019

Molecules

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“…Royalisin, a 5.5 kDa protein, was found to have potentanti bacterial activity against Gramnegative bacteria. Protein profile of RJ is determined by different techniques such as two dimensional electrophoresis, LC-MS, MALDI-TOFF MS. SDS-PAGE and native PAGE electrophoresis were also used to characterize RJ (Gua, Kouzuma&Yonekura, 2009;Ramadana& Al-Ghamdi 2012;Sano et al, 2004;Tu et al, 2018). The aim of this study is to search a simple technique to display the royal jelly protein profile.…”

Section: Resultsmentioning

confidence: 99%

Royal Jelly and Its SDS-PAGE Electrophoresis Profiles

Çakır¹,

Şirin²,

Kolaylı³

2019

Journal of Apitherapy and Nature

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Royal jelly (RJ) is an important functional food, is used for many purposes. New methods are needed for thepractical characterization of royal jelly. In this preliminary study, SDS-PAGE electrophoretic profiles of royal jelly proteins were in vestigated. Raw royal jelly samples were analyzed by classical SDS-PAGE electrophoresis using 5% and 10% gels. The major protein spot were obtained at the samples collected same regions. Compared the proteins with known marker proteins they were between 5,2 and 116 kDa. Classical SDS-PAGE electrophoresis alone is not sufficient to determine royal jelly proteins, but can be used to basic characterization of royal jelly. Further selectrophoretic techniques are needed for a better separation.

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“…Before analytical instrumental analysis, sample preparations are often needed to remove potential interferences, to increase the concentration of the analyte, and to introduce low‐cost operation procedures . LLE and SPE were used as sample preparation techniques before the analysis of various samples. Compared to conventional SPE and LLE, microextraction techniques, such as single drop microextraction , hollow fiber liquid phase microextraction (HF–LPME) and dispersive liquid‐liquid microextraction (DLLME) have the advantage of being versatile, simple, cost‐effective, and more environmentally friendly.…”

Section: Introductionmentioning

confidence: 99%

Extraction of antidepressant drugs in biological samples using alkanol‐based nano structured supramolecular solvent microextraction followed by gas chromatography with mass spectrometric analysis

Salamat

Yamini

Moradi

et al. 2019

J of Separation Science

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In the present study, a supramolecular solvent was formed from reverse micelle aggregates of octanol. The proposed supramolecular solvent was used for rapid extraction of some antidepressants drugs including amitriptyline, imipramine, desipramine, maprotiline, sertraline, and doxepin from biological samples. Alkanol-based supramolecular solvents have a unique array of physicochemical properties, making them a very attractive alternative to replace organic solvents in analytical extractions. The parameters affecting the extraction of target analytes (i.e., the volume of tetrahydrofuran and octanol as the major components comprising the supramolecular solvent, chain length of alkanols, sample solution pH, salt addition, and ultrasonic time) were investigated and optimized by factor by factor optimization method. Under the optimum conditions, preconcentration factors of 470, 490, 460, 385, 370, and 430 were obtained for amitriptyline, doxepin, imipramine, desipramine, maprotiline, and sertraline, respectively. The linear ranges and coefficients of determination (R 2 ) were obtained in the range of 0.01-100 μg/L and 0.9974-0.9991, respectively. Also the limits of detection (S/N = 3) of 0.003-0.03 μg/L, and precisions (n = 5) of 4.9-8.9% were calculated. Finally, the method was successfully applied for the extraction of antidepressant drugs in biological samples, and relative recoveries in the range of 91-102% were obtained.

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Comparison of salting-out and sugaring-out liquid–liquid extraction methods for the partition of 10-hydroxy-2-decenoic acid in royal jelly and their co-extracted protein content

Cited by 36 publications

References 33 publications

Comparison of the Partition Efficiencies of Multiple Phenolic Compounds Contained in Propolis in Different Modes of Acetonitrile–Water-Based Homogenous Liquid–Liquid Extraction

Comparison of the Partition Efficiencies of Multiple Phenolic Compounds Contained in Propolis in Different Modes of Acetonitrile–Water-Based Homogenous Liquid–Liquid Extraction

Royal Jelly and Its SDS-PAGE Electrophoresis Profiles

Extraction of antidepressant drugs in biological samples using alkanol‐based nano structured supramolecular solvent microextraction followed by gas chromatography with mass spectrometric analysis

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