Comparison of porcine and human coagulation by thrombelastometry

Kessler, Ulf; Grau, Tamar; Gronchi, Fabrizio; Berger, Steffen; Brandt, Sebastián; Bracht, Hendrik; Marcucci, Carlo; Zachariou, Zacharias; Jakob, Stephan M.

doi:10.1016/j.thromres.2011.03.013

Cited by 51 publications

(44 citation statements)

References 20 publications

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“…Although organ-specific differences (ie, lung oxygen reserve) may exist, these articles based their observations on different animal models (dog and pigs), and differences in the coagulatory response between the models may also account for this divergence, especially because pigs are known for their hypercoagulability, which makes it easier to induce disseminated intravascular coagulation in this model. 32,33 Furthermore, in this article, lungs were not transplanted at the end of EVLP. Although the evaluation of organ viability ultimately requires transplantation, we have provided physiological, histological, molecular, and ultrastructural evidence regarding the quality of these lungs with significant differences between the groups.…”

Section: Study Limitationsmentioning

confidence: 99%

Ex Vivo Lung Evaluation of Prearrest Heparinization in Donation After Cardiac Death

et al. 2013

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Section: Study Limitationsmentioning

confidence: 99%

Ex Vivo Lung Evaluation of Prearrest Heparinization in Donation After Cardiac Death

et al. 2013

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“…Overall, bleeding was the lowest valued parameter for all groups, which was expected as it is easier to achieve correct hemostasis in a porcine model than in a human patient, due to different cell biology, blood supply, and tissue textures 30.…”

Section: Discussionmentioning

confidence: 84%

Ureteral Obstruction Swine Model through Laparoscopy and Single Port for Training on Laparoscopic Pyeloplasty

Dı́az-Güemes¹,

Hernández-Hurtado²,

Usón-Casaús³

et al. 2013

Int. J. Med. Sci.

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This study aims firstly to assess the most adequate surgical approach for the creation of an ureteropelvic juntion obstruction (UPJO) animal model, and secondly to validate this model for laparoscopic pyeloplasty training among urologists.Thirty six Large White pigs (28.29±5.48 Kg) were used. The left ureteropelvic junction was occluded by means of an endoclip. According to the surgical approach for model creation, pigs were randomized into: laparoscopic conventional surgery (LAP) or single port surgery (LSP). Each group was further divided into transperitoneal (+T) or retroperitoneal (+R) approach. Time needed for access, surgical field preparation, wound closure, and total surgical times were registered. Social behavior, tenderness to the touch and wound inflammation were evaluated in the early postoperative period. After ten days, all animals underwent an Anderson-Hynes pyeloplasty carried out by 9 urologists, who subsequently assessed the model by means of a subjective validation questionnaire.Total operative time was significantly greater in LSP+R (p=0.001). Tenderness to the touch was significantly increased in both retroperitoneal approaches, (p=0.0001). Surgeons rated the UPJO porcine model for training on laparoscopic pyeloplasty with high or very high scores, all above 4 on a 1-5 point Likert scale.Our UPJO animal model is useful for laparoscopic pyeloplasty training. The model created by retroperitoneal single port approach presented the best score in the subjective evaluation, whereas, as a whole, transabdominal laparoscopic approach was preferred.

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“…This study used porcine blood, which has been previously described as a suitable model for researching ex vivo blood coagulation (Velik-Salchner et al, 2006). However, there are some differences between pig and human blood, most notably the structure of the platelet surface protein GPII b /III a which may be expected to influence total clot strength (Royo et al, 1998), although no such difference has previously been measured using TEG (Kessler et al, 2011). However, the findings of this study should be further validated with human blood to ensure that any adverse effects on haemostat performance measured in this study are not specific to pig blood.…”

Section: Discussionmentioning

confidence: 99%

Development of haemostatic decontaminants for the treatment of wounds contaminated with chemical warfare agents. 1: Evaluation of in vitro clotting efficacy in the presence of certain contaminants

Hall

Lydon

Dalton

et al. 2014

J of Applied Toxicology

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The treatment of penetrating, haemorrhaging injuries sustained within a hazardous environment may be complicated by contamination with toxic chemicals. There are currently no specific medical countermeasures for such injuries. Haemostats with an absorbent mechanism of action have the potential to simultaneously stop bleeding and decontaminate wounds. However, a primary requirement of a 'haemostatic decontaminant' is the retention of clotting function in the presence of chemical contaminants. Thus, the aim of this study was to investigate the haemostatic efficacy of seven commercially available haemostats in the presence of toxic chemicals (soman, VX, sulphur mustard, petrol, aviation fuel and motor oil). Clot viscosity was assessed ex vivo using thrombelastography following treatment of pig blood with: (i) toxic chemical; (ii) haemostat; or (iii) haemostat in combination with toxic chemical. Several contaminants (VX, petrol and GD) were found to be pro-haemostatic and none had an adverse effect on the rate with which the test products attained haemostasis. However, the total clot strength for blood treated with certain haemostats in the presence of sulphur mustard, soman and petrol was significantly decreased. Three test products failed to demonstrate haemostatic function in this ex vivo (thrombelastography) model; this was tentatively ascribed to the products achieving haemostasis through a tamponade mechanism of action, which can only be replicated using in vivo models. Overall, this study has identified a number of commercial products that may have potential as haemostatic decontaminants and warrant further investigation to establish their decontaminant efficacy.

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Comparison of porcine and human coagulation by thrombelastometry

Cited by 51 publications

References 20 publications

Ex Vivo Lung Evaluation of Prearrest Heparinization in Donation After Cardiac Death

Ex Vivo Lung Evaluation of Prearrest Heparinization in Donation After Cardiac Death

Ureteral Obstruction Swine Model through Laparoscopy and Single Port for Training on Laparoscopic Pyeloplasty

Development of haemostatic decontaminants for the treatment of wounds contaminated with chemical warfare agents. 1: Evaluation of in vitro clotting efficacy in the presence of certain contaminants

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