Comparison of ROS formation and antioxidant enzymes in Cleome gynandra (C4) and Cleome spinosa (C3) under drought stress

Abstract

a b s t r a c tDifferences between antioxidant responses to drought in C 3 and C 4 plants are rather scanty. Even, we are not aware of any research on comparative ROS formation and antioxidant enzymes in C 3 and C 4 species differing in carboxylation pathway of same genus which would be useful to prevent other differences in plant metabolism. With this aim, relative shoot growth rate, relative water content and osmotic potential, hydrogen peroxide (H 2 O 2 ) content and NADPH oxidase (NOX) activity, antioxidant defence system (superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), glutathione reductase (GR) enzymes and their isoenzymes), CAT1 mRNA level, and lipid peroxidation in seedlings of Cleome spinosa (C 3 ) and Cleome gynandra (C 4 ) species of Cleome genus exposed to drought stress for 5 and 10 day (d) were comparatively investigated. Constitutive levels of antioxidant enzymes (except SOD) were consistently higher in C. spinosa than in C. gynandra under control conditions. CAT1 gene expression in C. spinosa was correlated with CAT activity but CAT1 gene expression in C. gynandra at 10 d did not show this correlation. Drought stress caused an increase in POX, CAT, APX and GR in both species. However, SOD activity was slightly decreased in C. gynandra while it was remained unchanged or increased on 5 and 10 d of stress in C. spinosa, respectively. Parallel to results of malon dialdehyde (MDA), H 2 O 2 content was also remarkably increased in C. spinosa as compared to C. gynandra under drought stress. These results suggest that in C. spinosa, antioxidant defence system was insufficient to suppress the increasing ROS production under stress condition. On the other hand, in C. gynandra, although its induction was lower as compared to C. spinosa, antioxidant system was able to cope with ROS formation under drought stress.

Responses of the antioxidant defense system to drought stress in the leaves of Fargesia denudata seedlings, the staple food of the giant panda

“…This causes oxidative damage to cellular components and structures and disrupts metabolism, which finally leads to cell death [4]. Usually, such oxida tive damage can be assessed by lipid peroxidation [5,6].…”

Fitotoxidez de mesotriona em milho inoculado com Azospirillum brasilense associado a adubação nitrogenada

“…Isso principalmente pela ação da POD nos cloroplastos (Huseynova, 2012) e na remoção de ERO's produzidas pela fotoinibição (Uzilday et al, 2012).…”

Alteration of photosynthetic pigments and antioxidant systems in tomato under drought with Tebuconazole and Hexaconazole applications

“…Drought stress is associated with increased oxidative damage to plants due to the increased production of ROS, such as H2O2 and O2-, which is further reduced to O2 and H2O by POD and CAT (Uzilday et al, 2012). Previous studies on the responses of antioxidant defence systems in plant to drought stress have concentrated on various commercial crops (Cakmak and Marschner, 1992;Bai et al, 2006).…”