Comparison of photodynamic efficacy of tetraarylporphyrin pegylated or encapsulated in liposomes: In vitro studies

Nawalany, Kinga; Rusin, Aleksandra; Kępczyński, Mariusz; Mikhailov, Alexei; Kramer‐Marek, Gabriela; Śnietura, Mirosław; Połtowicz, Jan; Krawczyk, Z; Nowakowska, Maria

doi:10.1016/j.jphotobiol.2009.07.005

Cited by 38 publications

(30 citation statements)

References 32 publications

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“…Moreover, the pegylated porphyrin dissolved in culture medium was less readily taken-up by cells than the porphyrin encapsulated in liposomes, probably due to formation of large polymeric clusters. Additionally, the liposomal formulation showed higher photocytotoxicity than p-THPP-PEG 2000 towards both cell lines, but the overall phototoxicity efficiency was dependent on the type of the cancer cell line (Nawalany et al 2009).…”

Section: The Role Of Photosensitizer Nanocarriers For Pdt 1107mentioning

confidence: 92%

“…I n a n o t h e r s t u d y, c a r r i e d o u t b y Nawalany et al (Nawalany et al 2009), other photosensitizing systems based on the synthetic meso-tetraarylporphyrins: (1) 5,10,15,20-tetrakis(4-hydroxyphenyl)porphyrin (p-THPP) encapsulated in sterically stabilized liposomes (N-[methoxy(polyethyleneglycol) 2000 ] c a r b o n y l -1 , 2 -d i p a l m i t o y l -s n -g l y c e r o -3 -phosphoethanolamine sodium salt) and (2) p-THPP covalently attached to polyethylene glycol (PEG 2000 ) were studied in vitro (Figure 4a). The dark and photocytotoxicity of both systems were evaluated on two cell lines: a human colorectal carcinoma cell line (HCT 116) and a prostate cancer cell line (DU 145), and compared with free p-THPP.…”

Section: The Role Of Photosensitizer Nanocarriers For Pdt 1107mentioning

confidence: 99%

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An insight on the role of photosensitizer nanocarriers for Photodynamic Therapy

Mesquita

Dias

Gamelas

et al. 2018

An. Acad. Bras. Ciênc.

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Photodynamic therapy (PDT) is a modality of cancer treatment in which tumor cells are destroyed by reactive oxygen species (ROS) produced by photosensitizers following its activation with visible or near infrared light. The PDT success is dependent on different factors namely on the efficiency of the photosensitizer deliver and targeting ability. In this review a special attention will be given to the role of some drug delivery systems to improve the efficiency of tetrapyrrolic photosensitizers to this type of treatment.

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Section: The Role Of Photosensitizer Nanocarriers For Pdt 1107mentioning

confidence: 92%

Section: The Role Of Photosensitizer Nanocarriers For Pdt 1107mentioning

confidence: 99%

An insight on the role of photosensitizer nanocarriers for Photodynamic Therapy

Mesquita

Dias

Gamelas

et al. 2018

An. Acad. Bras. Ciênc.

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“…39 The intracellular localizations of free DD-PpIX and FA-PEG-P(Asp-Hyd) 50 -DD-PpIX 1.0 nanoparticles in HeLa cells were investigated using flow cytometry and confocal laser scanning microscopy. Figure 6 shows the flow cytometry assay results.…”

Section: Cellular Localization Of Dd-ppix-conjugated Nanoparticlesmentioning

confidence: 99%

Self-assembled photosensitizer-conjugated nanoparticles for targeted photodynamic therapy

et al. 2012

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An effective tumor-targeted drug delivery system for photodynamic therapy was developed by designing ligand-mediated nanoparticles with stable formulations of a hydrophobic photosensitizer. Novel folic acid (FA)-conjugated amphiphilic block copolymers of polyethylene glycol (PEG) and poly-β-benzyl-L-aspartate (PBLA) with the potential to act as pH-responsive drug release reservoirs were synthesized. The photosensitizer, 2,4-diacetyl deuteroporphyrin IX dimethyl ether (DD-PpIX), was conjugated to the copolymers through pH-sensitive hydrazone linkage. The syntheses and compositions of all copolymers were confirmed by ¹H NMR measurement. Photosensitizer-conjugated amphiphilic copolymeric nanoparticles (FA-PEG-P(Asp-Hyd)-DD-PpIX) were prepared by micelle formation in aqueous solution. The particle sizes of the FA-PEG-PBLA and FA-PEG-P(Asp-Hyd)-DD-PpIX nanoparticles were determined by light-scattering measurements. The range was 105-298 nm, depending on copolymer molecular weight and composition. Field emission scanning electron microscopy showed that the FA-PEG-P(Asp-Hyd)-DD-PpIX copolymeric nanoparticles were submicron in size and spherical in shape. The results of in vitro release tests showed that the release profiles of DD-PpIX from the nanoparticles were strongly pH-dependent and influenced by the amount of photosensitizer that was conjugated. In vitro tests using HeLa cells indicated that the FA-PEG-P(Asp-Hyd)-DD-PpIX nanoparticles had low dark-toxicity and showed more than 97% of cellular uptake. Based on our results, the FA-PEG-P(Asp-Hyd)-DD-PpIX nanoparticle system could be a promising approach for developing novel photosensitizer delivery carriers for photodynamic therapy.

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“…We were able to find only one paper that reported some comparison studies between two different systems. Comparison of pegylated porphyrin with the free porphyrin incorporated into nanostructure system was performed by Nawalany et al [80]. Two photosensitizing systems were studied in vitro in this paper.…”

Section: Liposomal Formulationsmentioning

confidence: 99%

Nanostructural Hybrid Sensitizers for Photodynamic Therapy

Kępczyński

Dzieciuch²,

Nowakowska³

2012

CPD

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Photodynamic therapy (PDT) is a clinically approved method for treatment of cancer, microbial infections, and some other diseases. PDT has proved effective in the treatment of malignancies of various organs, including lung, bladder, gastrointestinal tract, and skin, and in the therapy of bacterial infection of skin wounds and carious lesions. It employs a combination of light and a drug (photosensitizer, PS) to induce phototoxicity against cancerous cells or bacteria. The efficiency of currently used PSs is limited due to their hydrophobic nature, which causes aggregation of the PS in aqueous media and low tumor selectivity (a low value of the tumor-to-normal tissue ratio). The purpose of this review is to present some aspects of the current state of knowledge on nanostructural carriers for the PS delivery. In this paper we reviewed studies on the development of nanostructural materials for PDT, especially those based on the polymeric and liposomal formulation of PS. We focused mainly on the nanostructural PSs obtained by the covalent attachment of hydrophilic polymer chain to the low-molecular-weight PS, the incorporation of PS into polymeric nanostructures such as micelles, and the solubilization of PS in liposome carriers.

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Comparison of photodynamic efficacy of tetraarylporphyrin pegylated or encapsulated in liposomes: In vitro studies

Cited by 38 publications

References 32 publications

An insight on the role of photosensitizer nanocarriers for Photodynamic Therapy

An insight on the role of photosensitizer nanocarriers for Photodynamic Therapy

Self-assembled photosensitizer-conjugated nanoparticles for targeted photodynamic therapy

Nanostructural Hybrid Sensitizers for Photodynamic Therapy

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