Comparison of raw and processed Radix Polygoni Multiflori (Heshouwu) by high performance liquid chromatography and mass spectrometry

Abstract

BackgroundRadix Polygoni Multiflori is the dried root tuber of Polygonum multiflorum Thunb. (Fam. Polygonaceae). According to Chinese medicine theory, raw (R-RPM) and processed (P-RPM) Radix Polygoni Multiflori possess different properties. The present study investigates the differences in chemistry between raw and processed Radix Polygoni Multiflori.MethodsFive pairs of R-RPM and P-RPM as well as 15 commercial decoction pieces were analyzed with high performance liquid chromatography (HPLC) and mass spectrometry (MS).ResultsTwo anthraquinones, namely emodin-8-O-(6'-O-malonyl)-glucoside and physcion-8-O-(6'-O-malonyl)-glucoside disappeared or decreased significantly and 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucopyranoside, emodin-8-O-β-D-glucopyranoside and physcion-8-O-β-D-glucopyranoside decreased after the R-RPM samples being processed. On the other hand, the contents of emodin and physcion generally increased after processing.ConclusionThe present study indicates that processing Radix Polygoni Multiflori may change the contents and types of chemicals in it. These changes are probably responsible for the various pharmacological effects of R-RPM and P-RPM as well as hepatotoxicity.

A systematic review and meta-analysis of traditional insect Chinese medicines combined chemotherapy for non-surgical hepatocellular carcinoma therapy

“…ComplicationLiang B.L. 48 2010202030-OctE:59 ± 6 years old—— ≥ 50Sodium Cantharidinate and vitamin B6 + TACE (MMC, 5-FU, EPI)TACE (MMC, 5-FU, EPI)4weeksComparison of liver function and blood routine examinationC:55 ± 8 years oldLiang C.X. 49 2015303034/26E:median age 53 years—A and B>80Sodium Cantharidinate and Vitamin B6 + TACE (5-FU, DDP, OXA, EPI)TACE (5-FU, DDP, OXA, EPI)3–4 rounds (15 days per round)1.…”

Integrating Targeted and Untargeted Metabolomics to Investigate the Processing Chemistry of Polygoni Multiflori Radix

“…One tenth of each group were reserved as materials of RPMR; the rest was subsequently processed. To fix the specific factors involved in the processing, reports in the literature (Li, 2008; Liang et al, 2010; Liu et al, 2011; Chen et al, 2016; Wang et al, 2016) and the Chinese pharmacopeia (2015 edition) (Chinese Pharmacopoeia Commission, 2015) were referenced. Finally, 4 h steaming with black bean (put one layer of water-soaked black beans and one layer of RPMR slices in a pot, repeat the layers, and then steam over boiling water) and then 24 h sun-drying, repeated for a total of 9 cycles, were used as processing conditions.…”

“…By quantifying these secondary metabolites using liquid chromatography coupled with diode-array detectors or mass spectrometry (LC-DAD/MS), some previous studies investigated the processing chemistry of PMR. The results showed that processing indeed changed the contents of certain chemicals in PMR (Liang et al, 2010; Liu et al, 2011; Chen et al, 2016; Wang et al, 2016). For example, we previously found that the contents of two anthraquinones (emodin and physicon) in PMR were increased by the processing, while one stilbene glucoside named 2,3,5,4′-tetrahydroxystilbene-2- O -β-D-glucoside (THSG) was decreased (Liang et al, 2010).…”

“…Firstly, they selected several typical chemical components for the determination, and did not characterize the entire chemical profile of PMR. Secondly, and more importantly, these studies analyzed PPMR that had been processed by only one cycle of steaming and drying (Liang et al, 2010; Liu et al, 2011; Chen et al, 2016; Wang et al, 2016), which is different from the traditional processing technology of PMR which stipulates nine cycles of steaming and sun-drying (Li, 2008). Given these facts, further research is needed to fully elucidate the processing chemistry of PMR.…”

Hepatotoxicity in Rats Induced by Aqueous Extract of Polygoni Multiflori Radix, Root of Polygonum multiflorum Related to the Activity Inhibition of CYP1A2 or CYP2E1

“…All tested solutions were filtered through 0.45  μ m membrane syringe filters before use. The method followed [30], and the calibration curve was established for the standards: the concentration range of THSG from 46 to 920  μ g/mL ( Y = 29722 × X − 125033, r 2 = 0.9998), the concentration range of EG from 1.04 to 41.6  μ g/mL ( Y = 52836 × X + 12471, r 2 = 0.9992), the concentration range of emodin from 0.273 to 7.8  μ g/mL ( Y = 66516 × X + 9285, r 2 = 0.9996), and the concentration range of emodin methyl ether from 0.111 to 28.86  μ g/mL ( Y = 67576 × X − 313, r 2 = 0.9998), and the measurement accuracy and precision are favorable, RSD < 5%.…”