Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite

Tobler, Dominique J.; Cuthbert, Mark O.; Greswell, R.; Riley, Michael S.; Renshaw, Joanna C.; Handley-Sidhu, Stephanie; Phoenix, Vernon R.

doi:10.1016/j.gca.2011.03.023

Cited by 159 publications

(163 citation statements)

References 28 publications

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“…Biostimulation, in contrast, encourages the growth of a particular guild of native soil microfauna through the manipulation of specific growth conditions. However, a possible drawback of this method is that the initial soil concentration of ureolytic bacteria might limit the rate of ureolytic MICP in the site to be treated (Tobler et al, 2011). Another consideration affecting biostimulation is the cellular regulation over urease expression.…”

Section: Co(nhmentioning

confidence: 99%

“…Although some studies of ureolytic MICP have been conducted using mixed bacterial cultures in the lab (De Muynck et al, 2008a;Tobler et al, 2011) and others have been conducted in situ by stimulation of indigenous ureolytic bacteria (Burbank et al, 2011;Fujita et al, 2008), little attention has been paid to the effect on the system of the non-ureolytic bacteria present in the experimental setting. In this study, the potentially conflicting effects of ureolytic and non-ureolytic bacteria on CaCO 3 precipitation were investigated in a simple, two-species batch experiment.…”

Section: Co(nhmentioning

confidence: 99%

“…In this study we showed that the presence of the nonureolytic bacterium, B. subtilis, had no effect on the precipitation process when the concentration of NB (i.e., organic carbon source) was low (1/3 NBps treatment), but increased the rate of CaCO 3 precipitation when the NB concentration was high (NBps treatment). Since most studies of MICP in natural soils have used simple organic carbon sources, e.g., molasses, at low concentrations (Fujita et al, 2008;Burbank et al, 2011;Tobler et al, 2011), the results for the 1/3 NBps treatment are particularly relevant to the scaling up of MICP.…”

Section: Growth Conditions and Bacterial Growthmentioning

confidence: 99%

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Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Gat

Tsesarsky

Shamir³

et al. 2014

Biogeosciences

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Abstract. Microbial-induced CaCO 3 precipitation (MICP) is an innovative technique that harnesses bacterial activity for the modification of the physical properties of soils. Since stimulation of MICP by urea hydrolysis in natural soils is likely to be affected by interactions between ureolytic and non-ureolytic bacteria, we designed an experiment to examine the interactions between ureolytic and non-ureolytic bacteria and the effect of these interactions on MICP. An artificial groundwater-based rich medium was inoculated with two model species of bacteria, the ureolytic species Sporosarcina pasteurii and the non-ureolytic species Bacillus subtilis. The control treatment was inoculated with a pure culture of S. pasteurii. The following parameters were monitored during the course of the experiment: optical density, pH, the evolution of ammonium, dissolved calcium and dissolved inorganic carbon. The results showed that dissolved calcium was precipitated as CaCO 3 faster in the mixed culture than in the control, despite less favorable chemical conditions in the mixed culture, i.e., lower pH and lower CO 2− 3 concentration. B. subtilis exhibited a considerably higher growth rate than S. pasteurii, resulting in higher density of bacterial cells in the mixed culture. We suggest that the presence of the non-ureolytic bacterial species, B. subtilis, accelerated the MICP process, via the supply of nucleation sites in the form of non-ureolytic bacterial cells.

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Section: Co(nhmentioning

confidence: 99%

Section: Co(nhmentioning

confidence: 99%

Section: Growth Conditions and Bacterial Growthmentioning

confidence: 99%

See 1 more Smart Citation

Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Gat

Tsesarsky

Shamir³

et al. 2014

Biogeosciences

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“…For a large macro-pore, and rate of hydrolysis proportional to the urea concentration (e.g. Tobler et al, 2011), the steady-state flux of urea into the soil is approximately (Crank, 1975, Eq. 4.50): where [urea] 0 is the concentration in the macro-pore, D U the urea diffusion coefficient in the soil and k the hydrolysis rate constant.…”

