Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting

Akiba, Masato; Uchida, Ikuo; Nishimori, Kei; Tanaka, Kiyoshi; Anzai, Toru; Kuwamoto, Yasushi; Wada, Ryuichi; Ohya, Tatsuo; Ito, Hiroya

doi:10.1016/s0378-1135(02)00422-4

Cited by 22 publications

(21 citation statements)

References 13 publications

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“…Clamped homogeneous electric field electrophoresis was performed for PFGE using a CHEF-DR III apparatus (Bio-Rad Laboratories Inc., Hercules, CA, USA). Genomic DNA from each strain was prepared according to the method of Akiba et al (17). The genomic DNA in the sliced plugs was digested at 37°C for 6 h using 40 U of XbaI (TaKaRa Bio Inc., Shiga, Japan).…”

Section: Methodsmentioning

confidence: 99%

Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan

Kusumoto

Hikoda²,

Fujii

et al. 2016

J Clin Microbiol

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h Enterotoxigenic Escherichia coli (ETEC) and Shiga toxin-producing E. coli (STEC) are important causes of diarrhea and edema disease in swine. The majority of swine-pathogenic E. coli strains belong to a limited range of O serogroups, including O8, O138, O139, O141, O147, O149, and O157, which are the most frequently reported strains worldwide. However, the circumstances of ETEC and STEC infections in Japan remain unknown; there have been few reports on the prevalence or characterization of swine-pathogenic E. coli. In the present study, we determined the O serogroups of 967 E. coli isolates collected between 1991 and 2014 from diseased swine in Japan, and we found that O139, O149, O116, and OSB9 (O serogroup of Shigella boydii type 9) were the predominant serogroups. We further analyzed these four O serogroups using pulsed-field gel electrophoresis (PFGE), multilocus sequence typing, and virulence factor profiling. Most of the O139 and O149 strains formed serogroup-specific PFGE clusters (clusters I and II, respectively), whereas the O116 and OSB9 strains were grouped together in the same cluster (cluster III). All of the cluster III strains belonged to a single sequence type (ST88) and carried genes encoding both enterotoxin and Shiga toxin. This PFGE cluster III/ST88 lineage exhibited a high level of multidrug resistance (to a median of 10 antimicrobials). Notably, these bacteria were resistant to fluoroquinolones. Thus, this lineage should be considered a significant risk to animal production due to the toxigenicity and antimicrobial resistance of these bacteria.

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Section: Methodsmentioning

confidence: 99%

Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan

Kusumoto

Hikoda²,

Fujii

et al. 2016

J Clin Microbiol

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“…Pulsed-field gel electrophoresis (PFGE) was performed by clamped homogeneous electric field electrophoresis using a CHEF-DR III apparatus (Bio-Rad Laboratories, Inc., Hercules, CA). The genomic DNA of each strain was prepared as described by Akiba et al (20). Genomic DNA in sliced plugs was digested at 37°C with 40 U of XbaI for 6 h or 30 U of SspI for 16 h (both enzymes were obtained from TaKaRa Bio, Inc.).…”

Section: Methodsmentioning

confidence: 99%

Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine

Kusumoto

Fukamizu

Ogura

et al. 2014

Appl Environ Microbiol

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g Insertion sequences (ISs) are the simplest transposable elements and are widely distributed in bacteria; however, they also play important roles in genome evolution. We recently identified a protein called IS excision enhancer (IEE) in enterohemorrhagic Escherichia coli (EHEC) O157. IEE promotes the excision of IS elements belonging to the IS3 family, such as IS629, as well as several other families. IEE-mediated IS excision generates various genomic deletions that lead to the diversification of the bacterial genome. IEE has been found in a broad range of bacterial species; however, among sequenced E. coli strains, IEE is primarily found in EHEC isolates. In this study, we investigated non-EHEC pathogenic E. coli strains isolated from domestic animals and found that IEE is distributed in specific lineages of enterotoxigenic E. coli (ETEC) strains of serotypes O139 or O149 isolated from swine. The iee gene is located within integrative elements that are similar to SpLE1 of EHEC O157. All iee-positive ETEC lineages also contained multiple copies of IS629, a preferred substrate of IEE, and their genomic locations varied significantly between strains, as observed in O157. These data suggest that IEE may have been transferred among EHEC and ETEC in swine via SpLE1 or SpLE1-like integrative elements. In addition, IS629 is actively moving in the ETEC O139 and O149 genomes and, as in EHEC O157, is promoting the diversification of these genomes in combination with IEE.

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“…Pulsed-field gel electrophoresis was performed by clamped homogeneous electric field electrophoresis using a CHEFF DR III apparatus (Bio-Rad Laboratories). Genomic DNA of each strain was prepared by the method described by Akiba et al 48 Genomic DNA in sliced plugs was digested with 30 U of SspI at 37 °C for 16 h. Electrophoresis was conducted in a 1% agarose gel in 0.5× Tris-borate-EDTA buffer at 14 °C at 6 V cm − 1 for 10 h with a pulse time of 4 s.…”

Section: Dna Manipulationsmentioning

confidence: 99%

Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions

et al. 2011

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Insertion sequences (ISs) are the simplest transposable elements and are widely distributed in bacteria. It has long been thought that IS excision rarely occurs in bacterial cells because most bacteria exhibit no end-joining activity to regenerate donor DNA after IS excision. Recently, however, we found that excision of IS629, an IS3 family member, occurs frequently in Escherichia coli O157. In this paper, we describe a protein IS-excision enhancer (IEE) that promotes IS629 excision from the O157 genome in an IS transposase-dependent manner. Various types of genomic deletions are also generated on IEE-mediated IS excision, and IEE promotes the excision of other IS3 family members and ISs from several other IS families. These data and the phylogeny of IEE homologues found in a broad range of bacteria suggest that IEE proteins have coevolved with IS elements and have pivotal roles in bacterial genome evolution by inducing IS removal and genomic deletion.

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Comparison of Salmonella enterica serovar Abortusequi isolates of equine origin by pulsed-field gel electrophoresis and fluorescent amplified-fragment length polymorphism fingerprinting

Cited by 22 publications

References 13 publications

Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan

Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan

Lineage-Specific Distribution of Insertion Sequence Excision Enhancer in Enterotoxigenic Escherichia coli Isolated from Swine

Insertion sequence-excision enhancer removes transposable elements from bacterial genomes and induces various genomic deletions

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