Comparison between the immunoassay and high performance liquid chromatography for therapeutic monitoring of carbamazepine and phenytoine

Krasniqi, Shaip; Zeitlinger, Markus; Bauer, Steffen

doi:10.11613/bm.2010.044

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…12,13 Although several attempts were made to replace the standard high-performance liquid chromatography (HPLC) methods with faster and simpler alternatives, there is a general consensus that TDM needs highly specific, robust, and validated methods for obtaining reliable results, the latter being essential in case of pharmacokinetic studies. [13][14][15] Others proposed more convenient collection methods of biological samples in order to increase TDM feasibility. Dried blood-spot (DBS) was an innovative surrogate for classic blood sample collection, as highly specific tandem mass spectrometry detection enabled the determination of the analyte of interest from very small sample volumes.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Carbamazepine and Carbamazepine-10,11-epoxide in Different Biological Matrices by LC-MS/MS

Andonie

Gáll

Bosa

et al. 2017

Journal of Interdisciplinary Medicine

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An uncomplicated, sensitive liquid chromatography linked to mass spectrometry (LC/MS) for evaluation of carbamazepine and carbamazepine-10,11-epoxide (its metabolite) in human plasma, human saliva, rat plasma, and rabbit plasma was developed. Analyses were conducted on a Zorbax SB-C18, 100 mm × 3 mm ID, 3.5 μm column, at a column temperature of 40 ºC. The mobile phase was comprised of 0.1% formic acid in water and methanol in a 35 : 65 (v/v) ratio, with a flow rate of 0.4 mL/min. Lacosamide was utilized as internal standard. Under these chromatographic conditions, the retention times of lacosamide, carbamazepine-10,11-epoxide, and carbamazepine were 1.4 min, 1.6 min, and 2.2 min, respectively. The quantification of the analytes was performed using multiple reaction monitoring, with the use of a triple quadrupole mass spectrometer with electrospray positive ionization. The monitored ions were m/z 194 derived from m/z 237 for carbamazepine, m/z 180 derived from m/z 253 for carbamazepine-10,11-epoxide, and m/z 108 derived from m/z 251 for lacosamide. The samples were prepared by protein precipitation from 0.2 mL of plasma/saliva using 0.6 mL of internal standard solution in methanol. Calibration curves were constructed over the ranges 1.1-17.6 μg/mL and 0.23-5.47 μg/mL for carbamazepine and carbamazepine-epoxide, respectively. The coefficients of determination obtained by using a weighted (1/x) linear regression were greater than 0.994. The reported LC-MS/MS method was applied to preclinical pharmacokinetic studies and therapeutic drug monitoring.

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