Comparison of Quantitative Characteristics of Early Post-resuscitation EEG Between Asphyxial and Ventricular Fibrillation Cardiac Arrest in Rats

Chen, Bihua; Chen, Gang; Dai, Chenxi; Wang, Pei; Zhang, Lei; Huang, Yuanyuan; Li, Yongqin

doi:10.1007/s12028-017-0401-z

Cited by 16 publications

(17 citation statements)

References 34 publications

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“…To ensure normothermia (36.5 –37.5°C), a heat lamp was operated following the core temperature monitored by a thermocouple sensor (TH212, Bjhocy Science and Technology Co. Ltd., Beijing, China) that was placed into the esophagus. A couple of subdermal needles were inserted into both left and right surfaces of the skull respectively to record the EEG signals [14]. To prevent clotting in catheters, they were flushed intermittently with saline containing 2.5 IU/ml crystalline bovine heparin.…”

Section: Methodsmentioning

confidence: 99%

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Inhaling Hydrogen Ameliorates Early Postresuscitation EEG Characteristics in an Asphyxial Cardiac Arrest Rat Model

Chen

Wang

et al. 2019

BioMed Research International

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Background Electroencephalography (EEG) is commonly used to assess the neurological prognosis of comatose patients after cardiac arrest (CA). However, the early prognostic accuracy of EEG may be affected by postresuscitation interventions. Recent animal studies found that hydrogen inhalation after CA greatly improved neurological outcomes by selectively neutralizing highly reactive oxidants, but the effect of hydrogen inhalation on EEG recovery and its prognostication value are still unclear. The present study investigated the effects of hydrogen inhalation on early postresuscitation EEG characteristics in an asphyxial CA rat model. MethodsCardiopulmonary resuscitation was initiated after 5 min of untreated CA in 40 adult female Sprague-Dawley rats. Animals were randomized for ventilation with 98% oxygen plus 2% hydrogen (H2) or 98% oxygen plus 2% nitrogen (Ctrl) under normothermia for 1 h. EEG characteristics were continuously recorded for 4 h, and the relationships between quantitative EEG characteristics and 96 h neurological outcomes were investigated. ResultsNo differences in baseline and resuscitation data were observed between groups, but the survival rate was significantly higher in the H2 group than in the Ctrl group (90% vs. 40%, P < 0.01). Compared to the Ctrl group, the H2 group showed a shorter burst onset time (21.85 [20.00–23.38] vs. 25.70 [22.48–30.05], P < 0.01) and time to normal trace (169.83 [161.63–208.55] vs. 208.39 [186.29–248.80], P < 0.01). Additionally, the burst suppression ratio (0.66 ± 0.09 vs. 0.52 ± 0.17, P < 0.01) and weighted‐permutation entropy (0.47 ± 0.16 vs. 0.34 ± 0.13, P < 0.01) were markedly higher in the H2 group. The areas under the receiver operating characteristic curves for the 4 EEG characteristics in predicting survival were 0.82, 0.84, 0.88, and 0.83, respectively. ConclusionsIn this asphyxial CA rat model, the improved postresuscitation EEG characteristics for animals treated with hydrogen are correlated with the better 96 h neurological outcome and predicted survival.

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Section: Methodsmentioning

confidence: 99%

“…The CA was induced as previously descripted [14]. Briefly, after collection of baseline data and removal of the thermocouple sensor, the mechanical ventilator was turned off, and the endotracheal tube was clamped to induce asphyxia.…”

Section: Methodsmentioning

confidence: 99%

Inhaling Hydrogen Ameliorates Early Postresuscitation EEG Characteristics in an Asphyxial Cardiac Arrest Rat Model

Chen

Wang

et al. 2019

BioMed Research International

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“…24-hr NDS was unable to be performed on Cohort 3, so 90 min post-ROSC ECoG information quantity (IQ) was used to measure short-term neurological outcome following resuscitation. 49…”

Section: Preclinical Modelmentioning

confidence: 99%

“…For Cohort 3, the median ECoG IQ from 90 to 100 min post-ROSC was calculated from WB ECoG, as previously described. 49 The baseline microstates were determined from the final minute of anesthesia washout prior to start of asphyxia. AC-ECoG IQ was also normalized to this baseline period.…”

