Comparison of remineralization by fluoride varnishes with and without casein phosphopeptide amorphous calcium phosphate in primary teeth

Salman, Nehal R.; El-Tekeya, Magda M.; Bakry, Niveen S.; Omar, Sanaa H.; Tantawi, Maha El

doi:10.1080/00016357.2018.1490967

Cited by 12 publications

(14 citation statements)

References 24 publications

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“…Previous studies, in contrast to ours, reported higher effect of 5% NaF with CPP‐ACP varnishes on de‐remineralization process compared to varnishes containing only 5% NaF (Salman, ElTekeya, Bakry, Omar, & El Tantawi, 2019; Tuloglu, Bayrak, Tunc, & Ozer, 2016). The greater effect observed could be explained due to the synergistic effect of fluoride, calcium and phosphate offering greater remineralization capacity than the fluoride varnishes only.…”

Section: Discussioncontrasting

confidence: 95%

Fluoride release and remineralizing potential of varnishes in early caries lesions in primary teeth

Soares‐Yoshikawa

Varanda

Iwamoto

et al. 2020

Microscopy Res & Technique

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This study evaluated the fluoride (F) release and remineralizing potential of varnishes containing sodium fluoride (5% NaF), 5% NaF with CPP‐ACP and 5% NaF with TCP in early caries lesions in primary teeth. To determine the F release at 1, 4, 6, 24, 72, and 168 hr, strips were covered with the varnishes and immersed in purified water (n = 7). The varnishes and purified water (negative control) were applied on enamel blocks with early caries lesions (n = 16). Enamel blocks were stored in artificial saliva and submitted to a pH‐cycling. The area of enamel hardness loss (ΔS) was analyzed by microhardness, lesion depth by polarized light microscopy (PLM) and the chemical analysis by Energy‐dispersive X‐ray spectroscopy. Data were submitted to Shapiro–Wilk, two‐way and one‐way ANOVA, Tukey and paired t‐tests (α = 5%). All varnishes released F, but 5% NaF with CPP‐ACP had the highest release at 4, 6, 24, and 72 hr (p < .05) followed by 5% NaF with TCP and 5% NaF. No significant difference in ΔS was observed among varnishes (5% NaF = 4,098.4 ± 1,407.9; 5% NaF with CPP‐ACP = 4,164.0 ± 1,019.3; 5% NaF with TCP = 4,183.2 ± 1,527.2; p = .999), but all of them differed from the negative control group (6,757.8 ± 2,274.7; p < .001). Lesion depth was lower in varnishes groups compared to negative control (% reduction: 5% NaF = 41.8%, 5% NaF with CPP‐ACP = 38.8%, and 5% NaF with TCP = 36.3%; p < .001). Similar Ca, P, and Ca/P ratio percentages among groups and F was not detected after the treatments. All fluoride varnishes showed potential to enhance remineralization of early caries lesions in primary teeth.

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Section: Discussioncontrasting

confidence: 95%

Fluoride release and remineralizing potential of varnishes in early caries lesions in primary teeth

Soares‐Yoshikawa

Varanda

Iwamoto

et al. 2020

Microscopy Res & Technique

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“…To induce dental caries, the specimens were immersed in a BHI medium containing 2% sucrose and S. mutans , and polarized microscopy was used to measure the demineralization area 21 . Polarized light microscopy has been widely used to evaluate and measure the demineralization area of teeth specimens, especially for the evaluation of demineralization inhibition or remineralization effects of various materials or agents 38–40 . The extensive demineralization was shown in the negative control, which did not receive a LCFV application, indicated that the medium could effectively induce dental caries in the enamel surface (Fig.…”

Section: Discussionmentioning

confidence: 99%

Incorporation of zwitterionic materials into light-curable fluoride varnish for biofilm inhibition and caries prevention

