Comparison of Reverse Transcription - PCR and Viral Culture for Detection of Respiratory Syncytial Virus in Young Children : Relation to Epidemiological and Clinical Findings

Zahran, Wafaa A; Makled, Amal F.; Salama, Ahmed A; El-Hendaw, Ghada R.; Bader, Hassan S.

doi:10.12816/0046226

Cited by 5 publications

(9 citation statements)

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“…22 However, both the sexes were equally infected with RSV in our study. Similar observations were made by Fall et al 21 and Koetz et al 23 Zahlan et al 18 and Liu et al 24 documented male dominance in their study.…”

Section: Discussionsupporting

confidence: 89%

“…nRT-PCR was shown to detect significantly more numbers of RSV than tissue culture in a previous study. 18 In India, studies by Nandhini et al 29 and Agrawal et al 12 reported that performance of real time RT-PCR in detecting RSV was greater than that of conventional RT-PCR, supporting the findings of our study.…”

Section: Discussionsupporting

confidence: 89%

“…Varying prevalence rates were documented in different parts of the world. [15][16][17][18] The difference in epidemiology of RSV in the world could be due to variations in climatic conditions, environmental factors and severity of its epidemics.…”

Section: Discussionmentioning

confidence: 99%

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Epidemiology of respiratory syncytial virus infections in Chennai, south India

Hindupur

Menon

Dhandapani

2019

Clinical Epidemiology and Global Health

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 89%

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Epidemiology of respiratory syncytial virus infections in Chennai, south India

Hindupur

Menon

Dhandapani

2019

Clinical Epidemiology and Global Health

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“…Re infection in RSV and hMPV happens with comparable strains in spite of normal infection stimulating high levels of antibody against conserved antigens [11]. The diagnosis of RSV and hMPV infections can be made by several techniques, including culture, nucleic acid amplification tests (NAAT), antigen detection and serology test, but detection of viral RNA by NAAT such as reverse transcriptase-PCR (RT-PCR) assay is the most sensitive method for diagnosis of RSV and hMPV infections [12].…”

Section: Introductionmentioning

confidence: 99%

Detection of Respiratory Syncytial Virus and Human Metapneumovirus in Children with Respiratory Tract Infection by Use Real Time Polymerase Chain Reaction

Hussein¹,

Aufi²,

Al-Mayah³

2018

DJM

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Background:Respiratory tract illness is a major cause of morbidity and mortality among children, elderly and immunocompromised patients worldwide. Objective: To determine the infection rate of respiratory syncytial virus and human metapneumovirus among children with respiratory tract infection in Baghdad city. Patients and Methods: A cross sectional study was based on the processing of nasopharyngeal swabs from 150 children with acute respiratory tract infections, (81) males and (69)females; aged under five years old, who was admitted to Al-Imamin Al-Kadhimin Medical City and Pediatrics Protection Hospital in Baghdad during the period from December 2017 till April 2018. Nasopharyngeal swabs were collected from each participant and stored as frozen at -70 °C to use for RNA extraction and real time-polymerase chain reaction. Results: Out of all these samples, 54 samples were positive for respiratory syncytial virus (36%) and human metapneumovirus (1.33%). The infection rate of respiratory syncytial virus is more common in males (57.41%) than females and in children ≤ one year (37.04%) also high frequency were noticed among patients live in an urban area (72.22%) (50%) respectively and winter. According to different clinical feature, fever, cough, and wheezing were more common. Conclusion:The infection rate of respiratory syncytial virus was more than human metapneumovirus in children with respiratory tract infection using real time-PCR technique and the clinical manifestations were more common during respiratory syncytial virus and human metapneumovirus infection are cough, fever, wheezing.

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“…Conventionally, evaluation of the performance of RSV diagnostics has relied on the use of imperfect reference tests 15 – 18 that may introduce bias to the accuracy estimates of index tests. Further, a call for alternative evaluation approaches is justified especially when interest lies in demonstrating the superiority of a novel tests over an existing reference standard 19 .…”

Section: Introductionmentioning

confidence: 99%

Accuracy of diagnostic tests for respiratory syncytial virus infection within a paediatric hospital population in Kilifi County, Kenya

et al. 2020

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Background: Respiratory syncytial virus (RSV)-induced lower respiratory tract disease is a prominent cause of hospitalisation among children aged <5 years in developing countries. Accurate and rapid diagnostic tests are central to informing effective patient management and surveillance efforts geared towards quantifying RSV disease burden. This study sought to estimate the sensitivity (Se), specificity (Sp) (along with the associated factors) and predictive values of a direct immunofluorescence test (IFAT), and two real-time reverse transcription polymerase chain reaction (rRT-PCR) assays for RSV infection within a paediatric hospital population: a multiplex rRT-PCR (MPX) and Fast-Track Diagnostics® (FTD) Respiratory Pathogens 33 (Resp-33) rRT-PCR. Methods: The study enlisted 1458 paediatrics aged ≤59 months admitted with acute respiratory illness at the Kilifi County Hospital between August 2011 and December 2013. A Bayesian latent class modelling framework was employed to infer the tests’ estimates based on the patients’ diagnostic data from the three tests. Results: The tests posted statistically similar Se estimates: IFAT (93.7%, [90.7; 95.0]), FTD (97.8%, [94.6; 99.4]) and MPX (97.5%, [94.2; 99.3]). As for Sp, FTD registered a lower estimate (97.4%, [96.2; 98.2]) than MPX (99.7%, [99.0; 100.0]) but similar to IFAT (99.0%, [98.2; 99.6]). The negative and positive predictive values were strong (>91%) and closely mimicked the pattern given by the Se and Sp values respectively. None of the examined covariates (age, sex and pneumonia status) significantly influenced the accuracy of the tests. Conclusions: The evaluation found little to choose between the three diagnostic tests. Nonetheless, with its relative affordability, the conventional IFAT continues to hold promise for use in patient care and surveillance activities for RSV infection within settings where children are hospitalised with severe acute respiratory illness.

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Comparison of Reverse Transcription - PCR and Viral Culture for Detection of Respiratory Syncytial Virus in Young Children : Relation to Epidemiological and Clinical Findings

Cited by 5 publications

References 0 publications

Epidemiology of respiratory syncytial virus infections in Chennai, south India

Epidemiology of respiratory syncytial virus infections in Chennai, south India

Detection of Respiratory Syncytial Virus and Human Metapneumovirus in Children with Respiratory Tract Infection by Use Real Time Polymerase Chain Reaction

Accuracy of diagnostic tests for respiratory syncytial virus infection within a paediatric hospital population in Kilifi County, Kenya

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