Comparison of Real-time PCR versus ELISA in the diagnosis of cytomegalovirus infection in pregnant women

Sanousi, S.M. El; Osman, Zakia; Mohamed, Ahmed Babiker; Awfi, Mansoor; Babair, Yaser H.; Babair, Maher

doi:10.15761/cmid.1000114

Cited by 8 publications

(10 citation statements)

References 3 publications

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“…Out of 57 cases, 6 (10.53%) were positive for CMV IgM, 50 (87.72%) were positive for CMV IgG and 18 (31.58%) were positive for CMV DNA by PCR. In our study, out of 18 cases positive for CMV DNA, 6(33.34%) cases were having viral load 1x10 3 IU/ml, and 4(22.22%) cases each of having viral load 1x10 4 IU/ml, 1x10 5 IU/m and 1x10 6 IU/ ml respectively (Table 1). In our study, out of 50 CMV IgG seropositives, 15 cases were positive for CMV DNA by PCR and 35 cases were negative for CMV DNA ( Table 2).…”

Section: Resultsmentioning

confidence: 51%

“…15 A study conducted by EI Sanousi et al reported prevalence of CMV DNA 29.7% among pregnant women. 6 However, Aliyu et al reported detection of 45% CMV DNA among HIV seropositives. 16 Shukla et al reported prevalence of CMV DNA of 49% in India.…”

Section: Discussionmentioning

confidence: 99%

“…11 In contrast, Realtime PCR methods for CMV DNA quantitation offer a convenient alternative for monitoring CMV replication. 6 It also helps to diagnose active CMV infection as CMV DNA viral load showed a qualitative and quantitative correlation with the presence of symptoms. 1 The specific CMV IgM showed very little relationship with viral replication regarding active and recurrent infections, since it was positive in only 1 of the 18 cases of positive real time polymerase chain reaction for CMV.…”

Section: Discussionmentioning

confidence: 99%

“…IgM antibodies can also persist for a long time after infection in some healthy individuals. 6 Parmigiani et al reported that the accuracy of the serological tests for the diagnosis of CMV infection was lower than that of the polymerase chain reaction. 6,22 Seropevalence of CMV IgG in all age groups was generally high.…”

Section: Discussionmentioning

confidence: 99%

“…Optical Density of the samples were recorded on ELISA reader at the end of procedure. 6 CMV PCR: DNA extraction was done using spin column based method [QIAGEN QIAamp DNA Blood Mini Kit, Germany ] followed by detection and quantification of CMV by Real-time PCR (Fast-track Diagnostics kit, Luxemberg).…”

Section: Methodsmentioning

confidence: 99%

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Detection and Quantification of Cytomegalovirus in Immunocompromised Patients

Arora¹,

Devi²,

Singh³

et al. 2018

IJCMR

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Introduction: CMV disease ranges from asymptomatic infection in immunocompetent people to severe and lifethreatening infections in neonates and immunocompromised patients. The present study was undertaken to study the seroprevalence of CMV and use of Real-time PCR in detection and quantification of CMV in immunocompromised patients. Material and Methods: A total of 57 samples were tested for CMV IgM and CMV IgG by ELISA technique. The detection and quantification of CMV DNA was done by real time PCR. Results: Out of 57samples, 6 (10.53%) tested positive for CMV IgM, 50 (87.72%) were positive for CMV IgG and 18 (31.58%) were positive for CMV DNA by PCR. Among 18 PCR Positive cases, 6 (33.334%) cases showed viral load of 1x10 3 IU/ml. 4 (22.22%) cases each showed viral load of 1x10 4 IU/ml, 1x10 5 IU/ml, 1x10 6 IU/ml respectively. There is no statistical association of age, gender and geographical distribution with CMV IgM, CMV IgG and CMV DNA positivity. (P>0.05). Conclusion: This study demonstrated that Real-time PCR is more reliable test than serological ELISA test in the diagnosis of cytomegalovirus as Real-time PCR detects and quantifies viral DNA which is useful in predicting the patient's risk for disease and monitoring the effect of antiviral therapy. Good hygiene and infection control practices are advised to prevent CMV transmission.