Kim

Lee

Kim

et al. 2019

Sci Rep

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We incorporated zwitterionic materials into light-curable fluoride varnish (LCFV) in order to inhibit biofilm accumulation and prevent dental caries, and the properties of LCFV with three different zwitterionic materials, namely, 2-methacryloyloxyethyl phosphorylcholine (MPC), carboxybetaine methacrylate (CBMA), and sulfobetaine methacrylate (SBMA) polymers (each at a weight percentage of 3%), were compared; unmodified LCFV without any zwitterionic material was used as a control. Material properties including film thickness and degree of conversion (DC) of each type of LCFV were evaluated. In addition, protein-repellent effects and inhibitory effects on Streptococcus mutans adhesion and saliva-derived biofilm accumulation of LCFV were estimated. Finally, the preventive effect of LCFV on enamel demineralization was assessed in vitro on extracted human teeth specimens stored in S. mutans-containing medium. The film thickness of LCFV significantly decreased with the incorporation of zwitterionic materials compared to the control LCFV, whereas there were no significant differences in the DC among all of the LCFV groups. Furthermore, the amount of adsorbed protein, adherent S. mutans colony-forming unit (CFU) counts, and saliva-derived biofilm thickness and biomass were all significantly lower for LCFV with incorporated zwitterionic materials compared with the control. All LCFV groups including the control showed certain preventive effects against enamel demineralization during a 14-day immersion in the medium with S. mutans and sucrose, and the depth of demineralization was significantly lower in LCFV with zwitterionic materials than in the control. Thus, the incorporation of zwitterionic materials such as MPC, CBMA, and SBMA appears to confer superior antifouling effects to LCFV.

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“…Additionally, conventional techniques have been used to evaluate the characteristics of artificial caries lesions on the enamel surface, as well as preventive protocols. These procedures include scanning electron microscopy (SEM) (Elkassas & Arafa, 2014; Whittaker, 1982), microradiography, (Itthagarun et al, 2007; ten Cate & Duijsters, 1982), microhardness (Mohd Said, Ekambaram, & Yiu, 2017), polarized light microscopy (PLM) (Itthagarun et al, 2007; Itthagarun, Wei, & Wefel, 2000; Salman, ElTekeya, Bakry, Omar, & El Tantawi, 2019; Tuloglu, Bayrak, Tunc, & Ozer, 2016) and Energy dispersive X‐ray spectrometer (EDS), (De Menezes Oliveira et al, 2010); however, some of these methods require destructive sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Enamel demineralization model in primary teeth: Micro‐CT and SEM assessments of artificial incipient lesion

González-Sotelo

Contreras‐Bulnes

Rodríguez‐Vilchis

et al. 2021

Microscopy Res & Technique

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Many studies have analyzed different tooth demineralization models, which generate artificial incipient lesions; however, most of them are complex, slow, not clear and results could not be employed in both primary and permanent teeth because of chemical content differences among them. This study evaluates a demineralization model on primary enamel, under three incubation periods; quantifying artificial incipient lesions formation, and depth by micro‐CT, complementing with SEM for morphological characterization. Sixteen healthy human anterior primary teeth extracted for prolonged retention and orthopedic/orthodontic reasons were included in this study, previous informed consent. The sample was randomly assigned to four groups n = 4: G_Control, G_2D, G_4D, and G_7D. Micro‐CT and SEM were performed during two stages: before demineralization (BD) and after demineralization (AD). A t‐student test was carried out to determine differences among groups (p ≤ .05). No incipient lesions were observed in control group. Artificial lesion depth was similar among experimental groups; values were from 38.16 ± 05.40 μm to 42.61 ± 04.75 μm. An amount of 14 to 17 artificial incipient lesions were formed per group, the extension and distribution were different for each incubation period. Five erosive lesions were produced in G_7D. All experimental groups were able to form incipient artificial lesions in primary enamel. SEM characterization revealed more pronounced changes on the enamel surface, as the days of immersion in the demineralization solution increased. The 4‐day incubation period is the most recommended for the demineralization model, due to the formation of incipient lesions only and its extension, which facilitates their assessment.

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Comparison of remineralization by fluoride varnishes with and without casein phosphopeptide amorphous calcium phosphate in primary teeth

Abstract: MI varnish had better remineralization effect in primary teeth than Prevident varnish in terms of higher mineral content and shallower lesion depth.

Cited by 12 publications

References 24 publications

Fluoride release and remineralizing potential of varnishes in early caries lesions in primary teeth

Fluoride release and remineralizing potential of varnishes in early caries lesions in primary teeth

Incorporation of zwitterionic materials into light-curable fluoride varnish for biofilm inhibition and caries prevention

Enamel demineralization model in primary teeth: Micro‐CT and SEM assessments of artificial incipient lesion

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