Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and enterococci isolates from the feces of livestock and livestock farmers in Japan

Katsunuma, Yu; Hanazumi, Michihito; Fujisaki, Hirokazu; Minato, Hajime; Kataoka, Yuki; Sawada, T.; Hashimoto, Yasushi; Yonemochi, Chisato

doi:10.2323/jgam.54.39

Cited by 8 publications

(5 citation statements)

References 37 publications

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“…This phenomenon may confirm the high discriminatory potential of ADSRRS-fingerprinting method allowing for diversification of strains with the same or similar phenotypic resistance profile, but differing in relation to the genes that determine this resistance [ 12 ]. A similar regularity in the consistency of genetic profiles (obtained with the PFGE method) with resistance profiles was observed by other authors [ 6 , 7 , 32 , 42 , 57 ]. This regularity seems to be especially important for analysis of strains with similar or the same antibiograms obtained from different sources (different farms, flocks, or individuals) and for tracking the ways of potential spread of multidrug resistant strains or strains causing local outbreaks.…”

Section: Discussionsupporting

confidence: 87%

“…Many different methods have been used so far for typing Enterococcus bacteria in terms of their phenotypic and genotypic resistance [ 6 – 10 ]. However, most of the strains analyzed had a specific resistance profile, mainly including vancomycin- or high-level aminoglycoside-resistance, as groups with the greatest epidemiological significance.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Multidrug Resistant E. faecalis Strains from Pigs of Local Origin by ADSRRS-Fingerprinting and MALDI -TOF MS; Evaluation of the Compatibility of Methods Employed for Multidrug Resistance Analysis

Nowakiewicz

Ziółkowska

Zięba³

et al. 2017

PLoS ONE

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The aim of this study was to characterize multidrug resistant E. faecalis strains from pigs of local origin and to analyse the relationship between resistance and genotypic and proteomic profiles by amplification of DNA fragments surrounding rare restriction sites (ADSRRS-fingerprinting) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI -TOF MS). From the total pool of Enterococcus spp. isolated from 90 pigs, we selected 36 multidrug resistant E. faecalis strains, which represented three different phenotypic resistance profiles. Phenotypic resistance to tetracycline, macrolides, phenicols, and lincomycin and high-level resistance to aminoglycosides were confirmed by the occurrence of at least one corresponding resistance gene in each strain. Based on the analysis of the genotypic and phenotypic resistance of the strains tested, five distinct resistance profiles were generated. As a complement of this analysis, profiles of virulence genes were determined and these profiles corresponded to the phenotypic resistance profiles. The demonstration of resistance to a wide panel of antimicrobials by the strains tested in this study indicates the need of typing to determine the spread of resistance also at the local level. It seems that in the case of E. faecalis, type and scope of resistance strongly determines the genotypic pattern obtained with the ADSRRS-fingerprinting method. The ADSRRS-fingerprinting analysis showed consistency of the genetic profiles with the resistance profiles, while analysis of data with the use of the MALDI- TOF MS method did not demonstrate direct reproduction of the clustering pattern obtained with this method. Our observations were confirmed by statistical analysis (Simpson’s index of diversity, Rand and Wallace coefficients). Even though the MALDI -TOF MS method showed slightly higher discrimination power than ADSRRS-fingerprinting, only the latter method allowed reproduction of the clustering pattern of isolates based on phenotypic resistance and analysis of resistance and virulence genes (Wallace coefficient 1.0). This feature seems to be the most useful for epidemiological purposes and short-term analysis.

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Section: Discussionsupporting

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Characterization of Multidrug Resistant E. faecalis Strains from Pigs of Local Origin by ADSRRS-Fingerprinting and MALDI -TOF MS; Evaluation of the Compatibility of Methods Employed for Multidrug Resistance Analysis

Nowakiewicz

Ziółkowska

Zięba³

et al. 2017

PLoS ONE

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“…Risk assessment studies have shown that chicken contributed more risk than other animal products in transmitting resistant enterococci to humans (Presi et al 2009). However, recent pulsed field gel electrophoresis studies showed that transmission of resistant enterococci from animal sources to humans or vice versa were less common (Katsunuma et al 2008).…”

Section: Discussionmentioning

confidence: 99%

Distribution of drug resistance among enterococci and Salmonella from poultry and cattle in Ethiopia