Section: Sensitivity Analysis B: For Practical Applications In Cylindmentioning

confidence: 99%

“…If CaCO 3 precipitates in the zone of pH increase, this will curb the pH rise and thereby reduce the losses of NH 3 . There is currently particular interest in exploiting microbiallyenhanced urea hydrolysis in soils and sub-strata to stimulate CaCO 3 precipitation for various applications such as physical stabilisation of soils (Stocks-Fischer et al, 1999;Chu et al, 2012), capture of heavy metals and radionuclides (Mitchell and Ferris., 2005;Fujita et al, 2010;Tobler et al, 2011), sealing of leaks in CO 2 storage reservoirs (Ferris et al, 1996;Cunningham et al, 2009), and carbon sequestration (Dupraz et al, 2009;Mitchell et al, 2010;Renforth et al, 2009;Whitmore et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

A simple reactive-transport model of calcite precipitation in soils and other porous media

Kirk

Versteegen

Ritz

et al. 2015

Geochimica et Cosmochimica Acta

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Calcite formation in soils and other porous media generally occurs around a localised source of reactants, such as a plant root or soil macro-pore, and the rate depends on the transport of reactants to and from the precipitation zone, as well as the kinetics of the precipitation reaction itself. However most studies are conducted using well-mixed systems, in which such transport limitations are largely removed. We developed a mathematical model of calcite precipitation near a source of base in soil, allowing for transport limitations and precipitation kinetics. We tested the model against experimentally-determined rates of calcite precipitation and reactant concentration:distance profiles in columns of soil in contact with a layer of HCO 3 --saturated exchange resin. The model parameter values were determined independently. The agreement between observed and predicted results was satisfactory given experimental limitations, indicating that the model correctly describes the important processes. A sensitivity analysis showed that all model parameters are important, indicating a simpler treatment would be inadequate. The sensitivity analysis showed that the amount of calcite precipitated and the spread of the precipitation zone were sensitive to parameters controlling rates of reactant transport (soil moisture content, salt content, pH, pH buffer power and CO 2 pressure), as well as to the precipitation rate constant. We illustrate practical applications of the model with two examples, viz. the effect of pH changes and CaCO 3 precipitation in the soil around a plant root, and around a soil macro-pore containing a source of base such as urea.

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Hydrodynamic coupling in microbially mediated fracture mineralization: Formation of self-organized groundwater flow channels

et al. 2014

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[1] Evidence of fossilized microorganisms embedded within mineral veins and mineralfilled fractures has been observed in a wide range of geological environments. Microorganisms can act as sites for mineral nucleation and also contribute to mineral precipitation by inducing local geochemical changes. In this study, we explore fundamental controls on microbially induced mineralization in rock fractures. Specifically, we systematically investigate the influence of hydrodynamics (velocity, flow rate, and aperture) on microbially mediated calcite precipitation. Our experimental results demonstrate that a feedback mechanism exists between the gradual reduction in fracture aperture due to precipitation, and its effect on the local fluid velocity. This feedback results in mineral-fill distributions that focus flow into a small number of self-organizing channels that remain open, ultimately controlling the final aperture profile that governs flow within the fracture. This hydrodynamic coupling can explain field observations of discrete groundwater flow channeling within fracture-fill mineral geometries where strong evidence of microbial activity is reported.

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Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite

Cited by 159 publications

References 28 publications

Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

A simple reactive-transport model of calcite precipitation in soils and other porous media

Hydrodynamic coupling in microbially mediated fracture mineralization: Formation of self-organized groundwater flow channels

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Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite

Cited by 159 publications

References 28 publications

Accelerated microbial-induced CaCO&lt;sub&gt;3&lt;/sub&gt; precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Accelerated microbial-induced CaCO&lt;sub&gt;3&lt;/sub&gt; precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

A simple reactive-transport model of calcite precipitation in soils and other porous media

Hydrodynamic coupling in microbially mediated fracture mineralization: Formation of self-organized groundwater flow channels

Contact Info

Product

Resources

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Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria

Accelerated microbial-induced CaCO<sub>3</sub> precipitation in a defined coculture of ureolytic and non-ureolytic bacteria