Comparison of Procedures for Optimum Extraction. Purification and Analysis of Desulfo Indolyl Glucosinolates

Buchner, Reimar

doi:10.1007/978-94-009-3615-7_7

Cited by 8 publications

(4 citation statements)

References 2 publications

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“…The glucosinolates sinigrin, gluconapin, glucoraphenin, dehydroerucin, glucotropeolin, sinalbin, and those from rapeseed were isolated and purified according to the method proposed by Thies (1988) with some important modifications reported by Visentin et al (1992), starting from the seed or specified plant organ of some crucifers (Table I). The HPLC analyses of the desulfoderivates, conducted according to the method of Buchner (1986), showed the predominance of a single glucosinolate with a purity, solid weight, ranging from 66 to 89 %, except for the rapeseed extracts, which contained a mixture of glucosinolates containing ca. 60% progoitrin, 20% gluconapin, and other minor glucosinolates: epiprogoitrin, 4-hydroxyglucobrassicin, gluconasturtin, gluconapoleipherin, and others (Figure 2).…”

Section: Methodsmentioning

confidence: 99%

In vitro activity of some glucosinolates and their reaction products toward a population of the nematode Heterodera schachtii

Lazzeri¹,

Tacconi²,

Palmieri³

1993

J. Agric. Food Chem.

149

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Section: Methodsmentioning

confidence: 99%

In vitro activity of some glucosinolates and their reaction products toward a population of the nematode Heterodera schachtii

Lazzeri¹,

Tacconi²,

Palmieri³

1993

J. Agric. Food Chem.

149

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“…Glucosinolates were extracted from the roots of greenhouse-grown mustards by boiling fresh roots in 80% methanol for 10 min followed by grinding of the tissue with a mortar and pestle (Buchner, 1987). The methanol was removed by rotary evaporation.…”

Section: Methodsmentioning

confidence: 99%

Antifungal compounds from the roots of mycotrophic and non‐mycotrophic plant species

Schreiner

1993

New Phytologist

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SUMMARYThe roots of twelve plant species from both mycotrophic (hosts of VAM fungi) and non-mycotrophic (non-hosts) families were examined for their ability to produce antifungal compounds. A TLC bioassay was used with the fungus Cladospormm cucumerinum Ellis & Arth. Of the non-mycotrophic species, five species from the Brassicaceae [Brassica kaber (DC.) Wheeler, Brassica napus L., Brassica campestris L., Thlaspi arvense L., Rhaphanus raphanistrum L.] had detectable antifungal compounds in extracts from roots, while two species from the Chenopodiaceae {Spinacea oleracea L., Beta vulgaris L.) and one species from the Amaranthaceae (Amaranthus retroflexus L.) did not. One mycotrophic species (Daucus carota L.) also had a detectable antifungal compound in root extracts, while three other mycotrophic species {Lactuca sativa LK., Abutilon theophrasti Medic, Sorghum bicolor L.) did not. Chloroform extracts of B. kaber roots appeared to have the greatest quantity of extractable antifungal compounds as determined by the TLC bioassay. In a separate experiment, chloroform extracts from B. kaber roots greatly inhibited the germination of spores of Glomus etunicatum Becker and Gerd., while extracts from a number of other mycotrophic and non-mycotrophic plant roots were only marginally inhibitory. Three antifungal compounds in chloroform extracts from B. kaber roots were shown to be derived from glucosinolates. The predominant antifungal compound was identified as 4-hydroxybenzyl isothiocyanate, the isothiocyanate produced by the action of myrosinase on glucosinalbin. The data provide evidence of a possible role for glucosinolates in determining the non-mycotrophic status in B. kaber.

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“…Table 1 lists the compounds tested and their sources. Individual GLSs were characterised via NMR 'H and I3C and their purity checked by HPLC (Buchner, 1987).…”

Section: Preparation Of Glucosinolates and The Derived Itcsmentioning

confidence: 99%

In vitro activity of glucosinolate‐derived isothiocyanates against postharvest fruit pathogens*

Mari

Iori

Leoni

et al. 1993

Annals of Applied Biology

120

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The authors assayed the ability of some reaction products, essentially isothiocyanates, derived from the myrosinase-catalysed hydrolysis (neutral pH) of six natural glucosinolates to inhibit germination and mycelial growth of Botrytis cinerea, Rhizopus stolonifer, Monilinia laxa, Mucor piriformis and Penicillium expansum, the leading postharvest fungal pathogens of fruit and vegetable crops. All of the tested products showed antifungal activity, although they proved less effective against mycelial growth than in germination control. The isothiocyanates produced by enzymatic hydrolysis of glucoraphenin, sinalbin and sinigrin were particularly effective because they completely inhibited conidic germination of all five pathogens considered. The sinigrin-derived isothiocyanate exhibited a wide pathogen-control spectrum, either inhibiting conidia germination altogether or delaying by 3-6 days the onset of mycelial growth compared to the control.

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Comparison of Procedures for Optimum Extraction. Purification and Analysis of Desulfo Indolyl Glucosinolates

Cited by 8 publications

References 2 publications

In vitro activity of some glucosinolates and their reaction products toward a population of the nematode Heterodera schachtii

In vitro activity of some glucosinolates and their reaction products toward a population of the nematode Heterodera schachtii

Antifungal compounds from the roots of mycotrophic and non‐mycotrophic plant species

In vitro activity of glucosinolate‐derived isothiocyanates against postharvest fruit pathogens*

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