Comparison of phytochemical profiles, cellular antioxidant and anti‐proliferative activities in five varieties of wampee (Clausena lansium) fruits

Abstract

Summary Wampee (Clausena lansium) fruit is a seasonal food containing high levels of bioactive phytochemicals which may be useful for health benefits. This study compared the phytochemical composition, total and cellular antioxidant activities (CAA) as well as anti‐proliferative activities of five different varieties of wampee fruits. The results showed that six phytochemical compounds (vanillic acid, ferulic acid, rutin, syringin, catechin and hesperetin) were found in wampee fruits. Total antioxidant activities varied dramatically in these five varieties when measured with oxygen radical absorbance capacity assay and peroxyl radical scavenging capacity assay. The results also showed that wampee fruit extracts varied enormously in their CAA and exhibited significant anti‐proliferative activities against HepG2 human liver cancer cells. These results suggest the potential of wampee fruits as functional foods and industrial application of wampee fruit in the future.

Comparison of Phytochemical Profile, Mineral Content, andIn VitroAntioxidant Activities ofCorchorus capsularisandCorchorus olitoriusLeaf Extracts from Different Populations

“…TPC was a positive correlation obtained with FRAP at the 1% level. Similar trends were reported by [61], who noticed that phenolic content had significant positive correlations with FRAP (r � 0.992, p < 0.01) in wampee fruits.…”

“…Pearson's correlation coefficient was performed in order to illuminate the relationship of all of the measured parameters. Different literature reported the Pearson correlations among the secondary metabolites and antioxidant activity in several plant species [60,61]. Analytical results indicated that the best correlation was between TFC and FRAP (0.731 * * ) as well as between TPC and FRAP (0.590 * * ), followed by TFC and ABTS (0.573 * * ) ( Table 3).…”

Extraction of flavanones from immature Citrus unshiu pomace: process optimization and antioxidant evaluation

“…Nitrogen radical scavenging activities (DPPH and ABTS) were determined. DPPH radical scavenging activity was evaluated as described by Ye et al 26 . Each extract (0.1 mL) was mixed with 2.0 mL of a DPPH solution (0.2 mM) and left for 30 min.…”

“…ROS scavenging activities (ORAC, hydroxyl radical, superoxide anion radical, hydrogen peroxide) were determined. ORAC was measured as the methods described by Ye et al 26 and Kim and Lim 25 . Each extract (0.025 mL) was mixed with 0.15 mL of fluorescein sodium salt solution (78 nM), left for 15 min at 37 °C, and added with 0.025 mL of AAPH (250 mM).…”

“…The absorbance was determined at 700 nm. Ferric reducing antioxidant power (FRAP) was evaluated as the method described by Ye et al 26 . The FRAP solution was prepared with 300 mM acetate buffer, 20 mM FeCl 3 , and 10 mM TPTZ (1:1:10 (v/v)).…”