Bekele

Ashenafi

2009

Trop Anim Health Prod

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Enterococci and Salmonella were isolated from feces of chicken in intensive poultry farms and cattle which are maintained following traditional practices. Their resistance to different antibiotics was also determined. A total of 298 enterococcal isolates consisting of Enterococcus faecium (49.6%), Enterococcus durans (26.9%), Enterococcus hirea (11.9%), and Enterococcus faecalis (11.5%) were obtained. Among the enterococci, resistance to erythromycin (Ery), clindamicin (Cli), amoxicillin (Amo), ampicillin (Amp), and cephalothin (Cep) was high. Resistance to vancomycin (Van) was detected in all enterococcal species. Over 80% of the isolates showed multiple drug resistance. The most dominant patterns in poultry were Amo/Amp/Cep/Pen and Amo/Amp/Cep/Cli/Pen/Van. Among isolates from cattle, Amo/Amp/Cep/Cli/Ery/Pen/Van and Amo/Amp/Cli/Ery/Pen/Van constituted the most dominant multiple resistance patterns. A total of 51 Salmonella isolates were obtained from poultry (43/280) and cattle (8/450). About 70% of the isolates had varying resistance to the tested antibiotics. Multiple drug resistance was observed in over 30% of the Salmonella isolates. The most frequent resistance pattern was Amo/Amp/Cip/Gen/Str in cattle and Amo/Amp/Cep/Cip/Gen/Kan/Str in poultry. Enteroccoccal and Salmonella isolates showed multiple resistance to those antibiotics used in human and veterinary medicine. The high frequency of isolation of resistant enterococci is indicative of the wide dissemination of antibiotic resistant bacteria in the farm environment.

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“…Bactérias multirresistentes a drogas isoladas de humanos são geralmente clones dispersos no ambiente, em animais e vegetais que carreiam genes para resistência aos principais antibióticos de uso clínico. A utilização dos antimicrobianos gerou grande otimismo em relação à prevenção e ao tratamento dos processos infecciosos, entretanto, o uso exagerado e nem sempre criterioso e racional dos antibióticos e quimioterápicos trouxe problemas à Saúde Pública, sendo o maior deles representado pela progressiva resistência bacteriana às drogas [14,15] .…”

Section: Mesmounclassified

Qualidade microbiológica e caracterização da resistência antimicrobiana de bactérias isoladas de queijos Coalho comercializados em Vitória da Conquista-Bahia

Bomfim

Costa

Silva

et al. 2020

Segur. Aliment. Nutr.

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Neste estudo foram determinadas a qualidade higiênico-sanitária, a prevalência e a resistência antimicrobiana de patógenos isolados de 24 amostras de queijo Coalho comercializados em duas feiras livres do município de Vitória da Conquista, Bahia. Foram realizadas análises físico-químicas e microbiológicas através de métodos convencionais interpretados conforme legislação vigente. Identificamos que 75% das amostras eram comercializadas em temperatura inadequada e que 100% apresentaram contagem para coliformes totais acima do limite aceitável. Staphylococcus coagulase positiva e Escherichia coli foram isolados em 87,5% e 62,5% das amostras, respectivamente. Não foi detectada a presença de Listeria monocytogenes e Salmonella sp. Identificamos 51,1% de resistência a pelo menos um dos sete antimicrobianos testados para Staphylococcus coagulase positiva e 44,4% para pelo menos um dos 11 avaliados para E.coli. As análises realizadas evidenciaram uma precariedade higiênico-sanitária na produção e comercialização de queijo coalho nas feiras livres de Vitória da Conquista. Torna-se também uma preocupação, a presença de bactérias resistentes aos antimicrobianos nestes queijos. Deste modo, ressaltamos a importância do controle de qualidade na produção e comercialização destes produtos, assim como a necessidade das ações da vigilância sanitária para orientação aos manipuladores e produtores de queijo.

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Comparison of pulsed-field gel electrophoresis patterns of antimicrobial-resistant Escherichia coli and enterococci isolates from the feces of livestock and livestock farmers in Japan

Cited by 8 publications

References 37 publications

Characterization of Multidrug Resistant E. faecalis Strains from Pigs of Local Origin by ADSRRS-Fingerprinting and MALDI -TOF MS; Evaluation of the Compatibility of Methods Employed for Multidrug Resistance Analysis

Characterization of Multidrug Resistant E. faecalis Strains from Pigs of Local Origin by ADSRRS-Fingerprinting and MALDI -TOF MS; Evaluation of the Compatibility of Methods Employed for Multidrug Resistance Analysis

Distribution of drug resistance among enterococci and Salmonella from poultry and cattle in Ethiopia

Qualidade microbiológica e caracterização da resistência antimicrobiana de bactérias isoladas de queijos Coalho comercializados em Vitória da Conquista-Bahia

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