In vitro activity of glucosinolate‐derived isothiocyanates against postharvest fruit pathogens*

Mari, Marta; Iori, Renato; Leoni, Onofrio; Marchi, A.

doi:10.1111/j.1744-7348.1993.tb04082.x

Cited by 120 publications

(57 citation statements)

References 15 publications

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“…Major degradation products -catalyzed by the endogenous thioglucosidase enzyme (myrosinase) -include isothiocyanates, thiocyanates, and nitriles [Mikkelsen et al, 2002;Rask et al, 2000], which are all known to have antimicrobial effects [Brader et al, 2001;Manici et al, 1997;Mari et al, 1993;Tierens et al, 2001], while glucosinolates themselves do not have bactericidal effects as shown in our pure culture experiments with aliphatic 2-propenyl, aromatic benzyl glucosinolates and cruciferous plant extracts. Myrosinase is stored in idioblastic myrosin cells, while glucosinolates are located in the cell vacuoles in the surrounding tissue [Bones and Rossiter, 1995;Chen and Andreasson, 2001].…”

Section: Colonization Of Introduced E Radicincitans Cells In the Phymentioning

confidence: 86%

Interaction between Plants and Bacteria: Glucosinolates and Phyllospheric Colonization of Cruciferous Vegetables by Enterobacter radicincitans DSM 16656

Schreiner¹,

Krumbein²,

Ruppel³

2009

Microb Physiol

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For determining interactive plant-bacterial effects between glucosinolates and phyllospheric colonization by a plant growth-promoting strain, Enterobacter radicincitans DSM 16656, in cruciferous vegetables, the extent of bacterial colonization was assessed in 5 cruciferous vegetables (Brassica juncea, Brassica campestris, Brassica oleracea var. capitata, Brassica rapa var. alboglabra, Nasturtium officinale) using a species-specific TaqMan™ probe and quantitative real-time PCR. Colonization ability of inoculated E. radicincitans in the phyllosphere of these species varied from inability to colonize B. rapa up to a very good colonization rate of B. campestris. In addition to morphological factors and other plant compounds, the colonization rate was affected by different individual aromatic and aliphatic glucosinolates and their concentration, revealing that both plant pathogens and plant growth-promoting bacteria were affected by glucosinolates in their colonization behavior. In contrast, after E. radicincitans inoculation neither the total nor the individual glucosinolate concentrations in the phyllosphere of the 5 cruciferous species were affected, indicating that the nonpathogenic E. radicincitans might cause only poor cell damage by metabolizing plant cell components and does not induce a plant defense response and thus subsequently an increased glucosinolate concentration in the phyllosphere. Moreover, E. radicincitans induced no stimulation of indole glucosinolate biosynthesis by additional bacterial auxin supply.

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Section: Colonization Of Introduced E Radicincitans Cells In the Phymentioning

confidence: 86%

Interaction between Plants and Bacteria: Glucosinolates and Phyllospheric Colonization of Cruciferous Vegetables by Enterobacter radicincitans DSM 16656

Schreiner¹,

Krumbein²,

Ruppel³

2009

Microb Physiol

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“…Pathogen sensitivity and ecology 9.7.1 Inherent sensitivity to ITCs assessed using in vitro studies can vary by orders of magnitude for different pathogens , among isolates of the same pathogens (Smith & Kirkegaard, 2002), or for different life-cycle stages such as spores, conidia or mycelia (Mari et al, 1993). Thick-walled oospores or chlamydospores of various oomyces, or sclerotia of Rhizoctonia and Sclerotinia may be less sensitive to ITCs than germinating propagules of Aphanomyces euteiches or Fusarium oxysporum (Smolinska & Horbowicz, 1999).…”

Section: Ecological Considerations 97mentioning

confidence: 99%

Biofumigation for Plant Disease Control – from the Fundamentals to the Farming System

Kirkegaard

2009

Disease Control in Crops

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“…In 1993, inhibition of mycelial growth of several post-harvest fruit pathogens by BITC and another four natural ITCs was reported. 25 The authors reported that BITC concentrations of 3.7 mg ml −1 showed a low effectiveness (5%) against Penicillium expansum, Botrytis cinerea and Monilinia laxa. A few years later, Manici et al 26 reported that BITC showed a complete effectiveness near 0.55 mg ml −1 , against several fungi, including A alternata.…”

Section: In Vitro Antifungal Activity Of Bitc On a Alternatamentioning

confidence: 99%

Effect of benzyl isothiocyanate on tomato fruit infection development by Alternaria alternata

et al. 2005

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Benzyl isothiocyanate (BITC) is known to be a strong antifungal compound in vitro against different fungi. The effectiveness of benzyl isothiocyanate to control Alternaria alternata growth in vitro and in vivo was tested. BITC in vitro activity was evaluated in A alternata growing on potato dextrose agar and exposed to 0.025, 0.05, 0.1, 0.2 or 0.4 mg ml −1 . In vivo activity was evaluated by exposing A alternata-inoculated tomato fruits for either 18 or 36 h to 0.28 or 0.56 mg ml −1 BITC packed on low-density polyethylene film (LDPF) bags. Additionally, the effect of BITC on post-harvest physiology and tomato quality throughout storage at 20 • C was evaluated daily by monitoring respiration rate and ethylene production, whereas total soluble solids, pH, titratable acidity and fresh weight loss were measured every 3 days. Results showed that the minimal inhibitory concentration of BITC in vitro was 0.1 mg ml −1 . A combined use of 0.56 mg ml −1 BITC with LDPF for 18 h was the optimum treatment to control Alternaria rot in packed tomato fruit. No effect of BITC on respiration rate, ethylene production, total soluble solids, pH, weight loss and titratable acidity was observed. Results suggest that BITC can be used as a post-harvest treatment to control Alternaria rot in tomato fruit without detrimental effects on the tomato post-harvest quality.

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In vitro activity of glucosinolate‐derived isothiocyanates against postharvest fruit pathogens*

Cited by 120 publications

References 15 publications

Interaction between Plants and Bacteria: Glucosinolates and Phyllospheric Colonization of Cruciferous Vegetables by <i>Enterobacter radicincitans</i> DSM 16656

Interaction between Plants and Bacteria: Glucosinolates and Phyllospheric Colonization of Cruciferous Vegetables by <i>Enterobacter radicincitans</i> DSM 16656

Biofumigation for Plant Disease Control – from the Fundamentals to the Farming System

Effect of benzyl isothiocyanate on tomato fruit infection development by Alternaria alternata

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