Comparison of protein A affinity sorbents

High Throughput Screening Techniques in Protein Purification

“…In addition, considering the intrinsic hardware limitations of a typical automated chromatography system that does not allow the performance of many experiments in parallel, the time factor associated with the development of the chromatography step was also significant. For instance, in order to develop a relatively simple capture step using a 1 mL column packed with a chromatography resin having a modest binding capacity, as much as 0.5 g of a valuable sample could be required, and the time for completion of such a study could be up to 5 days (16,17). The low throughput could be considered unacceptable, especially in industrial settings where process development groups are working with multiple processes simultaneously.…”

Downstream Processing of Plasmid DNA for Gene Therapy and Genetic Vaccination

“…In the case of protein purification, immobilized metal chelate affinity methods [54] as well as protein A chromatography [55] for the purification of monoclonal antibodies are wellknown techniques. Affinity interactions have also been used for plasmid purification.…”

Optimization and Performance of Silica‐Based Media for Industrial‐Scale Antibody Purification

“…In order to attain high-throughput purification of monoclonal antibodies on a large scale, the protein A-coupled media must have a high adsorption capacity, a high mass transfer rate for IgG, and high mechanical strength. However, protein A media made of cross-linked agarose, which are often used in laboratory-and industrial-scale production, possess a high static binding capacity and low nonspecific adsorption, but a relatively lower mechanical strength and mass transfer rate [3,4]. Silica matrices, however, are characterized by high mechanical strength and are commonly used in industrial-scale chromatographic separation [5].…”