Section: Ac/dc-ecogmentioning

confidence: 99%

Spreading depolarization and repolarization during cardiac arrest as an ultra-early marker of neurological recovery in a preclinical model

Wilson¹,

Crouzet²,

Lee

et al. 2019

Preprint

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Spreading depolarization (SD) accompanies numerous neurological conditions, including migraine, stroke, and traumatic brain injury. There is significant interest in understanding the relationship between SD and neuronal injury. However, characteristics underlying SD and repolarization (RP) induced by global cerebral ischemia (e.g., cardiac arrest (CA)) and reperfusion are not well understood. Quantifying features of SD and RP during CA and cardiopulmonary resuscitation (CPR) may provide important metrics for diagnosis and prognosis of neurological injury from hypoxia-ischemia. We characterized SD and RP in a rodent model of asphyxial CA+CPR using a multimodal platform including electrocorticography (ECoG) and optical imaging. We detected SD and RP by (1) alternating current (AC), (2) direct current (DC), and (3) optical imaging of spreading ischemia, spreading edema, and vasoconstriction. Earlier SD (r=-0.80; p<0.001) and earlier RP (r=-0.71, p<0.001) were associated with better neurological recovery after 24hrs. SD+RP onset times predicted good vs poor neurological recovery with 82% sensitivity and 91% specificity. To our knowledge, this is the first preclinical study to link SD and RP characteristics with neurological recovery post-CA. These data suggest that SD and RP may be ultra-early, real-time prognostic markers of post-CA outcome, meriting further investigation into translational implications during global cerebral ischemia.

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“…Recovery of ECoG signal following ROSC was quantified by (1) time to initial resumption (burst) of ECoG activity, and (2) ECoG Information Quantity (IQ) 90 min post-ROSC (as in [15]).…”

Section: Cardiac Arrest (Ca) and Cardiopulmonary Resuscitation (Cpr)mentioning

confidence: 99%

High-speed quantitative optical imaging of absolute metabolism in the rat cortex

Wilson

Crouzet

Torabzadeh

et al. 2019

Preprint

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Quantitative measures of blood flow and metabolism are essential for improved assessment of brain health and response to ischemic injury. In this report, we demonstrate a multimodal technique for measuring the cerebral metabolic rate of oxygen (CMRO 2 ) in the rodent brain on an absolute scale (M O 2 / min). We use laser speckle imaging (LSI) at 809 nm and spatial frequency domain imaging (SFDI) at 655 nm, 730 nm, and 850 nm to obtain spatiotemporal maps of cerebral blood flow (CBF), tissue absorption ( a ), and tissue scattering ( s '). Knowledge of these three values enables calculation of a characteristic blood flow speed, which in turn is input to a mathematical model with a "zero-flow" boundary condition to calculate absolute CMRO 2 . We apply this method to a rat model of cardiac arrest (CA) and cardiopulmonary resuscitation. With this model, the zero-flow condition occurs during entry into CA. The CMRO 2 values calculated with our method are in good agreement with those measured with magnetic resonance (MR) and positron emission tomography (PET) by other groups. Our technique provides a quantitative metric of cerebral metabolism that can potentially be used for comparison between animals and longitudinal monitoring of a single animal over multiple days, to assess differences in baseline metabolism and track recovery of metabolism in survival studies following ischemia and reperfusion.

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Comparison of Quantitative Characteristics of Early Post-resuscitation EEG Between Asphyxial and Ventricular Fibrillation Cardiac Arrest in Rats

Abstract: Characteristics of earlier post-resuscitation EEG differed between cardiac and respiratory causes. Quantitative measures of EEG not only predicted neurological outcome and survival, but also have the potential to stratify CA with different causes.

Cited by 16 publications

References 34 publications

Inhaling Hydrogen Ameliorates Early Postresuscitation EEG Characteristics in an Asphyxial Cardiac Arrest Rat Model

Inhaling Hydrogen Ameliorates Early Postresuscitation EEG Characteristics in an Asphyxial Cardiac Arrest Rat Model

Spreading depolarization and repolarization during cardiac arrest as an ultra-early marker of neurological recovery in a preclinical model

High-speed quantitative optical imaging of absolute metabolism in the rat cortex

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