Comparison of Rp-HPLC and Uv Spectrophotometric Methods for Estimation of Haloperidol in Pure and Pharmaceutical Formulation

Abstract

An accurate, precise, sensitive and reproducible High-performance liquid chromatographic (HPLC) and UV spectrophotometric methods were developed and validated for the quantitative determination of haloperidol (HPD) in bulk drug and pharmaceutical formulation. Different analytical performance parameters such as linearity, precision, accuracy, specificity, limit of detection (LOD) and limit of quantification (LOQ) were determined according to International Conference on Harmonization ICH Q2B guidelines. The RP-HPLC method was developed by the isocratic technique on a reversed-phase Thermo C18 (250 × 4.6 mm, 5µm) column with mobile phase consisting of Methanol: Acetonitrile (50:50v/v) at flow rate of 1.0 ml/min. The retention time for HPD was 2.238±0.3min. The UV spectrophotometric determinations were performed at 244 nm using 80% methanol as a solvent. The linearity range for HPD was 5-25 μg/ml for both HPLC and UV method. The linearity of the calibration curves for each analyte in the desired concentration range was good (r2 >0.999) by both the HPLC and UV methods. The method showed good reproducibility and recovery with percent relative standard deviation less than 2%. Moreover, the accuracy and precision obtained with HPLC co-related well with the UV method which implied that UV spectroscopy can be a cheap, reliable and less time consuming alternative for chromatographic analysis. The proposed methods are highly sensitive, precise and accurate and hence successfully applied for determining the assay and in vitro dissolution of a marketed formulation.
 Keywords: HPLC, UV Spectrophotometry, Haloperidol, Pharmaceutical formulation, Method validation, Quantitative analysis

An Electrochemical Sensor Fabricated by Sonochemical Approach for Determination of the Antipsychotic Drug Haloperidol

“…In recent years, various analytical procedures have been developed for much worldwide research on the determination of antipsychotic drugs, quantifying the amount of commercial formulation products in biological samples such as human blood and urine, detecting degradation products, and determining the amount of residues [10][11][12]. The haloperidol drug is metabolized to several metabolites, and enzymes involved in the biotransformation of haloperidol include cytochrome P450, carbonyl reductase, and uridine diphosphoglucose glucuronosyltransferase [13,14]. The greatest proportion of intrinsic hepatic clearance of haloperidol drug is glucuronidation.…”

“…It is important that haloperidol and its derivatives, which undergo these processes, are eliminated from the body together with their metabolites after treating the diseases [15][16][17]. Considering its therapeutic importance, analytical techniques such as gas chromatography, electrochemical analysis, gas chromatography-mass spectrometry, reversed phase high performance quid chromatography, UV/Vis spectrophotometry, high-performance liquid chromatography, capillary electrophoresis, and high performance liquid chromatography mass spectrometry, are available for the determination of haloperidol in blood, urine and pharmaceutical preparations samples [18][19][20][21][22][23][24][25][26]. Among these methods, electrochemical analysis techniques are the most promising due to their ability to provide better stability, precision, low cost, reliability, and monitoring of kinetics of load transfer mechanisms involved in the oxidation or reduction process of pharmaceutical compounds [27].…”

Analytical Method Development and Validation for Estimation of Emtricitabine in Tablet Dosage Form by Reverse Phase High Performance Liquid Chromatography

“…The purpose of this study was to develop sensitive, simple, precise, accurate method for Emtricitabine by Ultraviolet (UV) and Reverse Phase High Performance Liquid Chromatography (RP HPLC). For that we use the simple mobile phase which are less expensive and easily available than other solvents [6][7][8][9][10][11][12] .…”