Comparison of Procalcitonin Assays on KRYPTOR and LIAISON® XL Analyzers

Abstract

Our laboratory performs procalcitonin (PCT) assays on a Brahms KRYPTOR analyzer with the Brahms PCT sensitive Kryptor kit. In this study, we wanted to compare the assays obtained in this way with the ones performed on the LIAISON® XL. From January to May 2017, 171 samples were analyzed, of which 65 from female patients (age: 22–98 years) and 106 from male patients (age: 16–97 years). The PCT determination was performed using the LIAISON® XL and KRYPTOR analyzers, by chemiluminescence (Chemiluminescence immunoassay—CLIA) (LIAISON® BRAHMS PCT® II GEN) and immunofluorescence (Brahms PCT sensitive Kryptor) assay, respectively. For the LIAISON® BRAHMS PCT® II GEN, 52% of the results were placed between 0.0 and 0.5 ng/mL, 18% between 0.5 and 2.0 ng/mL, and 30% between 2.0 and 100 ng/mL; the mean was 4.09 ng/mL, the median 0.456 ng/mL, the maximum value 97.2 ng/mL, and the minimum value 0.02 ng/mL. For the Brahms PCT sensitive Kryptor, 55% of the results were positioned between 0.0 and 0.5 ng/mL, 21% between 0.5 and 2.0 ng/mL, and 24% between 2.0 and 100 ng/mL; the mean was 3.72 ng/mL, the median 0.39 ng/mL, the maximum value 103 ng/mL, and the minimum value 0.01 ng/mL. The mean of the results obtained with the two methods showed no significant differences (3.717 for Kryptor and 4.094 for LIAISON®). PCT assay with Brahms reagents, both on the Kryptor and LIAISON®XL platforms, offers excellent performance in terms of sensitivity and specificity.

Bir Akut Faz Protein Olan Prokalsitonin’in Biyobelirteç Olarak Veteriner Hekimlik Klinik Pratiğinde Kullanımı ve Önemi

“…İnsan kanındaki PCT konsantrasyonunu saptamak için, çoğunlukla kemilüminesans (21), immünofloresan (22), immünoluminometri (23) ve ayrıca yüzey plazmon rezonans biyosensörlerini (24) kullanan çeşitli yöntemler mevcuttur. Bu yöntemlerin tümü, aynı monoklonal fare anti-katakalsin antikorlarının ve monoklonal veya poliklonal koyun anti-kalsitonin antikorlarının (tahliller arasında farklıdır) bir kombinasyonuna dayanır.…”

Evaluation of the necessity and the feasibility of the standardization of procalcitonin measurements: Activities of IFCC WG-PCT with involvement of all stakeholders

“…However, not all assays commercially available take part in this harmonization effort, thus there is currently no commonly agreed reference material for all available PCT immunoassays at the international level. Therefore, the need for a new reference system based on higher order reference materials and/or a higher-order reference method to achieve global standardization/harmonization of PCT results worldwide has been pointed out in a number of studies [56][57][58][59]64,65].…”

Immunochromatography—Application Example and POCT Type Genetic Testing

“…Short detection time with 30 min is the biggest advantage. 39) Recently, quantitative assay test by automated immunofluorescence measuring instrument based on the chemiluminescence immunoassay method is available. 40) PCT attracts a lot of attention as a specific bacterial infection marker, 37) but sensitivity and specificity in Sepsis is not so significant compare to C-reactive protein (CRP).…”