Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan

Kusumoto, Masahiro; Hikoda, Yuna; Fujii, Yuki; Murata, Misato; Miyoshi, Hirotsugu; Ogura, Yoshitoshi; Gotoh, Yasuhiro; Iwata, Tadahisa; Hayashi, Tetsuya; Akiba, Masato

doi:10.1128/jcm.03141-15

Cited by 44 publications

(45 citation statements)

References 36 publications

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“…In our study, we have identified eight E. coli strains that were hybrids and carried virulence genes STEC–ETEC, which are largely restricted to five serotypes (O76:H19, O43:H2, O87:H16, OR: H2, and O152:H8). Several other studies reported STEC–ETEC hybrid strains; however, unlike our study, the serotypes were O2:H27, O101:H−, O15:H16, O74:H28, O116:H28, O128:H8, O136:H12, O141:H8, O168:H8, OX182:H16, OX182:H− in Finland (23), O139, O149, O116, and OSB9 in Japan (24), O100:H− in United States (21), and O2:H2 in Burkina Faso (42). …”

Section: Discussioncontrasting

confidence: 85%

Occurrence of Hybrid Escherichia coli Strains Carrying Shiga Toxin and Heat-Stable Toxin in Livestock of Bangladesh

Johura

Parveen

Islam

et al. 2017

Front. Public Health

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Shiga toxin-producing Escherichia coli (STEC) and enterotoxigenic E. coli (ETEC) are important causes of diarrhea in humans and animals worldwide. Although ruminant animals are the main source of STEC, diarrhea due to this pathotype is very low in Bangladesh where ETEC remains the predominant group associated with childhood diarrhea. In the present study, E. coli strains (n = 35) isolated from Bangladesh livestock (goats, sheep, and cattle) and poultry (chicken and ducks) were analyzed for the presence of major virulence factors, such as Shiga toxins (STX-1 and STX-2), heat-labile toxin, and heat-stable toxins (STa and STb). Multiplex polymerase chain reaction results revealed 23 (66%) E. coli strains to be virulent possessing either sta (n = 5), stx (stx1, n = 8; stx2, n = 2), or both (n = 8) genes in varying combinations. Thirty-four percent (8/23) of strains from livestock were hybrid type that carried both stx (either stx1 or stx2) and ETEC-specific enterotoxin gene sta. Serotyping results revealed that the ETEC strains belonged to five serotypes, namely O36:H5, O174:H−, O152:H8, O109:H51, and O8:H21, while the STEC-producing strains belonged to serotypes O76:H19 (n = 3), O43:H2 (n = 2), O87:H16 (n = 2), OR:H2 (n = 1), O110:H16 (n = 1), and O152:H8 (n = 1). The STEC–ETEC hybrid strains belonged to serotypes O76:H19 (n = 3), O43:H2 (n = 2), O87:H16, OR:H2, and O152:H8. Forty percent (2/5) of the ETEC and 20% (2/10) of the STEC strains were multidrug resistant with the highest drug resistance (50%) being found in the hybrid strains. Molecular fingerprinting determined by pulsed-field gel electrophoresis and cluster analyses by dendrogram revealed that, genetically, STEC–ETEC hybrid strains were highly heterogeneous. Multidrug-resistant E. coli STEC–ETEC hybrid strains in domesticated animals pose a public health threat for humans in Bangladesh.

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Section: Discussioncontrasting

confidence: 85%

Occurrence of Hybrid Escherichia coli Strains Carrying Shiga Toxin and Heat-Stable Toxin in Livestock of Bangladesh

Johura

Parveen

Islam

et al. 2017

Front. Public Health

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“…It is frequently detected in piglets with ED (Fairbrother et al, 2012, Vila et al, 2016. Kusumoto et al (2016) have reported that O149 is associated with F4 while O139 is associated with F18. The same result was found in this study.…”

Section: Discussionmentioning

confidence: 99%

“…The incidence of PWD and ED is mainly caused by virulence factors produced by pathogenic E. coli. Therefore, it is important to detect virulence factors of E. coli to diagnose colibacillosis (Duan et al, 2012;Kusumoto et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Enterotoxins of pathogenic E. coli include heat-labile toxin (LT), heat-stable toxin (ST), enteroaggregative E. coli heat-stable enterotoxin 1 (EAST-1), and Shiga toxin type 2e (Stx2e) (Duan et al, 2012;Kusumoto et al, 2016). Among these enterotoxins, LT and ST are related to enterotoxigenic E. coli (ETEC) which is a well-known cause of PWD.…”

Section: Introductionmentioning

confidence: 99%

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Serogroups, Virulence Genes and Antimicrobial Resistance of F4+ and F18+ Escherichia coli Isolated from Weaned Piglets

