Comparison of saline, iodine and koh wet mount preparations for occurrence of parasites in stool samples from patients attending ICDDR,B

Abstract

Abstract:The purpose of the present study was to compare results of saline, iodine, and KOH wet mount preparations executed on each of 1116 stool samples from patients attending ICDDR,B clinical pathology laboratory was compared. Ascaris lumbricoides was most prevalent helminth (3.67%) found in KOH wet mount preparation. The protozoan parasites (9.59%) were more prevalent than helmiths (8.24%) and Giardia sp (4.03%) shows the higher infection found by KOH wet mount preparation. Females are more infected by parasites than males found in observations by all the three methods (saline, iodine and KOH wet mount preparation). It was also observed that the young (1-10 years) were more infected than older age group (above 51). In seasonal variation, parasitic infestation was higher during May (27%) and the prevalence was lower in December (10%). KOH was found to be effective in destroying the faecal debris, faecal mass and other artifacts without affecting many of the parasitic ova, cysts and larvae in the stool smears. KOH for wet mount smear in examining the stool samples for detection of intestinal helminths may be routinely used which is simple, cost-effective and could be afforded in resource poor setting.

Prevalence of Intestinal Parasite Infections in Stray Dogs in Taif Region, KSA

“…Concerning single and mixed protozoa infection, Giardia was of high prevalence (16.2%), followed by Cryptosporidium Prevalence of Intestinal Parasite Infections in Stray Dogs in Taif Region, KSA (8.5%) and finally E. histolytica (1.5%) in fecal samples of dogs. Protozoa appeared to have less shrinkage and a more distinct internal structure with the saline concentration than other mount preparations (Zaman et al, 2018). The present work recorded that the prevalence of Giardia was (16.2%), which was confirmed by the iodine wet mount method, where internal structures of trophozoites, cysts were stained well by iodine, the method that made their recognition and identification easier in the specimens.…”

“…and T. vulpis in North America. The A. lumbricoides has a prevalence of (7.7%), where the present study suggested that dogs could act as reservoir hosts of A. lumbricoides and environmental contaminators that increase the risk of infection in humans (Zaman et al, 2018).…”

“…Microscopic observation of wet mounts remains the most widely used method for identifying ova and cyst of parasites from stool specimens (Myers and Koshi, 2011). Some problems and art facts made the diagnosis of parasites difficult in fecal specimens (Zaman et al, 2018). So, the present study was carried out to determine the prevalence, intensity and types of intestinal parasites in stray dogs in the Taif region in Saudi Arabia and the detection of parasites with the aid of different mount stain techniques.…”

Prevalence of intestinal parasitic infection in patients at Wasit Province, Iraq

“…The table (2) showed the higher rates of infection in male than in female with parasites like Entamoeba histolytica, Giardia lamblia, Enterobius vermicularis were recorded by some researchers (28) and (29). On other hands, researchers ( 20), (30), (31) were recorded higher infection rate with intestinal parasites in female than male, and many studies observed an increase in the prevalence of intestinal parasitic infection among the males (23) and others found an equality between both genders (21,22,23). The findings of our study disagreed with (24) in Riyadh/ Saudi Arabia.…”