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Section: Resultsmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Detection and Quantification of Cytomegalovirus in Immunocompromised Patients

Arora¹,

Devi²,

Singh³

et al. 2018

IJCMR

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Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children

Hassan

Zekry²,

Heikal³

et al. 2023

Parasitol Res

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Autism is a neurodevelopmental disorder with a significantly increased incidence rate across the world over the past few years. Toxoplasmosis and cytomegalovirus (CMV) infection are globally prevalent and have been associated with diverse neurological and psychiatric disorders. A few studies have demonstrated the role of toxoplasmosis and CMV as potential etiological factors for autism. Accordingly, this study was performed to estimate the relationship between toxoplasmosis and CMV infection in children with autism as well as to assess their impact on the Childhood Autism Rating Scale (CARS) score. A total of 45 autistic children (6 girls, 39 boys) and 45 (21 girls, 24 boys) healthy control children were enrolled in our study. Their blood samples were collected and tested for the presence of Toxoplasma and CMV (IgG and IgM) antibodies and DNA by ELISA and real-time PCR (RT-PCR), respectively. Toxoplasmosis was detected in 11 (24.4%) autistic children through the ELISA [10 (22.2%) IgG + /IgM − and 1 (2.2%) IgG + /IgM +]; however, RT-PCR assay recorded only 1 positive case (2.2%), while it was detected in 10 (22.2%) control children through ELISA [9 (20%) IgG + /IgM − and 1 (2.2%) IgG + /IgM +] and 1 (2.2%) by RT-PCR. On the other hand, CMV infection was detected in all autistic children with 44 (97.8%) testing positive by ELISA [24 (53.3%) IgG + /IgM − , 18 (40%) IgG + /IgM + and 2 (4.4%) IgG − /IgM +] and 25 (55.6%) testing positive by RT-PCR assay. In addition, ELISA assay recorded 43 (95.6%) [19 (42.2%) IgG + /IgM + and 22 (48.9%) IgG + /IgM − and 2 (4.4%) IgG-/IgM +] and RT-PCR recorded 21 (46.7%) positive samples in control children with CMV. No significant difference was noted between autistic and control children for the overall prevalence of Toxoplasma or/and CMV infection. Similarly, the CARS score indicated a non-significant difference with Toxoplasma or/and CMV infection. Our data does not show an association between autism and toxoplasmosis or/and CMV infection. Nevertheless, considering that autistic children are at a high risk of contracting these infections, further studies with a larger sample size are recommended.

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Development of a new plasmonic transducer for the detection of biological species

Laffont

Crespo‐Monteiro

Berini

et al. 2022

Optical Sensing and Detection VII

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During the COVID-19 outbreak, PCR tests were widely used for large-scale testing and screening.Yet, this technique requires bulky and time-consuming procedures to prepare the samples collected from the patients before their analysis by well-trained experts with expensive and specific equipment. PCR is therefore not competitive as a technique of detection for a widespread and rapid use in point-of-care sites. Thus, the COVID-19 pandemic highlighted the need for cheap and easy-to-implement biosensors. Surface plasmon resonance based sensors were suggested as a promising alternative in recent years. Indeed, they enable real-time and label-free detection of a wide range of analytes. That explains their widespread use in various fields of applications such as pharmacology, toxicology, food safety, and diagnosis. This thesis proposes and demonstrates a new plasmonic configuration of detection, which can address challenges posed by point-of-care settings. The gratings used as transducers in this configuration were fabricated based on laser interference lithography combined with a nanoimprinting process. The responses of these nanostructures interrogated by a p-polarized light beam result in a transfer of energy between two diffracted orders over an angular scan. This optical phenomenon termed as "optical switch", was theoretically and experimentally investigated and optimized.The principle of detection based on this specific configuration was demonstrated for the detection of small variations in the bulk refractive index with solutions comprised of different ratios v Abstract of de-ionized water and glycerol. A limit of detection in the range of 10 −6 RIU was achieved.In addition, preliminary bio-assays obtained by combining this configuration with a functionalization are presented and demonstrate the selectivity and the potential of this new plasmonic configuration for biosensing applications. This thesis work paves the way for the use of the optical switch configuration as a biosensor aligned with low-cost manufacturing and relevant for diagnosing in point-of-care sites. vi List of Figures 1.4 Sketches of the sequential steps for an ELISA assay consisting in detecting and quantifying gentamicin (red Y). A solution with a high gentamicin concentration results in a clearer solution than a solution with a low gentamicin concentration. That manifests through a lower (resp. higher) absorbance for high (resp. low) gentamicin concentrations, as shown in Figure 1.3. These sketches are extracted from [23].

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Comparison of Real-time PCR versus ELISA in the diagnosis of cytomegalovirus infection in pregnant women

Cited by 8 publications

References 3 publications

Detection and Quantification of Cytomegalovirus in Immunocompromised Patients

Detection and Quantification of Cytomegalovirus in Immunocompromised Patients

Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children

Development of a new plasmonic transducer for the detection of biological species

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