Do¹,

Byun²,

Lee³

2019

PVJ

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Ninety-one F4 + pathogenic Escherichia (E.) coli and 181 F18 + pathogenic E. coli were isolated from piglets suffering enteric colibacillosis during 2007-2016. These strains were analyzed for O-serogroups, adhesin genes (eae, paa, AIDA-1), toxin genes (LT, STa, STb, Stx2e, EAST-1), and their susceptibility to 16 antimicrobials using disc diffusion method. We found that O149 and O139 were predominant serogroups in F4 + E. coli (36.3%) and F18 + E. coli (16.6%), respectively. AIDA-1 was the most predominant adhesin gene in F18 + E. coli (26.5%) while paa was the most predominant adhesin gene in F4 + E. coli (30.8%). LT (70.3%), STb (84.6%), and EAST-1 (73.6%) were detected with high frequency in F4 + E. coli. However, STa (43.6%) and Stx2e (49.2%) were the predominant toxin genes detected in F18 + E. coli. Both F4 + and F18 + E. coli showed high resistance to tetracycline (F4 + : 91.2%, F18 + : 90.6%), chloramphenicol (F4 + : 87.9%, F18 + : 92.3%), and streptomycin (F4 + : 89.0%, F18 + : 84.0%). F18 + E. coli showed higher resistance to colistin (9.4%) rather than F4 + E. coli (2.2%). In summary, we compared serogroups, virulence factors, and antimicrobial susceptibility of F4 + and F18 + E. coli from diarrheic weaned piglets. Results of this study could be used to design control measures for enteric colibacillosis in piggeries.To Cite This Article: Do KH, Byun JW and Lee WK, 2019. Serogroups, virulence genes and antimicrobial resistance of F4 + and F18 + Escherichia coli isolated from weaned piglets. Pak Vet J, 39(2): 266-270. http://dx.

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“…F4 and F18 fimbriae have been frequently detected in weaned piglets in several countries such as Korea, Japan, Europe and United States (Do, Byun, & Lee, ; Kusumoto et al, ; Luppi et al, ; Zhang et al, ). It is interesting and surprising to observe such a high prevalence of genes enconding for fimbriae F5, F41, F6 in this study.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and characterization of virulence genes in Escherichia coli isolated from piglets suffering post‐weaning diarrhoea in Shandong Province, China

Wang²,

Zhou³

et al. 2019

Veterinary Medicine & Sci

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The present study was performed to investigate the prevalence and characteristics of virulence genes in Escherichia coli (E. coli) isolated from piglets suffering post‐weaning diarrhoea (PWD) in Shandong Province, China. The standard bacteriological method was used to isolate and identify E. coli, and then multiplex polymerase chain reaction (mPCR) was performed to determine virulence genes in E. coli. Among the 300 isolates, 166 (55.3％) harboured at least one virulence gene. Among the 166 isolates, 155 (93.4％) contained toxin‐related genes. For enterotoxin genes, EAST1 (58/166, 34.9％) and LT‐I (45/166, 27.1％) were the most common, followed by STa (32/166, 19.3％) and STb (21/166, 12.7％); for pathogenicity island (PAI) genes, irp2 (49/166, 29.5％) was the most dominant, followed by eae (48/166, 28.9％); for Shiga toxigenic E. coli (STEC)‐associated toxin genes, Stx2e and hlyA genes were observed in 19 (19/166, 11.4％) and three strains (3/166, 1.8％) respectively. In addition, of the 166 isolates, 95 (95/166, 57.2％) contained adhesin genes, and AIDA‐I (33/166, 19.9％) was the most common, followed by paa (27/166, 16.3％), F5 (K99) (20/166, 12.0％), F18 (15/166, 9.0％) and F41 (12/166, 7.2％). In summary, these findings demonstrated the prevalence and characteristics of virulence factors in E. coli isolates from piglets with PWD in Shandong Province of China, and the data may be useful for establishing preventive measures for post‐weaning piglet diarrhoea.

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Emergence of a Multidrug-Resistant Shiga Toxin-Producing Enterotoxigenic Escherichia coli Lineage in Diseased Swine in Japan

Cited by 44 publications

References 36 publications

Occurrence of Hybrid Escherichia coli Strains Carrying Shiga Toxin and Heat-Stable Toxin in Livestock of Bangladesh

Occurrence of Hybrid Escherichia coli Strains Carrying Shiga Toxin and Heat-Stable Toxin in Livestock of Bangladesh

Serogroups, Virulence Genes and Antimicrobial Resistance of F4+ and F18+ Escherichia coli Isolated from Weaned Piglets

Prevalence and characterization of virulence genes in Escherichia coli isolated from piglets suffering post‐weaning diarrhoea in Shandong Province, China

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