Detection and Quantification of Cytomegalovirus in Immunocompromised Patients

Arora, Tanu; Devi, Pushpa; Singh, Kanwardeep; Sidhu, Stanley; Oberoi, Loveena; Malhotra, Sita

doi:10.21276/ijcmr.2018.5.11.28

Cited by 2 publications

(4 citation statements)

References 17 publications

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“…In control children with CMV, ELISA recorded 43 (95.6%) [19 (42.2%) IgG + /IgM + , 22 (48.9%) IgG + /IgM − , and 2 (4.4%) IgG − /IgM +] and RT-PCR recorded 21 (46.7%) cases. Seropositivity of CMV antibodies was significantly higher by ELISA than by RT-PCR, which agrees with some data reported previously (El Sanousi et al 2016 ; Arora et al 2018 ). Our observation suggests that almost half of the enrolled autistic and control children had latent CMV infection with reactivation or active infection.…”

Section: Discussionsupporting

confidence: 92%

“…Furthermore, CMV infection in both autistic and control children showed a high incidence across different ages, as also reported previously by Arora et al ( 2018 ). In terms of gender, a significant difference was noted in CMV prevalence between boys and girls, with a higher rate in boys than in girls; however, it should be considered that the enrolled autistic children had more numbers of girls than boys.…”

Section: Discussionsupporting

confidence: 88%

“…Also, considering that most of the children from both sexes were affected, we cannot consider this data as the absolute significance and can consider it a bias. Meanwhile, a similar result has been observed by Seale et al ( 2006 ), Arora et al ( 2018 ), and Franjića et al ( 2020 ). In contrast, Adeiza et al ( 2016 ) mentioned higher rates of CMV-IgM seroprevalence in males, while Shukla et al ( 2015 ) and Bakri et al ( 2016 ) reported a higher incidence of CMV-IgG seroprevalence among females.…”

Section: Discussionsupporting

confidence: 86%

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Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children

Hassan

Zekry²,

Heikal³

et al. 2023

Parasitol Res

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Autism is a neurodevelopmental disorder with a significantly increased incidence rate across the world over the past few years. Toxoplasmosis and cytomegalovirus (CMV) infection are globally prevalent and have been associated with diverse neurological and psychiatric disorders. A few studies have demonstrated the role of toxoplasmosis and CMV as potential etiological factors for autism. Accordingly, this study was performed to estimate the relationship between toxoplasmosis and CMV infection in children with autism as well as to assess their impact on the Childhood Autism Rating Scale (CARS) score. A total of 45 autistic children (6 girls, 39 boys) and 45 (21 girls, 24 boys) healthy control children were enrolled in our study. Their blood samples were collected and tested for the presence of Toxoplasma and CMV (IgG and IgM) antibodies and DNA by ELISA and real-time PCR (RT-PCR), respectively. Toxoplasmosis was detected in 11 (24.4%) autistic children through the ELISA [10 (22.2%) IgG + /IgM − and 1 (2.2%) IgG + /IgM +]; however, RT-PCR assay recorded only 1 positive case (2.2%), while it was detected in 10 (22.2%) control children through ELISA [9 (20%) IgG + /IgM − and 1 (2.2%) IgG + /IgM +] and 1 (2.2%) by RT-PCR. On the other hand, CMV infection was detected in all autistic children with 44 (97.8%) testing positive by ELISA [24 (53.3%) IgG + /IgM − , 18 (40%) IgG + /IgM + and 2 (4.4%) IgG − /IgM +] and 25 (55.6%) testing positive by RT-PCR assay. In addition, ELISA assay recorded 43 (95.6%) [19 (42.2%) IgG + /IgM + and 22 (48.9%) IgG + /IgM − and 2 (4.4%) IgG-/IgM +] and RT-PCR recorded 21 (46.7%) positive samples in control children with CMV. No significant difference was noted between autistic and control children for the overall prevalence of Toxoplasma or/and CMV infection. Similarly, the CARS score indicated a non-significant difference with Toxoplasma or/and CMV infection. Our data does not show an association between autism and toxoplasmosis or/and CMV infection. Nevertheless, considering that autistic children are at a high risk of contracting these infections, further studies with a larger sample size are recommended.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children

Hassan

Zekry²,

Heikal³

et al. 2023

Parasitol Res

View full text Add to dashboard Cite

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“…On the other hand, other studies reported that CMV IgM in blood donors with positivity of 2.3%, 1.6%, and 0.07% from Souza, Delhi, Pune by (Souza et al, 2010;Souza et al, 1996;Kumar et al, 2008) respectively. These differences in IgM rates could be related to factors such as assay methods, sample size, geographic distribution and socioeconomic status (Arora, et al 2018).…”

Section: Antibody Positive To Both Igg Andmentioning

confidence: 99%

Seroprevalence of Cytomegalovirus Among Voluntary Blood Donors in Erbil Province, North Iraq.

2019

ZJPAS

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Background and objectives: Cytomegalovirus (CMV) is one of the most significant agent of infection in immunocompromised patients and transplant recipients. CMV is transmissible through transfusions of blood components. The present study amied to determine the seroprevalence of Cytomegalovirus (CMV) among voluntary blood donors in Erbil province, North of Iraq. Methods: A prospective study was done on serum samples from 472 HIV, HCV, and HbsAg-seronegative healthy male voluntary blood donors were screened for CMV IgM /IgG using Enzyme-Linked Immunosorbent assay (ELISA). Results: A total of 472 healthy voluntary blood donors were recruited; 148(31.36%) of the donors were anti-CMV IgG positive. There were no statistically significant differences between CMV seropositivity and age. Further, seven (1.48%) subjects were both anti-CMV IgM and IgG positive. Conclusions: The moderate seroprevalence of CMV IgG antibodies in the our donor population, underscores the importance of using strategies such as leukoreduction and transfusion with CMV-seronegative blood is recommended for preventing and reducing transmission of CMV with the infected blood or blood products in immunocompromised patients.

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Detection and Quantification of Cytomegalovirus in Immunocompromised Patients

Cited by 2 publications

References 17 publications

Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children

Toxoplasmosis and cytomegalovirus infection and their role in Egyptian autistic children

Seroprevalence of Cytomegalovirus Among Voluntary Blood Donors in Erbil Province, North Iraq.

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