Comparison of pigment and proximate compositions of Tisochrysis lutea in phototrophic and mixotrophic cultures

Alkhamis, Yousef Ahmed; Qin, Jianguang

doi:10.1007/s10811-015-0599-0

Cited by 43 publications

(28 citation statements)

References 51 publications

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“…This strategy, termed OSMAC (One strain-many compounds), has successfully been applied for biodiscovery in bacteria (Bode et al, 2002) and could potentially also work in microalgae. The production of primary and secondary metabolites in microalgae can vary depending on e.g., growth phases (Vidoudez and Pohnert, 2012), clones (Gerecht et al, 2011), light (Depauw et al, 2012), temperature (Huseby et al, 2013), culturing media (Alkhamis and Qin, 2015), grazing pressure (Pohnert, 2002), extraction method (Jüttner, 2001), and probably many other factors (Chen et al, 2011). This metabolic plasticity positively influences drug discovery by triggering bioactivities and hopefully leading to the discovery of novel bioactive compounds for the treatment of human diseases.…”

Section: Discussionmentioning

confidence: 99%

Bioactivity Screening of Microalgae for Antioxidant, Anti-Inflammatory, Anticancer, Anti-Diabetes, and Antibacterial Activities

et al. 2016

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Marine microalgae are considered a potentially new and valuable source of biologically active molecules for applications in the food industry as well as in the pharmaceutical, nutraceutical, and cosmetic sectors. They can be easily cultured, have short generation times and enable an environmentally-friendly approach to drug discovery by overcoming problems associated with the over-utilization of marine resources and the use of destructive collection practices. In this study, 21 diatoms, 7 dinoflagellates, and 4 flagellate species were grown in three different culturing conditions and the corresponding extracts were tested for possible antioxidant, anti-inflammatory, anticancer, anti-diabetes, antibacterial, and anti-biofilm activities. In addition, for three diatoms we also tested two different clones to disclose diversity in clone bioactivity. Six diatom species displayed specific anti-inflammatory, anticancer (blocking human melanoma cell proliferation), and anti-biofilm (against the bacteria Staphylococcus epidermidis) activities whereas, none of the other microalgae were bioactive against the conditions tested for. Furthermore, none of the 6 diatom species tested were toxic on normal human cells. Culturing conditions (i.e., nutrient starvation conditions) greatly influenced bioactivity of the majority of the clones/species tested. This study denotes the potential of diatoms as sources of promising bioactives for the treatment of human pathologies.

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Section: Discussionmentioning

confidence: 99%

Bioactivity Screening of Microalgae for Antioxidant, Anti-Inflammatory, Anticancer, Anti-Diabetes, and Antibacterial Activities

et al. 2016

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“…Instead, the production of primary and secondary metabolites is clearly variable, regulated or modulated by, e.g. light (Depauw et al 2012 ), temperature (Thomson et al 1992 ; Huseby et al 2013 ), growth phase stage (Barofsky et al 2009 , 2010 ; Vidoudez and Pohnert 2012 ), strain or species (Degerlund et al 2012 ), grazing (Pohnert 2002 ), culture media (Alkhamis and Qin 2015 ), extraction method (Jüttner 2001 ), freezing and thawing of samples (Eilertsen et al 2014 ), clonal variability (Gerecht et al 2011 ) various stress and probably many other factors (Chen et al 2011 ). This variability complicates interpretation of results and makes it very difficult to make general statements about which factor controls production of, e.g.…”

Section: Discussionmentioning

confidence: 99%

Light and temperature effects on bioactivity in diatoms

et al. 2015

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Isolates of five pelagic North Atlantic marine diatoms (Bacillariophyceae): Attheya longicornis, Chaetoceros socialis, Chaetoceros furcellatus, Skeletonema marinoi and Porosira glacialis were cultivated in large photobioreactors at two light and two temperature regimes to test if this affected bioactivity. We screened for bioactivity in assays representing five different therapeutic areas: diabetes II (PTP1b), cancer (melanoma cells, A2058), anti-oxidants (FRAP), immunomodulation (TNFa) and anti-infection (MRSA, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa). All the diatom strains showed activity in two or more assays. We detected differences in bioactivity both between species and within species cultivated with different light and temperature regimes. Our results demonstrate the potential for a more exhaustive exploitation of diatom metabolites that can be obtained by manipulation of the cultivation conditions.

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“…Total nitrogen (N) was determined by the Kjeldahl micro-method using 100 mg of lyophilized biomass (Bremner 1965 ). Total protein contents were calculated using the total N data and conversion factors of 6.25, according to Alkhamis and Qin ( 2015 ), and 4.92, as recommend for green, brown, and red marine algae by Lourenço et al ( 2002 ). All assays were conducted in triplicate.…”

Section: Methodsmentioning

confidence: 99%

“…Biomolecules can be obtained as byproducts from microalgae during biofuel production, and their subsequent utilization in animal and aquaculture feed might sustain an industrial-scale cultivation system (Yaakob et al 2014 ). A mixotrophic system is defined as one in which organic carbon sources, such as molasses, glucose, glycerol, sucrose, lactose, starch, and CO 2 , are simultaneously assimilated by respiratory and photosynthetic metabolic pathways (Alkhamis and Qin 2015 ; Rai et al 2013 ; Yeesang and Cheirsilp 2014 ). A mixotrophic system combines the advantages of phototrophic and heterotrophic cultures (Li et al 2014 ), and obtains energy from organic and inorganic carbon sources, as well as light.…”

Section: Introductionmentioning

confidence: 99%

“…A mixotrophic system combines the advantages of phototrophic and heterotrophic cultures (Li et al 2014 ), and obtains energy from organic and inorganic carbon sources, as well as light. In mixotrophic cultivation, microalgal cultures produce more biomass (Babuskin et al 2014 ), including higher amounts of pigments and fatty acids, than phototrophic systems (Alkhamis and Qin 2015 ).…”

Section: Introductionmentioning

confidence: 99%

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Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions

et al. 2016

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Mixotrophic cultivation is a potential approach to produce microalgal biomass that can be used as raw materials for renewable biofuels and animal feed, although using a suitable, cost-effective organic carbon source is crucial. Here, we used a Box–Behnken design with three factors, the glucose and sodium acetate concentrations, and the percentage of Bold’s basal medium (BBM), to evaluate the effects of different carbon sources on biomass productivity and the protein and lipid contents of Neochloris oleoabundans (UTEX#1185). When grow at optimal levels of these factors, 100 % BBM plus 7.5 g L−1 each of glucose and sodium acetate, N. oleoabundans yielded 1.75 g L−1 of dry biomass, with 4.88 ± 0.09 % N, 24.01 ± 0.29–30.5 ± 0.38 % protein, and 34.4 % ± 0.81 lipids. A nuclear magnetic resonance spectrum (1H-NMR) of a lipid extract showed that the free fatty acid content was 11.25 %. Thus, combining glucose and sodium acetate during the mixotrophic cultivation of N. oleoabundans can yield greater amounts of biomass, proteins, and lipids for biofuel production.

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Comparison of pigment and proximate compositions of Tisochrysis lutea in phototrophic and mixotrophic cultures

Cited by 43 publications

References 51 publications

Bioactivity Screening of Microalgae for Antioxidant, Anti-Inflammatory, Anticancer, Anti-Diabetes, and Antibacterial Activities

Bioactivity Screening of Microalgae for Antioxidant, Anti-Inflammatory, Anticancer, Anti-Diabetes, and Antibacterial Activities

Light and temperature effects on bioactivity in diatoms

Combining glucose and sodium acetate improves the growth of Neochloris oleoabundans under mixotrophic conditions

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