Comparison of Platelet-Rich Plasma Formulations for Cartilage Healing

Cavallo, Carola; Filardo, Giuseppe; Mariani, Erminia; Kon, Elizaveta; Marcacci, Maurilio; Ruiz, Maria Teresa Pereira; Facchini, Andrea; Grigolo, Brunella

doi:10.2106/jbjs.m.00726

Cited by 169 publications

(144 citation statements)

References 48 publications

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“…Bertrand-Duchesne et al [38], using a platelet concentrate collection system (PCCS), reported a platelet recovery rate of 63% (range, 44.1-75.0%); Leitner et al [39] compared different PRP systems with a collection efficiency ranging from about 13 and 78%. All these works support our data, nevertheless other articles report the number of recovered platelet as concentrations (number of platelets/volume) and usually do not mention any possible loss of cells and so cannot easily be compared with our data [40][41][42][43][44].…”

Section: Discussionsupporting

confidence: 83%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

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SummaryScientific background: Platelet concentrates are nowadays widely applied in different clinical fields to improve soft tissue and bone regeneration. "Concentrated Growth Factors" (CGF) is a new generation of platelet concentrate products, which exhibits an interesting clinical and biotechnological application potential. Aim of the study:The aim of this study is to assess the biological rationale for the use of CGF, by evaluating blood cell localization, the in vitro cumulative release of seven growth factors (PDGF-AB, VEGF, TNF-α, TGF-β1, BDNF, BMP-2 and IGF-1), its in vitro effects on cell proliferation and its mechanical behavior.Methods: CGFs were obtained from volunteer donors. Blood cell localization was evaluated after properly morphological staining and immunohistochemistry. The amount of growth factors release was measured at 5 hours, 1, 3, 6, 7 and 8 days, using ELISA assay. Cells were cultured with and without CGF and their proliferation was evaluated after 72 hours, performing the quantification of Ki-67, using flow cytometry (FACS). The mechanical response of CGF under compression was also attempted. Results:The results showed that platelets and leukocytes were found in a very thin space called "buffy coat", localized between the white and red part of CGF. Each growth factor evaluated, had a specific kinetic release with a great variability among subjects. The in vitro cell proliferation was stimulated. CGF showed an "apparent plasticity" and its mechanical response was influenced by fibrin network structure. Conclusion:These findings support the CGF's clinical use and will allow us to better understand and improve the clinical outcomes. B io lo g y and M e d ic in e

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Section: Discussionsupporting

confidence: 83%

Biological Characterization and In Vitro Effects of Human Concentrated Growth Factor Preparation: An Innovative Approach to Tissue Regeneration

Borsani¹,

Bonazza²,

Buffoli³

et al. 2015

Biol Med (Aligarh)

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“…Moreover, the different protocol used for PRP preparation can lead to products with different cellular components and biological characteristics. The difference in the sensibility of the method (modified agar diffusion method) used to evaluate the susceptibility to platelet concentrate should also be considered (Ehrenfest et al, 2013;Assirelli et al, 2014;Cavallo et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Antiviral Resistance of Splenocytes in Aged Mice

Zaczyńska

Artym

Kocięba

et al. 2017

Polish Journal of Microbiology

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A b s t r a c tAntimicrobial properties of platelet rich plasma (PRP) against various microorganisms have been recently pointed out. PRP could be an alternative to conventional antibiotics in preventing oral and periodontal infections. We examined whether PRP has in vitro antimicrobial properties against Aggregatibacter actinomycetemcomitans, Prophyromonas gingivalis, Staphylococcus aureus and Candida albicans. PRP and platelet-poor plasma (PPP) were obtained from whole blood of 10 healthy volunteers and 10 periodontitis patients. In vitro laboratory susceptibility was carried out using the modified agar diffusion method by measuring the diameters of inhibition zones on agar plates coated with selected microbial strains. Both calcium chloride (CaCl2) activated and non activated samples were tested. Both activated PRP and PPP, of both patients and controls, effectively inhibited the growth of A. actinomycetemcomitans, P. gingivalis and C. albicans. However, a statistically significant difference in favor of PRP was found indicating more susceptibility to PRP than PPP (p < 0.05). Non activated PRP and PPP exhibited negative zones of inhibition against the studied microorganisms. There was no activity against S. aureus. No statistically significant difference was found between the antimicrobial effects of PRP and/or PPP obtained from patients and controls (p > 0.05). We conclude that PRP is a potentially useful substance against oral and periodontal pathogens. Activated PRP was found to be more active than activated PPP and the activation of coagulation is a fundamental step. Additionally, the antimicrobial activity of PRP and/or PPP seems not to be affected by periodontitis.K e y w o r d s: antimicrobial activity, oral infection, platelet-rich plasma

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“…Although L-PRP has been shown to contain the highest levels of growth factors and cytokines 27 , it induces catabolic effects and a significantly greater acute inflammatory response and thus may actually prolong the healing process 27-30 . Thus, the inclusion of white cells defeats the purpose of PRP. On the other hand, P-PRP induces mainly anabolic changes, and while this is generally a beneficial outcome, it could also result in scar tissue formation due to these anabolic effects 27,30 . Still, no randomized or prospective clinical studies have been performed to compare outcomes between leukocyte-rich versus leukocyte-poor PRP.…”

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confidence: 99%