Comparison of Phenytoin and Carbamazepine Serum Concentrations Measured by High-Performance Liquid Chromatography, the Standard TDx Assay, the Enzyme Multiplied Immunoassay Technique, and a New Patient-Side Immunoassay Cartridge System

Rambeck, B.; Tw, May; Mu, Jürgens; Blankenhorn,; Jürges, Uta; Korn-Merker, E; Sälke-Kellermann, A.

doi:10.1097/00007691-199412000-00013

Cited by 18 publications

(5 citation statements)

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“…The above ndings almost match results of Rambeck et al, who found that in the case of PHT there was a highly linear correlation (r = 0.985, y = 1.113x -0.589) between HPLC and the Biotrack system, while in the case of CBZ, the correlation between HPLC and Biotrack system was somewhat lower (r = 0.931, y = 1.29x -0.136) (14). Similar results of correlation have found others authors, too (15,16).…”

Section: Discussionsupporting

confidence: 89%

Comparison between the immunoassay and high performance liquid chromatography for therapeutic monitoring of carbamazepine and phenytoine

2010

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Objective: To investigate the correlation of the immunoassay and chromatography method for quantitative measurement of two antiepileptic drugs (AED), carbamazepine (CBZ) and phenytoin (PHT) and determination of relation between the CBZ and it's metabolite carbamazepine 10,11-epoxide (CBZ-E). Additionally we investigated whether there is a di erence in the determination of serum concentration of CBZ and PHT when measured in two di erent labs by high performance liquid chromatography (HPLC). Materials and methods:This study was carried out on 102 blood samples (72 CBZ and 30 PHT) collected from epileptic outpatients. Plasma concentrations of CBZ and PHT were determined by validated HPLC (Shimadzu and Agilent) and the CEDIA-immunoassay method. Results: The correlations of serum concentrations of CBZ between CEDIA and HPLC1 and between CEDIA and HPLC2 were good (R = 0.97 for both techniques). Even better correlation was found between concentrations of CBZ measured by the two HPLC systems (R = 0.99). Similar, for PHT, we found good correlation between CEDIA and the two systems of HPLC (HPLC1 and HPLC2, R = 0.98) and between the two systems of HPLC of R =0.98. The moderate correlation coe cient was found between serum concentrations of CBZ and its metabolite CBZ-E, measured in two labs by di erent HPLC (R = 0.49 and 0.43, respectively; P < 0.001). Conclusion:We observed good correlation for estimation of CBZ and PHT concentration obtained by means the immunoassay and two di erent HPLC. The possibility of measurement of CBZ-E could be advantage of chromatography in comparison with immunoassay.

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Section: Discussionsupporting

confidence: 89%

Comparison between the immunoassay and high performance liquid chromatography for therapeutic monitoring of carbamazepine and phenytoine

2010

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“…Currently, a number of analytical methods for the monitoring of antiepileptic drugs in biological samples such as HPLC with UV detection , evaporative light‐scattering detection , fluorescence polarization immunoassay , and enzyme‐multiplied immunoassay technique have been reported but many suffer from disadvantages such as relatively long analytical run time, large sample volumes, and complex sample preparation. Various LC–MS‐based methods for one or several antiepileptic drugs have been developed and used in clinical laboratories but limited by the monitoring numbers of antiepileptic drugs or long run time which is not suitable for high‐throughput analysis.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of ten antiepileptic drugs in human plasma by liquid chromatography and tandem mass spectrometry with positive/negative ion‐switching electrospray ionization and its application in therapeutic drug monitoring

Yin

Wang

Shi

et al. 2016

J of Separation Science

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A simple, rapid, and high-throughput liquid chromatography with tandem mass spectrometry method for the simultaneous quantitation of ten antiepileptic drugs in human plasma has been developed and validated. The method required only 10 μL of plasma. After simple protein precipitation using acetonitrile, the analytes and internal standard diphenhydramine were separated on a Zorbax SB-C18 column (50 × 4.6 mm, 2.7 μm) using acetonitrile/water as the mobile phase at a flow rate of 0.9 mL/min. The total run time was 6 min for each sample. The validation results of specificity, matrix effects, recovery, linearity, precision, and accuracy were satisfactory. The lower limit of quantification was 0.04 μg/mL for carbamazepine, 0.02 μg/mL for lamotrigine, 0.01 μg/mL for oxcarbazepine, 0.4 μg/mL for 10-hydroxycarbazepine, 0.1 μg/mL for carbamazepine-10,11-epoxide, 0.15 μg/mL for levetiracetam, 0.06 μg/mL for phenytoin, 0.3 μg/mL for valproic acid, 0.03 μg/mL for topiramate, and 0.15 μg/mL for phenobarbital. The intraday precision and interday precision were less than 7.6%, with the accuracy ranging between -8.1 and 7.9%. The method was successfully applied to therapeutic drug monitoring of 1237 patients with epilepsy after administration of standard antiepileptic drugs. The method has been proved to meet the high-throughput requirements in therapeutic drug monitoring.

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“…Meanwhile, determination of CBZ in plasma or serum has been developed by Jens Martens (1993) and B. Rambeck et al (1994). 21,22 This method has been a traditional and simple one for in vitro diagnostic use in the quantitative analysis of CBZ in a hospital. However, the process of EMIT suffered from many inuencing factors (temperature, reaction time and so on).…”

Section: Comparison Of C T From Clinical Samples By Hfcf-uf Ppt and mentioning

confidence: 99%

The significance of a new parameter – plasma protein binding – in therapeutic drug monitoring and its application to carbamazepine in epileptic patients

Gao

Wang

et al. 2017

RSC Adv.

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Comparison of Phenytoin and Carbamazepine Serum Concentrations Measured by High-Performance Liquid Chromatography, the Standard TDx Assay, the Enzyme Multiplied Immunoassay Technique, and a New Patient-Side Immunoassay Cartridge System

Cited by 18 publications

References 0 publications

Comparison between the immunoassay and high performance liquid chromatography for therapeutic monitoring of carbamazepine and phenytoine

Comparison between the immunoassay and high performance liquid chromatography for therapeutic monitoring of carbamazepine and phenytoine

Simultaneous determination of ten antiepileptic drugs in human plasma by liquid chromatography and tandem mass spectrometry with positive/negative ion‐switching electrospray ionization and its application in therapeutic drug monitoring

The significance of a new parameter – plasma protein binding – in therapeutic drug monitoring and its application to carbamazepine in epileptic patients

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