Comparison of Plasma, Saliva, and Hair Levetiracetam Concentrations

Karas-Ruszczyk, K; Kuczyńska, Julita; Sienkiewicz-Jarosz, Halina; Kurkowska‐Jastrzębska, Iwona; Bieńkowski, Przemysław; Restel, Magdalena; Samochowiec, Jerzy; Mierzejewski, Paweł

doi:10.1097/ftd.0000000000000396

Cited by 12 publications

(17 citation statements)

References 21 publications

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“…Many studies have confirmed that hair pigmentation has a relatively high binding capacity for basic drugs but not for acidic and neutral drugs. We showed that the hair colour or the dyeing of hair did not influence the levetiracetam concentration in the hair of patients 11 . Moreover, the main factors influencing drug incorporation in hair are the melanin affinity, lipophilicity and basicity (pK) of the drugs 38 …”

Section: Discussionmentioning

confidence: 91%

“…28,29 Our previous studies showed that there is a high correlation of saliva/plasma and, therefore, saliva can be applied as a matrix for levetiracetam and lamotrigine monitoring in patients with epilepsy. 11,12 However, no relationship was found for the dose/mg and concentration in hair relationship for these drugs. In the present study, we wanted to check the correlation between the concentration of the drug in the hair and the drug administration scheme in controlled conditions and to highlight the importance of hair as a matrix.…”

Section: Discussionmentioning

confidence: 98%

“…Compatibility between recommendations and real use by patients is different and depends on many factors, for example the drug dosage scheme, taking a dose of a drug as recommended (the patient may change the dose themself, eg based on their symptoms) or the duration of therapy 28,29 . Our previous studies showed that there is a high correlation of saliva/plasma and, therefore, saliva can be applied as a matrix for levetiracetam and lamotrigine monitoring in patients with epilepsy 11,12 . However, no relationship was found for the dose/mg and concentration in hair relationship for these drugs.…”

Section: Discussionmentioning

confidence: 99%

“…Choosing the most appropriate method is very important because we wanted to introduce the analysis of levetiracetam concentration in pulverized hair into routine diagnostics in patients with drug‐resistant epilepsy. In our other previous papers, we presented data on the clinical applications of an anti‐epileptic drug determination method in three biological matrices (plasma, saliva and hair) in patients with epilepsy 11,12 . Hair samples require additional preparation: decontamination, cutting into segments or pulverization 1,13,14 .…”

Section: Introductionmentioning

confidence: 99%

“…In our other previous papers, we presented data on the clinical applications of an anti-epileptic drug determination method in three biological matrices (plasma, saliva and hair) in patients with epilepsy. 11,12 Hair samples require additional preparation: decontamination, cutting into segments or pulverization. 1,13,14 Preparing a hair sample for the extraction of analytes from hair is one of the most important steps in hair analysis.…”

Section: Introductionmentioning

confidence: 99%

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Hair as a matrix in monitoring drug epilepsy therapy

Zakrzewska

Dermanowski

Mierzejewski

et al. 2020

Basic Clin Pharma Tox

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Hair is considered an efficient tool to investigate drug‐related histories; thus, the selection of the method of sample preparation is important to obtain a reliable result. The aim of this study was to compare two methods of hair preparation (cutting and pulverizing) to analyse levetiracetam concentration in hair. An additional aim was to evaluate the potential usefulness of the levetiracetam concentration measured as an index of a dosing schedule. Four groups of 12 rats were included in the experiment. Depending on the group, the rats received 10 mg/kg of levetiracetam intraperitoneally every 24, 48 and 72 hours for 30 days. The control group was not treated. At the end of the drug administration, the rats’ hair was shaved, cut or pulverized and analysed by the LC/MS‐MS method to determine the concentration of levetiracetam. A stronger correlation between the mean hair levetiracetam concentration in hair and the number of drug doses was found in pulverized hair than in cut hair. A smaller standard deviation between the results was obtained in the case of pulverized hair. The results indicate that pulverization gives a more reliable result of drug concentration in hair than cutting and that drug concentration in hair can reflect the scheme of levetiracetam administration.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hair as a matrix in monitoring drug epilepsy therapy

Zakrzewska

Dermanowski

Mierzejewski

et al. 2020

Basic Clin Pharma Tox

Self Cite

View full text Add to dashboard Cite

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Quantitation of levetiracetam concentrations in plasma and saliva samples by ultra‐performance liquid chromatography–tandem mass spectrometry: Application to therapeutic drug monitoring for pregnant women with epilepsy

Zhang,

Jin,

et al. 2023

Biomedical Chromatography

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Although levetiracetam (LEV) has favorable linear pharmacokinetic properties, therapeutic drug monitoring (TDM) is necessary for pregnant women with epilepsy. This study aims to build a simple, reliable, and sensitive ultra‐performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method for determining LEV concentrations in plasma and saliva samples, to support the routine TDM of LEV in Chinese pregnant women with epilepsy. The stable isotope‐labeled LEV‐d6 was used as the internal standard. The extracted samples were analyzed using a UPLC–MS/MS system with positive electrospray ionization. Mobile phase A was water containing 5 mM ammonium acetate and 0.1% formic acid, and phase B was 1:1 methanol–acetonitrile with 0.1% formic acid. The method was validated and utilized to determine LEV concentrations in non‐pregnant and pregnant patients with epilepsy. The developed method was validated in both plasma and saliva samples over a concentration range of 0.1–50 μg/mL. The intra‐ and inter‐batch accuracy for LEV ranged from −7.0% to 2.9%, with precisions between 2.7% and 9.3%. In pregnant patients, the mean dose‐standardized LEV trough plasma concentrations were significantly lower than those in non‐pregnant patients (4.73 ± 2.99 vs. 7.74 ± 3.59 ng/mL per mg/day; P < 0.0001). It is recommended that the TDM of LEV should be routinely performed during the different stages of pregnancy.

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